Oral presentation Lab meeting Journal club Clear Qual

Oral presentation Lab meeting Journal club Clear Qual Thesis Presentation at meeting Interesting

Choice of a paper Goal: 1. On topic 2. Important and interesting to a large audience Localization AND (Cell [ta] OR Science [ta] OR Nature [ta]) Nature Methods

Introduction 2 -3 slides From general to particular

Introduction-Example 1

Introduction-Example 1 • Transcription is important in neural stem Significance? cells • SMRT=Nco. R 2 Question? is a nuclear co-repressor • Histones get methylated at lysine 27. What do I need to know? • There are demethylases that target these lysines Scheme/visuals? • SMRT -/- mice have defects in the cortex. Punch line?

Neural stem cells neural stem cells progenitors neuronal glial Development of the nervous system Regeneration of adult brain

Transcriptional regulation in neurogenesis? SMRT Program of target genes? Neuronal fate

Localization of molecules In situ hybridization of RNA: Target genes for SMRT Immunofluorescence: Role of SMRT on cell fate (neuronal versus glial markers)

Approaches 1 -2 slides 1 scheme is better than 1000 words Emphasize controls and limitations

Example-Figure 1 Title? Method used? Legend? Are all panels relevant? Is the Figure relevant? Conclusion?

SMRT normally prevents neuronal differentiation Method: In situ hybridization on brain sections at embryonic Day 12. 5 WT KO Emx 2 Mash 1 Dlx 1 Gad 1 Markers of differentiated neurons were increased in SMRT KO brains

Packaging Use Arial or Helvetica bold Black letters Yellow letters Use animation *not too much* Front 24 -44

Delivery Stand up Look at the audience Speak loudly + articulate Display enthusiasm Practice makes perfect