Overview of Labeling Procedures Labeling q q Proper
Overview of Labeling Procedures
Labeling q q Proper and accurate labeling is one of the most important aspects of the study. Each participant selected for the survey will have their own individual set of labels. The label identifies the participant with a unique identifying sequence of numbers. This sequence of numbers matches with the barcode and provides key study information.
General Instructions q The questionnaires and labels will accompany the participant through the interview, specimen collection, and specimen processing process. § There will be small 0. 2 m. L PCR tube labels that each Laboratory Technician will already have (for ELISA). q Read the labels carefully. Be sure to match the participant to the label and to the correct item to be labeled.
Labeling Responsibilities q While in the field (Specimen Collection/Field Testing): § Interviewers are responsible for placing the “Questionnaire” label on the participant’s questionnaire at the start of the interview. • The Interviewer is also responsible for the HH labels § The Phlebotomist is responsible for labels related to specimen collection and field testing. § The Laboratory Technician is responsible for labels related to blood and urine processing. § The Pathologist is responsible for labels related to stool processing.
General Instructions q q Each participant will have their own individual set of labels. Each Household gets 6 labels: Nepal NMS Household Questionnaire IIIIIIIIII HH-0001 2016 Nepal NMS Household Salt Sample IIIIIIIIII HH-0001 2016 Nepal NMS Household Salt Bag IIIIIIIIII HH-0001 2016 Nepal NMS Household W Flour Sample IIIIIIIIII HH-0001 2016 Nepal NMS Household W Flour Bag IIIIIIIIII HH-0001 2016 Nepal NMS Household Extra IIIIIIIIII HH-0001 2016
General Instructions q Each Child 6 -59 mo gets 27 labels Interviewer Label Phlebotomist Labels. Specimen Collection Extra Labels- Can be used by Interviewer or Laboratory Technician Labels. Specimen Processing
General Instructions q Each Child 6 -9 yr gets 9 labels
General Instructions q Each Adolescent Boy 10 -19 yr gets 12 labels
General Instructions q Each Adolescent Girl 10 -19 yr gets 16 labels
General Instructions q Each Non-Pregnant Woman 15 -49 yr gets 30 labels
Points to Remember When Labeling in the Field q q Attach label to the vacutainer after blood collection. Attach label to the vacutainer in a left to right manner while holding the cap in the left hand such that the label may be read. Attach “Blue Top Vac” label to vacutainer in a left to right manner Blue Top Vacutainer
Interviewer- Questionnaire Label # Questionnaire q The Interviewer identifies the correct Label # and places the “Questionnaire” label onto the questionnaire at the start of the interview. q The Interviewer then gives the remaining labels to the Phlebotomist.
Specimen Collection Labels- Phlebotomist
Phlebotomist Specimen Control Form A Label # q The Phlebotomist identifies the correct Label # and places the “Specimen Ctrl A” label onto the “Specimen Control Form BA. q The remaining labels will be placed onto cryovials. Specimen Control Form A
Specimen Processing Labels- Laboratory Technician
Laboratory Technician. Specimen Control Form B Label # q The Lab Technician identifies the correct Label # and places the “Specimen Ctrl B” label onto the “Specimen Control Form B”. q The remaining labels will be placed onto cryovials. Specimen Control Form B
Lab Technician- PCR Tube Labels q. These labels are for 0. 2 m. L PCR tubes. 0008
Specimen Processing Labels- Pathologist
Pathologist Specimen Control Form C Label # q Pathologist identifies the correct Label # and places the “Specimen Ctrl C” label onto the “Specimen Control Form C”. q The remaining labels will be placed onto cryovials. Specimen Control Form C
Specimen Transfer- Blue Top Vacutainer 1 m. L serum 0001 100 u. L serum Blue Top Vacutainers will be collected from: § Children 6 -59 mo § Non-Pregnant Women 15 -49 yr Remaining serum
Specimen Transfer- Red Top Vacutainer 100 u. L serum 0001 Remaining serum Red Top Vacutainers will be collected from: § Adolescent Boys 10 -19 yr § Adolescent Girls 10 -19 yr § Pregnant Women 15 -49 yr
Specimen Transfer- Purple Top Vacutainer Whole blood lysate vial 1 Remaining plasma Whole blood lysate vial 2 PREPARE FIRST RBC Folate will be collected from: § Children 6 -59 mo § Adolescent Girls 10 -19 yr § Non-Pregnant Women 15 -49 yr PREPARE SECOND
Specimen Transfer- MRDR Purple Top Vacutainer 1 m. L plasma Remaining plasma MRDR will be collected from: § Children 6 -59 mo § Non-Pregnant Women 15 -49 yr
Sorting Specimens After Centrifugation q Label each specimen box with the specimen type Serum Vial 1 Serum Vial 2 RBC Fol Vial 1 RBC Fol Vial 2 Plasma Vial 1 0. 2 m. L PCR Tube MRDR Vial 1 MRDR Vial 2 Urine Vial 1 Urine Vial 2 Stool Vial 1 INCAP for serum Zinc an vitamin B 12 Back-up Specimen- Storage in NPHL Peking University Lab for RBC Folate Back-up Specimen- Storage in NPHL Ziploc Bag Germany for ELISA INCAP for MRDR & Retinol Back-up Specimen- Storage in NPHL INCAP for Urinary Iodine Back-up Specimen- Storage in NPHL AFRIMS for H. pylori
Questions?
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