ORF K pneumoniae TTT TTC TTA TTG CTT

ORFы в геноме K. pneumoniae

Генетический код TTT TTC TTA TTG CTT CTC CTA CTG ATT ATC ATA ATG GTT GTC GTA GTG F F L L L I I I M/ start V V TCT TCC TCA TCG CCT CCC CCA CCG ACT ACC ACA ACG GCT GCC GCA GCG S S P P T T A A TAT TAC TAA TAG CAT CAC CAA CAG AAT AAC AAA AAG GАT GАC GАA GАG Y Y stop H H Q Q N N K K D D E E TGT TGC TGA TGG CGT CGC CGA CGG AGT AGC AGA AGG GGT GGC GGA GGG C C stop W R R S S R R G G

Gene. Mark

Сигналы на границах генов dna. N gyr. A ser. S bof. A csf. B xpa. C met. S gca. D spo. VC fts. H pab. B rpl. J tuf. A rps. J rpo. A rpl. M ACATTATCCGTTAGGAGGATAAAAATG GTGATACTTCAGGGAGGTTTTTTAATG TCAATAAAAAAAGGAGTGTTTCGCATG CAAGCGAAGGAGATGAGAAGATTCATG GCTAACTGTACGGAGGTGGAGAAGATG ATAGACACAGGAGTCGATTATCTCATG ACATTCTGATTAGGAGGTTTCAAGATG AAAAGGGATATTGGAGGCCAATAAATG TATGTGACTAAGGGAGGATTCGCCATG GCTTACTGTGGGAGGAGGTAAGGAATG AAAGAAAATAGAGGAATGATACAAATG CAAGAATCTACAGGAGGTGTAACCATG AAAGCTCTTAAGGAGGATTTTAGAATG TGTAGGCGAAAAGGAGGGAAAATAATG CGTTTTGAAGGAGGGTTTTAAGTAATG AGATCATTTAGGAGGGGAAATTCAATG

… после выравнивания dna. N gyr. A ser. S bof. A csf. B xpa. C met. S gca. D spo. VC fts. H pab. B rpl. J tuf. A rps. J rpo. A rpl. M cons. num. ACATTATCCGTTAGGAGGATAAAAATG GTGATACTTCAGGGAGGTTTTTTAATG TCAATAAAAAAAGGAGTGTTTCGCATG CAAGCGAAGGAGATGAGAAGATTCATG GCTAACTGTACGGAGGTGGAGAAGATG ATAGACACAGGAGTCGATTATCTCATG ACATTCTGATTAGGAGGTTTCAAGATG AAAAGGGATATTGGAGGCCAATAAATG TATGTGACTAAGGGAGGATTCGCCATG GCTTACTGTGGGAGGAGGTAAGGAATG AAAGAAAATAGAGGAATGATACAAATG CAAGAATCTACAGGAGGTGTAACCATG AAAGCTCTTAAGGAGGATTTTAGAATG TGTAGGCGAAAAGGAGGGAAAATAATG CGTTTTGAAGGAGGGTTTTAAGTAATG AGATCATTTAGGAGGGGAAATTCAATG tacataaaggaggtttaaaaat 000000011111100001 5755779156663678679890

rbs. D в E. coli Eco AGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGC Eco ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTG

rbs. D в энтеробактериях Sty Sen Stm Eco Ype AGGGTTACACTGCGGC-CAGCGAAACGTTTCGCTAGTGGAGCAGAAAAATGAAGAAAGGC GGGGTTACACTGCGGC-CAGCGAAACGTTTCGCTAGTGGAGCAGAAAAATGAAGAAAGGC AGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAA-AAAAATGAAAAAAGGC TTTTCTAAACTCCTTGTTAGCGAAACGTTTCGCTCTTGGAGTA-GATCATGAAAAAAGGT ** ********* * * ***** Sty Sen Stm Eco Ype ACCGTACTCAACTCTGAAATCTCGTCGGTCATTTCCCGTCTGGGGCATACTGATACTCTG ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTG GTATTACTGAACGCTGATATTTCCGCGGTTATCTCCCGTCTGGGCCATACCGATCAGATT * ** ** ****** ***** *

