PHOTOSYNTHESI S Ch 6 ATP Remember ATP is
PHOTOSYNTHESI S Ch 6
ATP • Remember… • ATP is cellular energy • Energy from the chemical bonds of ATP is released when the bond between the 2 nd and 3 rd phosphate groups is broken. • It then becomes adenosine DIphosphate – ADP • ATP is then recharged! It uses energy to gain an extra P and become ATP again
Metabolism • Anabolism: metabolic reactions that build molecules [endergonic reaction] • Catabolism: metabolic reactions that break down molecules [exergonic reaction] • Life is sustained by inputs of energy, however not all forms of energy can sustain life. • The sun is abundant, but cannot directly power protein synthesis or energy-requiring reactions, it must first be converted to chemical bond energy
Photosynthesis • Energy flow through ecosystems begins when photosynthesizers [plants] capture sunlight and convert it to chemical energy • This chemical energy can power the reactions of life, and can be stored for use at a later time
Photosynthesis • Autotrophs are producers • Make food using energy from environment and carbon from inorganic molecules • Heterotrophs are consumers • Obtain carbon from organic compounds assembled by other organisms
Photosynthesis • Photosynthesis: Metabolic pathway that uses light energy to turn carbon dioxide (CO 2) and water (H 2 O) into carbohydrates (sugar) • Also creates OXYGEN! • The process is cyclical, the products from one reaction are the reactants for the next reaction
Photosynthesis • Photosynthesis has 2 parts • Light-dependent: converts light energy into chemical energy; produces oxygen and ATP to be used in light-independent reaction • Light-independent: does not require light energy, uses CO 2 and H 2 O to build sugars; powered by ATP, also called the CALVIN CYCLE
Chloroplasts • Photosynthesis occurs in the chloroplast • The chloroplast is a plastid that carries out photosynthesis • Plastids are double membrane organelles that contain pigment • Chloroplasts resemble the photosynthetic bacteria they evolved from, so photosynthesis in eukaryotic cells is similar to bacterial photosynthesis
Chloroplasts • Thylakoid membrane: Highly folded into stacks of interconnected thylakoids [Light-dependent reactions] • Folds in this membrane form disks called thylakoids • The membrane encloses a single, continuous internal space • Stroma: Cytoplasm-like fluid inside the chloroplast [Light-independent reactions] • thylakoid membrane, ribosomes, and chloroplast’s DNA are suspended in the stroma
Photosynthesis • Pigment: organic molecule that selectively absorbs certain wavelengths of light • If a wavelength is not absorbed, it is reflected, and that gives the pigment its color • Chlorophyll a is the most common pigment in plants • It absorbs violet, red, and orange light, but reflects green!
Photosynthesis • Most photosynthetic cells have accessory pigments that capture other wavelengths of light • Other functions (attract pollinators; antioxidants) • Disassembly of chlorophylls during autumn reveals accessory pigments
Phase 1: Light Reaction 1. Light hits a leaf, causing an electron in chlorophyll to enter an excited state (higher energy level) within the chloroplast’s thylakoid membrane. 2. The excited electrons leaves the chlorophyll and an enzyme splits a water molecule to replace the electron lost by the chlorophyll. • Oxygen gas is formed and exits through the membrane and into the atmosphere. H+ ions accumulate inside thylakoid space. Ferredoxin T GH LI O 2 H 2 O H+
3. The released electron goes through electron transport chain. The electron is bounced from between proteins in the thylakoid membrane. Note: Energy lost along electron transport chain 4. The electron is eventually passed to another chlorophyll molecule and then on to the final electron acceptor (ferredoxin NADP+ reductase) where the electron transport chain ends. 5. Ferredoxin NADP+ reductase then gives the electron to an electron carrier NADP which turns it into NADPH carriers the electron to the next phase. NADP O 2 NADPH Ferredoxin H+ H+ H+
6. As more copies of steps 1 through 5 occur, more and more H+ ions from step 2 begin to build up in the thylakoid space. H+ ions begin to pass from an area of higher concertation (thylakoid space) to an area of lower concentration (stroma) through an enzyme called ATP synthase. This process called, chemiosmosis, creates ATP. H+ H+ ADP ATP Ferredoxin H+ H+
Phase 1: Light Reaction Summary • Lost energy used to recharge ATP from ADP • NADPH produced from e- transport chain • Stores energy until transfer to stroma • Plays important role in light-independent reaction (LIR) • Total byproducts: ATP (Used in LIR), NADPH (Used in LIR), O 2 (Exits plant)
Phase 2: Dark Reaction (Light. Independent) • In the second phase, also called the Calvin-Benson Cycle, carbon dioxide, ATP and NADPH are used to make sugar (glucose) in the stroma. • The “synthesis” part • Plants must make glucose to store energy long term. ATP is only short term energy!
Phase 2: Dark Reaction 1. In the stroma, an enzyme called RUBISCO takes a few molecules of carbon dioxide and connects it to a molecule called Ru. BP and turns it into a 3 carbon molecule called PGA 2. Then more enzymes use ATP and NADPH from the light reactions to rearrange the molecules in PGA to store energy and create a few molecules called G 3 P. 3. NOTE: When ATP and NADPH are used as energy it they become ADP and NADP respectively. The NADP and ADP are sent back to the light reactions to get recharged. 4. Some G 3 P’s are sent into the cytoplasm to make glucose. Some G 3 P molecules are used to make Ru. BP that are needed to restart the dark reactions.
H 2 O Light Reactions O 2 Chloroplast ATP CO 2 Calvin Cycle Sugar (Glucose)
Alternative Pathways • Plants in some climates have alternative ways of doing photosynthesis to help them survive better.
Alternative Pathways Alternatives
Rate of Photosynthesis • Light intensity, carbon dioxide levels, and temperature effect the rate of photosynthesis • As light increases, rate of photosynthesis increases • As CO 2 increases, rate of photosynthesis increases • Temperature Low = Rate of photosynthesis low • Temperature Increases = Rate of photosynthesis increases • If temperature too hot, rate drops
Chemosynthesis • The synthesis of organic compounds by bacteria or other living organisms using energy from reactions involving inorganic chemicals, typically in the absence of sunlight. • They use chemicals instead of water as an electron donor before the electron transport chain. • They generally don’t produce oxygen. • Example: Hydrogen sulfide eating bacteria in deep ocean vents.
Light-Independent Reactions • The Calvin–Benson cycle • Builds sugars in the stroma of chloroplasts • Not powered by light energy • Driving force is ATP and NADPH formed by the lightdependent reactions • Uses carbon atoms from CO 2 to make sugars
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