Pharmacogenetics Dr Mohammed AlSbou Professor of Clinical Pharmacology
![Pharmacogenetics Dr. Mohammed Al-Sbou Professor of Clinical Pharmacology Faculty of Medicine-Mutah Uni 1 Pharmacogenetics Dr. Mohammed Al-Sbou Professor of Clinical Pharmacology Faculty of Medicine-Mutah Uni 1](https://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-1.jpg)
Pharmacogenetics Dr. Mohammed Al-Sbou Professor of Clinical Pharmacology Faculty of Medicine-Mutah Uni 1
![n n Human beings are 99, 9% genetically identical The human genome project has n n Human beings are 99, 9% genetically identical The human genome project has](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-2.jpg)
n n Human beings are 99, 9% genetically identical The human genome project has led to an explosion of genetic information that is freely available to identify polymorphisms that may determine drug response 2
![n Advances in molecular genetics and genotyping technologies during the last two decades have n Advances in molecular genetics and genotyping technologies during the last two decades have](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-3.jpg)
n Advances in molecular genetics and genotyping technologies during the last two decades have led to identification of many polymorphisms in phase I and phase II drug metabolising enzymes, drug targets, and in drug transporters 3
![Individual Variation in Response to Drugs How individuals in a population are expected to Individual Variation in Response to Drugs How individuals in a population are expected to](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-4.jpg)
Individual Variation in Response to Drugs How individuals in a population are expected to respond to a fixed dose of drug? n Inter-individual variability: - Some show less than usual response - Most show usual response - Others show more than usual response n 4
![Factors Determine Response to Drugs q Environmental (age, sex, race, concomitant diseases, diet, smoking, Factors Determine Response to Drugs q Environmental (age, sex, race, concomitant diseases, diet, smoking,](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-5.jpg)
Factors Determine Response to Drugs q Environmental (age, sex, race, concomitant diseases, diet, smoking, alcohol) q Genetic (polymorphisms drug metabolising enzymes, receptors, drug targets) 5
![Pharmacogenetics/Pharmacogeno mics Pharmacogenetics: is study of variation in drug response due to heredity & Pharmacogenetics/Pharmacogeno mics Pharmacogenetics: is study of variation in drug response due to heredity &](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-6.jpg)
Pharmacogenetics/Pharmacogeno mics Pharmacogenetics: is study of variation in drug response due to heredity & is used in relation to genes determining drug metabolism n Pharmacogenomics is a more general term; it refers to research area that comprises all genes in the human genome that may determine drug response n 6
![Benefits of Pharmacogenetics/Pharmacogenomi cs The concept “The right medicine to the right patient” is Benefits of Pharmacogenetics/Pharmacogenomi cs The concept “The right medicine to the right patient” is](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-7.jpg)
Benefits of Pharmacogenetics/Pharmacogenomi cs The concept “The right medicine to the right patient” is the basis of pharmacogenetics (personalised or individualised medicine) n Ultimate goals are to improve clinical therapeutic outcome by: - Increasing drug efficacy - Increasing safety of drugs e. g. reducing incidence of ADRs n 7
![Personalised or Individualised Medicine 8 Personalised or Individualised Medicine 8](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-8.jpg)
Personalised or Individualised Medicine 8
![Pharmacogenomic approach to personalized medicine. Drug therapy is chosen for each patient based on Pharmacogenomic approach to personalized medicine. Drug therapy is chosen for each patient based on](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-9.jpg)
Pharmacogenomic approach to personalized medicine. Drug therapy is chosen for each patient based on their particular genetic profile 9
![n Polymorphisms can occur in any gene that encode: - Drug metabolising enzymes - n Polymorphisms can occur in any gene that encode: - Drug metabolising enzymes -](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-10.jpg)
n Polymorphisms can occur in any gene that encode: - Drug metabolising enzymes - Drug transporters - Drug targets and receptors 10
![Genetic polymorphisms of drug metabolising enzyme genes n n n The majority of phase Genetic polymorphisms of drug metabolising enzyme genes n n n The majority of phase](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-11.jpg)
Genetic polymorphisms of drug metabolising enzyme genes n n n The majority of phase I and phase II drug metabolising enzymes are polymorphic The cytochrome P 450 (CYP) enzymes are the most important group of phase I enzymes Polymorphisms in cytochrome P 450 genes can cause enzyme products with abolished or increased enzyme activity 11
