Overview of Otolith Thin Slice Method SDAFS Catfish

  • Slides: 17
Download presentation
Overview of Otolith Thin Slice Method SDAFS Catfish Technical Committee 2014 Catfish Aging Workshop

Overview of Otolith Thin Slice Method SDAFS Catfish Technical Committee 2014 Catfish Aging Workshop

Procedures 1. Remove and dry 2. Set in epoxy resin 3. Thin slice using

Procedures 1. Remove and dry 2. Set in epoxy resin 3. Thin slice using a slow speed precision saw 4. Mount thin slice to a slide using Cytoseal™ 5. View – using a fiber optic light and dissecting scope

List of Items Used For Thin Slice Method -Slow speed precision saw (Isomet 1000)

List of Items Used For Thin Slice Method -Slow speed precision saw (Isomet 1000) and cutting fluid (Isocut Plus Cutting Fluid) -Epoxy resin and hardener (West Systems 105 epoxy resin and 207 hardener) -Ice cube trays -Disposable plastic cup and spoon -Microscope slides -Cytoseal™ 60 -Forceps -Warm water -Paper towels

2. Set otolith in epoxy resin -Mix resin and hardener in a disposable cup

2. Set otolith in epoxy resin -Mix resin and hardener in a disposable cup -Pour base layer of epoxy and let harden (~4 hours) -Set otoliths in the tray -Cover with a second layer of epoxy resin and let harden (~typically overnight)

2. Set otolith in epoxy resin Set otoliths in resin with posterior edge near

2. Set otolith in epoxy resin Set otoliths in resin with posterior edge near the edge of the epoxy resin Be Consistent! Set all otoliths with the same orientation to be able to make the same, consistent, cut on each otolith.

3. Thin slice w/slow speed precision saw

3. Thin slice w/slow speed precision saw

3. Thin slice w/slow speed precision saw Align the saw blade slightly to the

3. Thin slice w/slow speed precision saw Align the saw blade slightly to the right of the core of the otolith Zero the micrometer, set speed to 200 , lift chuck counter balance and push the blade button

3. Thin slice w/slow speed precision saw Cut off the posterior end of the

3. Thin slice w/slow speed precision saw Cut off the posterior end of the otolith Raise the counter balance, spin the micrometer wheel until. 025” appears on the digital readout

3. Thin slice w/slow speed precision saw

3. Thin slice w/slow speed precision saw

3. Thin slice w/slow speed precision saw Rinse thin slice in warm water and

3. Thin slice w/slow speed precision saw Rinse thin slice in warm water and dry Repeat process to take a second cut

4. Mount thin slice to a slide using Cytoseal™ Apply a drop of Cytoseal™

4. Mount thin slice to a slide using Cytoseal™ Apply a drop of Cytoseal™ to the slide, place thin slice on slide and cover with Cytoseal™ Remove air bubbles Allow to dry ~ 1 hour to view both sides of thin slice

5. View

5. View

5. View

5. View

5. View

5. View

5. View

5. View

Same technique can be used for many species Flathead catfish Black crappie Striped bass

Same technique can be used for many species Flathead catfish Black crappie Striped bass Largemouth bass Grass Carp? ?

Questions

Questions

Catfish otolith preparation for age interpretation One methodCatfish otolith preparation for age interpretation One method
Slice of Life Story Challenge March 2011 SliceSlice of Life Story Challenge March 2011 Slice
Rmske slice a sla Rmske slice Sa pouvajRmske slice a sla Rmske slice Sa pouvaj
2500 Series Slice IO 2500 Series Slice IO2500 Series Slice IO 2500 Series Slice IO
MSK SLICE Mgr Kateina Gajdoov VY32INOVACE15MSK SLICE AutorMSK SLICE Mgr Kateina Gajdoov VY32INOVACE15MSK SLICE Autor
SERIE CTI 2500 SLICE IO Serie 2500 SliceSERIE CTI 2500 SLICE IO Serie 2500 Slice
COOKING TERMS TO SLICE To Slice to cutCOOKING TERMS TO SLICE To Slice to cut
OSC Slice Discovery and Slice in Registration andOSC Slice Discovery and Slice in Registration and
Thin Slice 1 Software Engineering Laboratory Department ofThin Slice 1 Software Engineering Laboratory Department of
Thin Slice 1 Software Engineering Laboratory Department ofThin Slice 1 Software Engineering Laboratory Department of
Thin slice 1 Software Engineering Laboratory Department ofThin slice 1 Software Engineering Laboratory Department of
Overview Overview Overview Overview Overview Overview Overview RockOverview Overview Overview Overview Overview Overview Overview Rock
Thin Structure An Overview Support for Thin StructureThin Structure An Overview Support for Thin Structure
Otolith Shape Analysis using Wavelet Transfoms and CurvatureOtolith Shape Analysis using Wavelet Transfoms and Curvature
Comparison of results from Otolith and CWT markingComparison of results from Otolith and CWT marking
Otolith size trends in different bottom and depthOtolith size trends in different bottom and depth