Nursing Journey com Microbiology Quiz Basic Lab Tests

© Nursing. Journey. com Microbiology Quiz Basic Lab Tests FYI, in this quiz there may be words used such as slant, but the photo doesn’t show the media as a slant. Therefore, just think about what you use in your lab, and relate that to what you are seeing here. Photos have been “borrowed” from the internet. If they are yours, please e-mail your URL so that I can give you credit. Fun 2 Care@yahoo. com Also, most information was taken from Alfred E. Benson’s Microbiology Applications, Short Version, 9 th Edition

© Nursing. Journey. com In a Gram stain, what are the positive and negative

© Nursing. Journey. com In a spore stain, what are the positive and negative

© Nursing. Journey. com In a acid-fast stain, what are the positive and negative

© Nursing. Journey. com What is this? What do the results reveal? http: //faculty.

© Nursing. Journey. com What is this? What do the results reveal? Motility Test Media - MTM Negative for Motility Growth is confined to the stab area. Positive for Motility Growth is radiating from the stab area, cloudiness is present http: //faculty. southwest. tn. edu/bdrake/Microbiology%20 Pictures. htm To test if the organism is motile, stab a depth of 75% of the media and incubate 24 -48 hours at the best growth temperature already decided.

© Nursing. Journey. com What is this? What do the results reveal? Gelatin Liquefaction - Nutrient Gelatin Negative result: Solid in ice bath or refrigerator after 5 minutes. Positive result: Liquid in ice bath or refrigerator after 5 minutes. Stab straight to bottom with an inoculating needle. Put in ice bath or refrigerator for 5 minutes, read results. If negative, reincubate up to 30 days.

© Nursing. Journey. com What is this? What do the results reveal? Phenylalanine Deaminase - Phenylalanine agar slant (PA) Positive result Green slant in 0 -10 minutes Streak slant with inoculator, After incubation time, use 5 drops of phenalalanine reagent. Negative result Yellow slant after 5 -10 minutes

© Nursing. Journey. com What is this? What do the results reveal? Glucose, Lactose or Mannitol Fermentation Phenol Red, Glucose, Lactose Mannitol Broth Positive Result: Negative Result: Yellow (positive for acid) Red-pink (negative for acid) Bubbles for gas) No bubbles (negative for gas) (positive Use Durham tube to test for gas, incubate 24 -48 hrs. Reincubate negative results up to 30 days.

© Nursing. Journey. com What is this? What do the results reveal? Methyl Red

© Nursing. Journey. com What is this? What do the results reveal? Urea hydrolysis

© Nursing. Journey. com What is this? What do the results reveal? Starch hydrolysis - Starch agar plate (SA) Positive Result Clear zone Negative Result Dark zone After incubating 24 -48 hrs, add grams iodine over growth.

© Nursing. Journey. com What is this? What do the results reveal? Citrate utilization - Simmon’s citrate agar slant (S/C) Negative Result Positive Result Uses citrate as sole carbon source Streak slant and stab butt, Incubate up to 30 days.

© Nursing. Journey. com What is this? What do the results reveal? Tryptophan hydrolysis (indole) - Tryptone broth Positive Result Red ring Negative Result Yellow ring 10 drops of Kovac’s reagent are added after a 24 -48 hour incubation.

© Nursing. Journey. com What is this? What do the results reveal? Casein hydrolysis

© Nursing. Journey. com What is this? What do the results reveal? http: //smccd.

© Nursing. Journey. com What is this? What do the results reveal? Litmus reactions - Litmus milk A = Alkaline C = Acid D = Litmus Reduction E = Peptonization (Another result may be coagulation, but is hard too distinguish in a photo. ) http: //smccd. net/accounts/case/biol 240/vlab. html 1 -5 days incubation time

© Nursing. Journey. com What is this? What do the results reveal? http: //biology.

© Nursing. Journey. com What is this? What do the results reveal? Hydrogen Sulfide Production - Kliger’s iron agar slant (KIA) Lactose Negative Hydrogen Sulfide Positive Black butt Red Slant http: //biology. uwsp. edu/faculty/TBarta/ Glucose Positive Yellow butt Streak slant for lactose, stab butt for glucose. Incubate 24 -48 hours. Save in refrigerator for new working stock. Lactose positive would be a yellow slant, and Glucose negative would be a red butt. If it is H 2 S+ negative, there will not be any black visible.

© Nursing. Journey. com What is this? What do the results reveal? http: //people.

© Nursing. Journey. com What is this? What do the results reveal? Voges-Proskauer Test, aka Acetoin - 1/2 MR-VP media Voges-Proskauer Negative Voges-Proskauer Positive Brown color Red color http: //people. uleth. ca/~selibl/Biol 3200/Bioch. Tests/ Add a dropperful of Barritt’s reagent A&B, after incubating for 5 days, and shake well for 2 hours.

© Nursing. Journey. com What is this? What do the results reveal? Oxidase Production - Nutrient agar plate (NA) Oxidase Negative Oxidase Positive No black colonies Black colonies within 30 minutes http: //biology. uwsp. edu/faculty/TBarta/ After incubation, tamp one oxidase disk on first quadrant

© Nursing. Journey. com What is this? What do the results reveal? Catalase Production - Nutrient agar slant (NA) Catalase Production Negative Catalase Production Positive No bubbles Bubbles http: //faculty. southwest. tn. edu/bdrake/Microbiology%20 Pictures. htm After a working stock has been incubated for 3 -5 days, add 3 drops of hydrogen peroxide. Make sure to use an OLD working stock for this test!

© Nursing. Journey. com What is this? What do the results reveal? http: //academic.

© Nursing. Journey. com What is this? What do the results reveal? Nitrate Reduction - Nitrate agar slant Negative for Nitrate Reduction Postive for Niatrate Reduction Clear color Red color http: //academic. mwsc. edu/jcbaker/images/nitrate%20 reduction. jpg Incubate, and then add 3 drops of Nitrate reagents A & B. If a negative result occurs, confirm the result with zinc dust. Opposite color results will occur: Clear positive, red negative for nitrate reduction.

© Nursing. Journey. com What is this? What do the results reveal? Fat Hydrolysis - Spirit Blue agar plate (SBA) Postive for Fat Hydrolysis Negative for Fat Hyrolysis Blue precipitate No precipitate http: //academic. pg. cc. md. us/~kroberts/lecture/web/exoenzymes/lipase. jpg Incubate for 24 -48 hours.

© Nursing. Journey. com Calculate the following dilution:

© Nursing. Journey. com Calculate the following dilution: The correct answer is: C. (Remember

© Nursing. Journey. com Calculate the following dilution: http: //smccd. net/accounts/case/biol 230/dilutions 2. html#

© Nursing. Journey. com Calculate the following dilution: 10 -2 10 -4 10 -5

© Nursing. Journey. com Calculate the following dilution: 10 -1 10 -2 10 -3

© Nursing. Journey. com Microbiology Quiz Basic Lab Tests Congratulations!! That’s all for this Microbiology quiz! Please remember, your instructor may require you to learn more, or less, of what is on this Power. Point. It’s your responsibility to learn what you will be tested on!! (Including knowing if any errors were made. ) Please report any errors to Fun 2 Care@Yahoo. com.