![Cytochrome P 450 enzymes n All genes that encode for families 1 -3 are Cytochrome P 450 enzymes n All genes that encode for families 1 -3 are](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-12.jpg)
Cytochrome P 450 enzymes n All genes that encode for families 1 -3 are polymorphic & their capacity to metabolise drugs depends on the functional importance and frequency of variant alleles 12
![CYP 450 Enzymes CYP 2 D 6 n CYP 2 C 9 n CYP CYP 450 Enzymes CYP 2 D 6 n CYP 2 C 9 n CYP](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-13.jpg)
CYP 450 Enzymes CYP 2 D 6 n CYP 2 C 9 n CYP 2 C 19 n 13
![CYP 2 D 6 n CYP 2 D 6 contributes to metabolism of large CYP 2 D 6 n CYP 2 D 6 contributes to metabolism of large](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-14.jpg)
CYP 2 D 6 n CYP 2 D 6 contributes to metabolism of large of medications about 25% of all drugs, including: n Antidepressants (TCAs, SSRIs) n Antiarrythmics n Analgesics 14
![CYP 2 D 6 Phenotypes - Poor metabolisers( PM): lack functional enzyme - Intermediate CYP 2 D 6 Phenotypes - Poor metabolisers( PM): lack functional enzyme - Intermediate](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-15.jpg)
CYP 2 D 6 Phenotypes - Poor metabolisers( PM): lack functional enzyme - Intermediate metabolisers (IM): carry two alleles that cause reduce activity - Extensive metabolisers ( EM): have two normal alleles - Ultra-rapid metabolisers (UM): multiple gene copies 15
![Poor metabolisers can experience adverse effects when treated with standard dose n Ultra-rapid metabolisers Poor metabolisers can experience adverse effects when treated with standard dose n Ultra-rapid metabolisers](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-16.jpg)
Poor metabolisers can experience adverse effects when treated with standard dose n Ultra-rapid metabolisers require high doses of drugs n 16
![Depression Tricyclic antidepressants are metabolised by CYP 2 D 6 n Disposition of nortriptyline Depression Tricyclic antidepressants are metabolised by CYP 2 D 6 n Disposition of nortriptyline](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-17.jpg)
Depression Tricyclic antidepressants are metabolised by CYP 2 D 6 n Disposition of nortriptyline is related to number of active CYP 2 D 6 alleles and n Dose required to obtain same plasma drug concentrations varies between subjects with different CYP 2 D 6 phenotypes - 17
![Ultra-rapid metabolisers needed a 10 fold larger dose of nortriptyline than poor metabolisers to Ultra-rapid metabolisers needed a 10 fold larger dose of nortriptyline than poor metabolisers to](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-18.jpg)
Ultra-rapid metabolisers needed a 10 fold larger dose of nortriptyline than poor metabolisers to achieve the same plasma concentration n Ultra-rapid metabolisers require 500 mg of doses compared to 50 mg in poor metabolisers n 18
![n n n Genetic polymorphisms of CYP 2 D 6 gene may be associated n n n Genetic polymorphisms of CYP 2 D 6 gene may be associated](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-19.jpg)
n n n Genetic polymorphisms of CYP 2 D 6 gene may be associated with ADRs and clinical response to antidepressants 30% of patients with ADRs to antidepressants were PMs High incidence of UMs among nonresponders (20%) 19
![CYP 2 C 9 n n CYP 2 C 9 metabolises a wide range CYP 2 C 9 n n CYP 2 C 9 metabolises a wide range](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-20.jpg)
CYP 2 C 9 n n CYP 2 C 9 metabolises a wide range of drugs Including drugs with narrow therapeutic indices such as S-warfarin, phenytoin and various non-steroidal antiinflammatory drugs (NSAIDs) including ibuprofen, diclofenac and celecoxib 20
![Warfarin and Bleeding n n Warfarin is one of the most widely prescribed oral Warfarin and Bleeding n n Warfarin is one of the most widely prescribed oral](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-21.jpg)
Warfarin and Bleeding n n Warfarin is one of the most widely prescribed oral anticoagulant drugs It is used for: Prophylaxis and treatment of venous thromboemolism n Treatment of deep vein thrombosis (DVT) n Atrial fibrillation (AF) n In patients with prosthetic heart valves n 21
![Warfarin and bleeding n n n The main complication of warfarin therapy is haemorrhage Warfarin and bleeding n n n The main complication of warfarin therapy is haemorrhage](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-22.jpg)
Warfarin and bleeding n n n The main complication of warfarin therapy is haemorrhage Genetic polymorphisms in CYP 2 C 9 gives rise to variants with altered enzymes activity Two allelic variants CYP 2 C 9*2 (Arg 144 Cys) and CYP 2 C 9*3 (Ile 359 Leu) show 12% and 5% of enzyme activity of the wild type CYP 2 C 9*1 allele, respectively, and are associated with decreased warfarin dose requirements & an increased risk of bleeding 22