rbs. D в энтеробактериях: ответ Sty Sen Stm Eco Ype AGGGTTACACTGCGGC-CAGCGAAACGTTTCGCTAGTGGAGCAGAAAAATGAAGAAAGGC GGGGTTACACTGCGGC-CAGCGAAACGTTTCGCTAGTGGAGCAGAAAAATGAAGAAAGGC AGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAA-AAAAATGAAAAAAGGC TTTTCTAAACTCCTTGTTAGCGAAACGTTTCGCTCTTGGAGTA-GATCATGAAAAAAGGT ** ********* * * ***** Sty Sen Stm Eco Ype ACCGTACTCAACTCTGAAATCTCGTCGGTCATTTCCCGTCTGGGGCATACTGATACTCTG ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTG GTATTACTGAACGCTGATATTTCCGCGGTTATCTCCCGTCTGGGCCATACCGATCAGATT * ** ** ****** ***** *

Паттерн нуклеотидных замен в белок-кодирующих областях: pdx. B в энтеробактериях Sty Stm Sen Eco Kpn Ype TCGCTCG--CAGCGGAAAGAGGATTACGCCCTTCGCCTGGAGGCTGTGCAGGGGC---GCCGGAGATGGGATGCATAATT TTGCCCG--TGCCAGACGGCAGATTATCTCCCTGACCTGGTGGTTGCCCAGGAGGAGGGCCGGAAATAGGTTGTATCATT ----CGG--TGGCGCAGTGCCTGATGGG-CCTCGCCCTGGAGGACGGTCTGGCAT---ATCAGCAAGGGGGTGCGTCATG TTGTTAGAACAGGGGAAAACGGTAAACAGTGTGGCATTAGATGTCGGTTATAGCT-----CCGCCTCTGCTTTTATCGCC * * * Sty Stm Sen Eco Kpn Ype AATTATCCTTTAAC-----CATAAATCTGAGCAATA-TATGCTTGGCGGCCAGATTATGGC--ACACTTGTCCGG AATTATCCTTTAAC----------CATAAATCTGAGCAATA-TATGCCTGGCGGCCAGATTATGGC--ACACTTGTCCGG ACGTATCCTTATAC-----CTGAAATCTTCGCAAG--TATGCCTGGCCGCGAGATTATGGC--ACACTTGTCCGG ATTCATCCTTTCGATATCGCGGTGCTGGAACCAGGTGATGAGTATGCCTGGCGGCCAGATTATGGC--ACACTTCCCCAG ATGTTTCAGCAAATAT----CGGGTACCA-CGCCTGAGCGTTTCCGGCGGGGCAATAGTGGCTTATACTAAGCCCC * ** * * *** **** * ** Sty Stm Sen Eco Kpn Ype TTAACTCTCGTT-CTCAAACAG------GTACGACAGTC--GTGAAAATTCTCGTTGATGAAAATATGCCTTACGCCCGC TTAACTCTCGT--CTCATACAG------GTAACACAAAC--GTGAAAATCCTTGTTGATGAAAATATGCCTTATGCCCGC TTAACTCTCGTT-CTCAGACAG------GTACTGAACT---GTGAAAATCCTCGTTGATGAAAATATGCCCGT CTGTTTTTCATCTGTATGGCAGTTCGCTGTCGGAGAGTAAAGTGAAAATTCTGGTTGATGAAAATATGCCGTACGCTGAG * * *** **** ** ********* ** ** 123123123123123123123

Белковое выравнивание (rib. D) Eco V_____QDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAGEPHAEVHA QD +M RAL LA +G +TT PNP VGCV VK+GEIVGEG+H +AG+PHAE A Hin MLEFSSQDCVFMQRALDLAAKGQYTTTPNPSVGCVLVKNGEIVGEGFHFKAGQPHAERVA Eco Hin GCGCGCCTGGAGGACTAA----G-----CCGTGCAGGAC-GAGTATTACATGGCGCGGGCGCTAA * * *** * ***** ** GAAAAATTAAAGGATTATGCTTGAATTTTCCTCACAAGATTGCGTATTT-ATGCAACGTGCCTTAG

