Peroxidase lab What is peroxidase All organisms have

Peroxidase lab

What is peroxidase? § All organisms have enzymes (catalysts) called peroxidases that break down hydrogen peroxide into less harmful substance. Hydrogen Peroxide (H 2 O 2) is a pale blue liquid that appears clear when diluted. Hydrogen peroxide is a weak acid that is used as a bleaching agent and disinfectant.

Do Learn Connect §Do: Peroxidase Lab §Learn: I can identify the products of a reaction and classify reactions in terms of energy. §Connect: Properties and Patterns, Energy

Question: §Will chicken liver react with hydrogen peroxide and if so what products are formed?

Hypothesis § If chicken liver reacts with hydrogen peroxide, then I will observe _____ (chemical reaction clues) and ______ will be formed (products) because ______________________________ ______

Materials §Chicken Liver §Hydrogen Peroxide §Erlenmeyer Flask §Stopper §Toothpick §Candle

Procedure § Add Hydrogen Peroxide to flask. § Stopper the flask. § Record observations § Remove stopper from flask and insert toothpick into neck of flask. Do not drop it. § Record observations § Stopper flask. § Use the candle to ignite the toothpick, blow out the flame. § Remove stopper from flask and insert toothpick into neck of flask. Do not drop it.

Procedure § Add a small amount of chicken liver to the flask. § Stopper the flask. § Record observations. § After about 2 minutes, carefully feel the bottom of the flask. § Record Observations § Ignite the toothpick and blow out the flame. § Remove stopper and insert toothpick into the neck of the flask. Do not drop it. § Record observations. § Stopper the flask and repeat the last steps three more times.

Consider these questions: § Why did you make observations after each step? § What observations indicated that a chemical change took place? (Clues) § Classify the reaction as endothermic or exothermic using evidence. § What products were formed in this reaction? § Why is it important to replace the stopper quickly when it had been removed?

Products §The equation for the reaction is : 2 H 2 O 2 ____ and ____

CER (Conclusion) § Claim: The chicken liver did react with the hydrogen peroxide forming _______. (Products) § Evidence: During the chemical reaction, I observed ________. (Clues) § Reasoning: Provide your own reasoning connecting your products and clues to chemical reactions. Pre-Ap should also connect to ENERGY.