![Peptic Ulcer: Proton pump inhibitors (PPIs) are used for treatment of gastric acid related Peptic Ulcer: Proton pump inhibitors (PPIs) are used for treatment of gastric acid related](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-23.jpg)
Peptic Ulcer: Proton pump inhibitors (PPIs) are used for treatment of gastric acid related diseases such as peptic ulcers, gastro-esophageal reflux disease (GERD) & in combination with antibiotics (amoxicillin & clarithromycin) for eradication of Helicobacter pylori (Hp) - CYP 2 C 19 metabolises several PPIs including omeprazole and lanzoprazole - Plasma concentrations of omeprazole, depend on patient’s CYP 2 C 19 phenotype - 23
![Ampli. Chip CYP 450 Array n The Ampli. Chip CYP 450 Test provides comprehensive Ampli. Chip CYP 450 Array n The Ampli. Chip CYP 450 Test provides comprehensive](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-24.jpg)
Ampli. Chip CYP 450 Array n The Ampli. Chip CYP 450 Test provides comprehensive detection of gene variations including deletions and duplications for the CYP 2 D 6 and CYP 2 C 19 genes 24
![Genetic polymorphisms of drug metabolising enzyme genes n n With respect to phase II Genetic polymorphisms of drug metabolising enzyme genes n n With respect to phase II](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-25.jpg)
Genetic polymorphisms of drug metabolising enzyme genes n n With respect to phase II enzymes, the most important polymorphisms occur in Nacetyltransferase-2 (NAT-2) and thiopurine methyltransferase (TPMT) NAT-2 is involved in the metabolism of isoniazid and sulphamethoxazole 25
![Acetylation - - Most individuals are either rapid or slow acetylators, but proportion varies Acetylation - - Most individuals are either rapid or slow acetylators, but proportion varies](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-26.jpg)
Acetylation - - Most individuals are either rapid or slow acetylators, but proportion varies between races The percentage of slow acetylators: § 90% in North African § 50% in Caucasian § 10% in Asian populations 26
![Thiopurine S-methyltransferase (TPMT) TPMT catalyzes methylation of thiopurine drugs such as 6 -mercaptopurine & Thiopurine S-methyltransferase (TPMT) TPMT catalyzes methylation of thiopurine drugs such as 6 -mercaptopurine &](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-27.jpg)
Thiopurine S-methyltransferase (TPMT) TPMT catalyzes methylation of thiopurine drugs such as 6 -mercaptopurine & azathioprine n These drugs are commonly used in treatment of acute lymphoblastic leukaemia (ALL), autoimmune diseases, inflammatory bowel diseases, in organ & tissue transplantation n Clinical testing for TPMT genetic polymorphisms is available n 27
![It has been shown that: - 90% of population exhibit high TPMT activity - It has been shown that: - 90% of population exhibit high TPMT activity -](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-28.jpg)
It has been shown that: - 90% of population exhibit high TPMT activity - 10% show intermediate activity - 0. 3% have low or absent enzyme activity n 28
![Genetic Polymorphisms in Drug Transporters n n Transporters are membrane proteins that play crucial Genetic Polymorphisms in Drug Transporters n n Transporters are membrane proteins that play crucial](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-29.jpg)
Genetic Polymorphisms in Drug Transporters n n Transporters are membrane proteins that play crucial role in absorption, distribution & elimination of drugs Genetic polymorphisms can occur in transport proteins & may contribute to interindividual variation in drug response MDR 1 (multi-drug resistant) Pglycoprotein-Digoxin Serotonin transporter-antidepressant response 29
![Genetic polymorphisms in Drug targets and receptors n n Drug target genes including those Genetic polymorphisms in Drug targets and receptors n n Drug target genes including those](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-30.jpg)
Genetic polymorphisms in Drug targets and receptors n n Drug target genes including those coding for receptors, ion channels and specific enzymes are subject to genetic polymorphisms B 2 -adrenergic receptor- B 2 agonist (salbutamol) Angiotensin converting enzyme (ACE)ACE inhibitors (lisinopril) Vitamin K epoxide reductase complex (VKORC)- Warfarin 30
![Practical Points Genetic is an important factor responsible for failure to therapy & occurrence Practical Points Genetic is an important factor responsible for failure to therapy & occurrence](http://slidetodoc.com/presentation_image_h2/1ca91be3cbe0f07e63c18d06e856dca6/image-31.jpg)
Practical Points Genetic is an important factor responsible for failure to therapy & occurrence of adverse drug reactions n The goal of PGx is to maximize efficacy & minimize toxicity, based on individual’s genetic composition n Individual variation in response to drug (some may benefit, other fail to respond to treatment, others may develop adverse effects) n 31
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