Perfused Bioreactor with MatrixEnabled Capillary Scaffold MECS Team

Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P. Wikswo A continuation from 2004 -2005 (Barnett, Garrett, Harvill, Mayer, Mc. Clintock)

Objective Design a tissue engineering scaffold and supporting microfluidic control system to guide the development of a network of capillary tubes in vitro.

Introduction l l Bioreactor Capillary network Matrix-Enabled Capillary Scaffold (MECS) Perfusion

Motivation l Simplified model for disease research l Human system l Control at many levels l Variety of uses http: //www. kids 4 research. org/mouse%20 in%20 hand%20 facing%20 left. jpg

Martins-Green a a

Bioreactor Design Supply Network Support Filter Collagen MECS Collagen Support Filter Supply Network

Overall Superstructure

Fractal pattern of vasculature

Matrix-Enabled Capillary Scaffold

Fabrication Strategy 1. Use two-layer photolithography to create mold of ‘egg crate’ design 2. 3. Use soft lithography to create a PDMS stamp Use stamp to deposit a sacrificial layer between two layers of collagen

Overall fluidics

Pumps and Lab. VIEW

Main Control Program Graphs p. H ranges PID coefficients p. H sensor settings Pump settings Histories of graph values

Main control program

Project Goals l l l Model a complete microvascular network Support cellular processes Allow evaluation of molecular mechanisms Provide environment for endothelial cell differentiation Facilitate formation of stable tubular structures Support a flow of perfusate

Collaborators Dmitry Markov, Ph. D Research advisor in microfabrication Lisa Mc. Cawley, Ph. D Research advisor in tissue culture Philip Samson Research advisor in microfabrication Jason Greene Graduate student working on related bioreactor projects Advice on interfacing pumps with computer Clean room manager Walter Georgescu Ron Resiserer

References l l l Martins-Green M, Li Q, Yao M. A New Generation Organ Culture Arising from Cross-Talk Between Primary Human Cell Types. Unpublished. Nanney, Lillian et al. Unpublished research. Tang MD et al. Fabrication of collagen gels that contain patterned micrometerscale cavities. Adv. Mater, 2004.