PENTRA 60 C PENTRA 60 THROUGHPUT ANALYZES CBC

PENTRA 60 C +

PENTRA 60

THROUGHPUT ANALYZES CBC Mode 60/h 30 µl CBC + 5 Diff Mode 60/h 53 µl

PHYSICAL SPECIFICATIONS Weight: 35 Kg Height: 516 mm Depth: 444 mm Width: 481 mm

PHYSICAL SPECIFICATIONS Power supplies: from 100 Vac to 240 Vac 50 Hz to 60 Hz Power Consumption: Maximum 400 VA

Measurements and computation Impedance for WBC, PLT, RBC, BASO Photometry for Hgb Impedance, light scattering and cytometry for : LYM, MON, NEU, EOS, ALY, LIC. Computation from stored data that was directly measured for Hct, MCV, MCHC, RDW, MPV, Pct, PDW.

TECHNOLOGY Multi-Distribution Sequential System MDSS (ABX Patent) Double Hydrodynamic Sequential Syst. DHSS Counting Principle IMPEDANCE Permanent Rinse Flow PRF SOLENOID VALVES ABX PATENT

System technology MDSS Sample Dilution Process

Sample delivery - Aspiration of 53 µl of whole blood (5 Diff cycle) through the sampling needle - Whole blood split in 3 aliquots injected successively in 3 tangential flows of reagent

Sample Volumes

Step 1 : Sample Aspiration

Step 2 : Initial discard and rinse

Step 3 : First Dilution

Step 4 : WBC/BASO Dilution

Step 5 : DIFF Dilution

Step 6 : Probe Wash

Step 7 : DIFF - Stop Reaction

Step 8 : RBC/Plt Aspiration

Step 9 : RBC/Plt Dilution

Step 10 : Hgb Dilution

Final Dilutions First Dilution Diff chamber RBC/PLT chamber WBC/BASO chamber Hgb chamber 10µl + 1. 7 ml Dil - 42. 5µl 10µl = 1 ml Eosinofix After 12 sec 42. 5µL Dln + 10µl + 2 ml Alphalyse 2. 5 ml + 0. 4 ml + 1 ml Dil DILUTION 1/250 1/80 1/10000 1/200

Differential Dilution 1/80 25 µL Blood + 1000 µL Fix + 1000 µL Diluent = 1/80 • The Eosino. Fix reagent lyses the RBC, stabilizes the WBC in their native forms and stains the cells with a specific coloration, the Eosinophils are colored most intensely. • After 12 seconds reaction the solution is stabilized with Diluent and is then transferred to the flowcell for analysis.

General Characteristics of P 60 C+ User Interface Ø Pilots all the analysers’ cycles: analysis, regulation, maintenance and grinding cycles. Ø Calculates a result from the analyser measurements, pathology generation. Ø Follow-up system of the analyser reagent quantities. Ø Full view of result in colour Ø Management of a work list to predetermine analysis to be carried out.

General Characteristics of P 60 C+ User Interface Ø Stat analysis function. Ø Management of a barcode reader to identify samples. Ø Stocking of results, with consultation and research carried out by identification Ø Validation and re-run of the sample on request of the user. Ø Maintenance and regulation functions of analyser. Ø Parameters allowing the user to adapt the ASP 01 to its needs. Ø Managment of papers ensuring tracability of sensitive actions.

General Characteristics of P 60 C+ User Interface Ø QC: Quality Control. Ø Calibration of analyser Ø XB: patient quality control. Ø Repeatability. Ø Connection with a host computer system for reception of files and emission of results. Ø Printing.

CBC Result CBC

CBC/DIFF result