Perfused Bioreactor with MatrixEnabled Capillary Scaffold MECS Team
Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P. Wikswo
Motivation l Simplified model for disease research l Human system l Control at many levels l Variety of uses http: //www. kids 4 research. org/mouse%20 in%20 hand%20 facing%20 left. jpg
Martins-Green
Bioreactor Design Supply Network Support Filter Collagen MECS Collagen Support Filter Supply Network
Fractal pattern of vasculature
Matrix-Enabled Capillary Scaffold
Fluidic circuit design
Pumps and Lab. VIEW
Project Progress 1. Recruit team members and form group dynamics: l Bryan Gorman BME, Team leader, Control Systems and Microfabrication l Allyson Fry BME, Cell Culture Specialist l Jonathan Lin EE, Control Systems Specialist l Will Wong BME, Microfabrication Specialist
Project Progress 2. Microfabrication Progress Photolithography of “egg crate” mold close to perfection!
Project Progress 3. Tissue Culture Progress § Trained in tissue culturing and histological sectioning. § Began culturing cells. § Have grown transwell structures. 4. Control Systems Progress § Learning and executing Lab. VIEW programming. §Integration of pump flow control into Lab. VIEW §Design and testing of pump system.
Future tasks (for this semester) l Microfabrication: l l l Tissue culture: l l l Microfabrication of egg-crate. Begin PDMS scaffold to support egg-crate. Continue transwell cultures Label cells with immuno-fluorescence Control systems: l l l Implementation of pump system. Integrate p. H and O 2 sensors into Lab. VIEW Demonstration of closed-loop p. H control
Collaborators Dmitry Markov, Ph. D Research advisor in microfabrication Lisa Mc. Cawley, Ph. D Research advisor in tissue culture Philip Samson Research advisor in microfabrication Jason Greene Graduate student working on related bioreactor projects Advice on interfacing pumps with computer Clean room manager Walter Georgescu Ron Resiserer
References l l Martins-Green M, Li Q, Yao M. A New Generation Organ Culture Arising from Cross-Talk Between Primary Human Cell Types. Unpublished. Nanney, Lillian et al. Unpublished research.
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