Pathology with Paper and Pencil Normal filter paper

Fabrication of Complex Structures on Paper Zigzag design to investigate mixing 13 11 12

Colorimetric Detection of Blood Glucose and Multiplexing Glucose detection process Paper microchannel Brown color

Paper-based Kits for Antimicrobial Susceptibility Assays Prototype: Indian patent file no: 201631023867 Working protocol:

Towards a Commercializable Device Functional block diagram of the med Colorimeter. Paper Based Micro-fluidic

Prototype Design Battery Paper Based Blood Sample Sensor Calibrated LED with Driver and Optical

Specification of the Prototype S. N. Parameters Values 1 Principle of detection Colorimetry 2

Acknowledgement Faculty Member Dr. Sudip Nag Department of E&ECE IIT Kharagpur, INDIA Researchers and

Acknowledgement Researchers and Students Dr. Shantimoy Kar Sujay Biswas Indian Institute of Technology Kharagpur,

Collaborative partners and funding Agencies Indian Institute of Technology Kharagpur, India - 721302

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Pathology with Paper and Pencil Normal filter paper [Ref: P. Mandal, R. Dey, S. Chakraborty, Lab Chip, 2012, 4026 -4028] Filter paper soaked in photoresist; prebaked Mask for UV exposure Paper channel Indian Institute of Technology Kharagpur, India - 721302

Fabrication of Complex Structures on Paper Zigzag design to investigate mixing 13 11 12 9 10 7 8 5 6 3 4 1 H-channel designed to investigate blood cell separation 2 Indian Institute of Technology Kharagpur, India - 721302

Colorimetric Detection of Blood Glucose and Multiplexing Glucose detection process Paper microchannel Brown color developed Glucose Reagent Blood Sample Reagents • Whatman grade 1 • Length = 20 mm Antibody • W = 3 mm, Φ = 7 (ABO) mm Calibration with standard glucose samples Separation + Reaction Plasma separated from RBC Calibration curve with Standard glucose Image Acquisition Blood sample to source pad Image Processing Result Display Multiplexing: Anti A-B Protein assay • Simultaneous detection of multiple analytes like glucose and protein etc. • Blood group Identification Anti O Glucose assay Indian Institute of Technology Kharagpur, India - 721302

Paper-based Kits for Antimicrobial Susceptibility Assays Prototype: Indian patent file no: 201631023867 Working protocol: Indian Institute of Technology Kharagpur, India - 721302 Microfluidics and Microscale Transport Processes Laboratory

Towards a Commercializable Device Functional block diagram of the med Colorimeter. Paper Based Micro-fluidic Sensor with Blood Sample LED based calibrated light source Calibrated photo detector Transimpedance amplifier + filters Analog to Digital Converter Microcontroller / Digital Signal Processo r Bluetooth Low Energy Transceiver A/Prof. Sudip Nag Indian Institute of Technology Kharagpur, India - 721302 Prof. Suman Chakraborty Smart Phone

Prototype Design Battery Paper Based Blood Sample Sensor Calibrated LED with Driver and Optical Detector Opaque Enclosure Bluetooth Low Energy Transceiver Indian Institute of Technology Kharagpur, India - 721302

Specification of the Prototype S. N. Parameters Values 1 Principle of detection Colorimetry 2 Wavelength/ illuminator 640 nm (red)/ LED 3 Intensity 13000 lux 4 Optical resolution of detector 0. 01 lux 5 RF carrier/ protocol 2. 4 GHz/ Bluetooth Low Energy 6 Digital resolution/ Speed of data transfer 23 bits/ 4 samples per second 7 App on smart phone Custom developed 8 Supply voltage/ Power 3. 0 V/ 50 m. W typical Indian Institute of Technology Kharagpur, India - 721302

Acknowledgement Faculty Member Dr. Sudip Nag Department of E&ECE IIT Kharagpur, INDIA Researchers and Students Dr. Shantimoy Kar (former Ph. D student) Sujay Biswas (current Ph. D student) Indian Institute of Technology Kharagpur, India - 721302

Acknowledgement Researchers and Students Dr. Shantimoy Kar Sujay Biswas Indian Institute of Technology Kharagpur, India - 721302

Collaborative partners and funding Agencies Indian Institute of Technology Kharagpur, India - 721302