Множественное выравнивание REC 06584 RECO 04717 RECS 04752 RTY 01088 RSY 05814 REO 01497 RYPK 00397 RYP 04048 RYE 04903 RVFI 01204 109 109 51 51 66 45 45 44 0 tttttatttcaggcaatcggggtgaat---------gtggcgcaggcggaagtgttgaat tttttatttcaggcaatcggggtgaat-----gtggcgcaggcggaagtgttgaat tagcgcctgttttgatttatggtgaacggggttaatgtggcgcaggcggaagtgttgaat atagcgcctgtttgatttcattgaattggggaaggcgtgtctacggcggaagtattgaat gccggcctgtgcagatctaatagttgggggaaaagtgtgtcgaccgcagcagtgataaac aaccggcctgtgcagatctcatagttggggaatagtgtgtcaaccgcagcagtgataaat. . . . tattattgatgagttttttatgtccagcatgatcgcagagcaaccaatggaa f l f q a i g v n = = = V A Q A E V L N * r l f * f m v n g v n V A Q A E V L N i a p v * f h * i g e g V S T A E V L N a g l c r s n s w g k s V S T A A V I N n r p v q i s * l g n s V S T A A V I N. . . i i d e f f m s s M I A E Q P M E

Сайты сплайсинга Donor sites gtgggatgatgtaagtattggggcggcccg tcaaaacaaggtaagaaatgaggtatgcct agctcccaaggtaggaggttgagtgttgtg agtggccaaggtatggtggaaattgc tggaaaaagcgtaagtcactctaattttat ctctcaaaaagtaagctttgtgagcatttc atcttcaagggtgagcatgtgtgttatgct tttcagaattgtaagagtacacattttaag gccagaaaaggtcagtactttcacac tacctcacaggtatgaattttctagttctt atctttcaaggtagagtatatgaatgttac atgtggattcgtaagtattcaacacattca aaaatatccagtaagcagttctgatgtttg ccaggagccggtgaggggctggtgggctct aatggatgaggtgggtacttagggcttctg atttcaaaaagttttccctggagaaa aatttgtagagtatccttgatttgacgaat cagacaatgggtaagtacatgcttgttccc gtctgttaaggtataccccatcacaa gttcaaaaaggttggtcacatgttcttgat attcggccaggtatgggtagtgtgctgaga acatatgcaggtaaacaacttaactcaaat aaagaggtgagagggtgttttaattt ccagctccaggtaagccatctggaaagagc gtcttaacaggtaaatgccaccctttcccc Acceptor sites gtttcttcttacatttctaggactcaacta ttcacgtttttgccttccaggagacagagc tttcaatattacccaggaccccaaat gtgttatttacatttttcaggaatggacaa tttttctgcttctccaacagctatactaaa ttgttgtgttcacagcatatatcgc tccgttgttttatttcccagaatgattcaa tggtttttcattgtttttagtggtgcaaaa tctaacttcatttcctccaggacaaatatc gttttgttggtgttttatagctggccaact acatgtgttctcatttttaggaagtgatag ctgttctcccttagcccaaagcag atgcctttcatttctattagctggaatctg ctgttattaaaatttgacaggagaagctga ttttttattcctacttccaggggactgctg tttgttgttgcttaactcagaaataa tacttaacatgatggtccagatataacaaa cttgtgtttttgatactcagacctggctat ttgatttattgattttctagattatttcag gtccttaatgtcctttgtaggtggttcttc gcattattctcaccttccaggctatcacta aatatctcttccctatttagatgtcatcga aaggatatttataattttaggctgatcctg ttttatcttttatattacaggttctgtaaa ttcatattcatttgttgcagaagtggaagc

Gen. Scan

MAGE-C 1

MAGEA 2

Genome. Scan=Gen. Scan+BLASTX

VISTA (human-dog-mouse)

HGB: m. RNAs, ESTs, repeats, conservation