PERSONALIZED MEDICINE JAMES WEIS AND LILY CHAN Personalized

PERSONALIZED MEDICINE JAMES WEIS AND LILY CHAN

Personalized medicine takes into account individual genetic differences Traditionally, doctors used: Family history Socioeconomic circumstances Environmental factors Now: genomic/genetic testing proteomic profiling metabolomic analysis (study metabolites)

Effectiveness of drugs:

Danger of drugs: 6. 7% of patients in hospitals experience serious drug reactions

Old Paradigm:

New Paradigm:

Future Paradigm:

Personalized Medicine Today

The Plan

The Plan:

The Plan:

IT/CS Challenges Medical Challenges Biological Challenges Engineering Challenges

P. M.

Breather… (WHEW)

Gene Sequencing / Testing RFLP analysis SNPs (single nucleotide polymorphisms) More than 1. 4 million SNPs were identified in the initial sequencing of the human genome, with over 60, 000 of them in the coding region of genes (Evans and Mc. Leod 2003). (but even silent mutations can affect phenotype)

The accuracy of DTC genome-scan tests has been questioned, but Venter et al. found that the genotypes, or particular DNA bases observed, of an individual’s markers from 23 and. Me and Navigenics agreed more than 99. 7% of the time. Same for most other tests.

What the tests do disagree on, however, is the disease risk… Do predicted disease risks have any clinical validity? Genotype-phenotype correlation? Certain disease risks can be better predicted than others.

Pharmacogenomics DOES ONE SIZE REALLY FIT ALL?

Pharmacogenetics Study of genetic variation that gives rise to different responses to drugs It is estimated that genetics can account for 20 to 95 percent of variability in drug disposition and effects. Nongenetic factors include: age, organ function, concomitant therapy, drug interactions, and the nature of the disease.

Pharmacogenomics – Under the PM Umbrella Better medication choices 100, 000 Americans die annually and 2, 000+ are hospitalized due to adverse reactions to medications Predict individual reactions to dugs Safer dosing options More exact dosing, optimum result/side effect balance Improvements in drug development Exclude genetic variations from certain clinical trials, speeding up drug design time

Polygenic Determinants of Drug Response Differences in drug sensitivity and renal clearance Nine possible combinations of drug-metabolism and drugreceptor genotypes and the corresponding drug-response phenotypes. Each yields a different therapeutic index (efficacy: toxicity ratios) ranging from 13 (65 percent: 5 percent) to 0. 125 (10 percent: 80 percent). http: //content. nejm. org/cgi/content/full/348/6/538

Proteomic Profiling Relevant in the identification and predisposition to disease Josh’s presentation

Metabolomic Analysis First from urine analysis Networks of metabolite feedback pathways regulate gene and protein expression. Metabolites also can mediate signaling between organisms. Biomarkers of disease (diagnostics) The metabolome is therefore most predictive of phenotype (Fiehn 2002; Weckwerth 2003). However, “…an understanding of the resulting data is limited owing to a fundamental lack of biochemical and physiological knowledge about network organization…”

Metabolomic Analysis Metabolite target analysis focus on one specific metabolite Metabolic profiling group of metabolites, i. e those associated with a specific pathway. Extraction method (capillary electrophoresis, gas or liquid chromatography) specially designed for compounds in class to eliminate unwanted/ irrelevant metabolites. http: //swift. cmbi. ru. nl/euroschool/meta_seminar 1. pdf Metabolomics all metabolites, present in a cell or sample. Comprehensive analysis of entire metabolome under a given set of conditions. Metabolite fingerprinting the intention is not to identify each observed compound but to compare patterns or fingerprints of metabolites that change in response to disease or toxin exposure. Use statistical tools to look for major differences.

static dynamic

Applications of Personalized Medicine SOME BRIEF CASE STUDIES

Personalized medicine today yesterday Cytochrome P 450 genotyping test Enzyme group ‘cytochrome P 450’ (CYP 450 Many types of medications(including antidepressents, anticoagulants, proton pump inhibitors, etc) Determine dosing and effects of these drugs. Thiopurine methyltransferase test Thiopurine methyltransferase (TPMT) UGT 1 A 1 TA repeat genotype test Irinotecan (Camptosar) UGT 1 A 1 enzyme Dihydropyrimidine dehydrogenase test 5 -flourouracil (5 -FU) Dihydropyrimidine dehydrogenase enzyme Responsible for breaking down 5 -FU

Uses in Muscular Dystrophy: Becker and Duchenne MD – same family of disease; Duchenne’s more severe than Becker’s because generally the reading frame is preserved in BMD while it is not in DMD – death around age 20; BMD – life expectancy may be reduced, but some have a normal life span. Severity partially depends on mutation. Dystrophin is the largest known gene in the human body, located on the X chromosome. 79 exons ~15% caused by premature stop codons Phenotype-genotype correlation studies

Gentamicin treatment in DMD/BMD Aminoglycoside antibiotic synthesized by Micromonospora Works by binding the 30 S subunit (inhibition site) of the bacterial ribosome, interrupting protein synthesis (stop codon readthrough) Gentamicin treatment of Duchenne and Becker muscular dystrophy due to nonsense mutations. (Wagner et al 2001) Some success in mdx mouse model – suppressed truncation of protein and improved phenotype. Cons: highly nephrotoxic; can have psychiatric side effects.

Ataluren (PTC-124) and PRO 051 Mutation specific Both aim to restore reading frame: Ataluren does this through ribosomal stop codon readthrough PRO 051 does this through exon skipping (block splicing machinery) Duchenne Becker phenotype

Ataluren mechanism • Nonsense mutations result in a premature stop codon (UAG, UAA, or UGA) and cause a truncated protein. • Works best on UGA stop codon. Concept applicable to other diseases that also result from nonsense mutations, such as cystic fibrosis and nonsensemutation hemophilia A and B (nm. HA/B).

As mentioned before, only ~15% have nonsense mutations. Others have deletions, inversions, insertions, point mutations that do not result in a stop codon… Many specific sequences will be required for effectively treating the majority of patients with DMD. Will each specific sequence need to go through full testing required by the Food and Drug Administration (FDA)? Toxicity standards?

Analysis of Dystrophin Deletion Mutations Predicts Age of Cardiomyopathy Onset in Becker Muscular Dystrophy

Irinotecan Treatment for cancer that works by inhibiting topoisomerase 1, which prevents DNA from unwinding. Clinical studies have revealed significant associations between UGT 1 A 1*28 and irinotecan toxicity. A recommended strategy for irinotecan-dose adjustments based on individual genetic factors has not yet been fully established.

Selzentry™ (Pfizer) CCR 5 -tropic HIV treatment (CD 4 immune cells) Ineffective for CXCR 4 -tropic strains of HIV Trofile ™ assay to determine patients strain of HIV Detect virus that does not act through CCR 5 at levels as low as 0. 3% of a viral population Clinical trial patients selected with Trofile ™ assay

“The Food and Drug Administration, in a harbinger of likely future actions, has just approved a drugdiagnostic combination for AIDS patients who are running out of treatment options … the hallmark of personalized medicine. ” Edward Abrahams Personalized Medicine Coalition, 2007

The End OR IS IT JUST THE BEGINNING? . . .