Nucleus and Mitosis VIBS 443602 Structure pancreatic acinar
Nucleus and Mitosis VIBS 443/602
Structure: pancreatic acinar cells Identification: nucleus, nucleolus, heterochromatin 1. Heterochromatin 2. Nucleus 3. Nucleolus
158 Pancreas
158 Pancreas
32409
32409 Nuclei with nucleoli
Structure: electron micrograph of hepatocyte (EM 5) Identification: nuclear envelope, inner and outer membrane, nuclear pores 1. Nuclear envelope 2. Heterochromatin 3. Euchromatin 4. Nuclear pore
EM 2 b cytoplasm nucleus
Structure: electron micrograph of stomach (EM 15) Identification: honeycomb nucleolus called nucleonema 1. Nucleus 2. Nucleonema
Structure: freeze etched of small lymphocyte (EM 6) Identification: fracture plane at one of the nuclear pores jumped from the outer to the inner membrane of the nuclear envelope 1. Inner nuclear membrane 2. Outer nuclear membrane 3. Nuclear pores
102 Mitosis anaphase
102 Mitosis - metaphase
102 Mitosis - anaphase
Three major events ORIENTATION in mitosis ALIGNMENT SEGREGATION
CHROMOSOME MOVEMENT Three contributions 3 Astral microtubules 1 2
Structure: intestinal absorptive cells Identification: nucleus, nucleolus 1. Nuclei 2. Nucleoli 152 201
201 Intestine crypt mitotic activity. 1. Brush border 2. Interphase nucleus 3. Anaphase 4. Artifact
152 duodenum
152 duodenum
152 duodenum
152 duodenum
152 duodenum
148
148
148 Ileum
32409
32409 Cells in mitosis
EM 10 f: Arteriolar wall; 30, 000 x. 1. Apical caveolae 2. Collagen 3. Dense bodies 4. Elastic fiber 5. Endothelial cell 6. Nuclear pore 7. RER
EM 19 c: Sertoli cell; 13, 300 x. 1. Golgi 2. Heterophagic vacuole 3. Lipid droplet 4. Microtubule 5. Nuclear pores (cross-section) 6. Sertoli cell 7. Synaptoneural complex
Colchicine treatment – chromosomes do not separate and NE forms around each chromosome due to breakdown of MT. 1. Multinucleated cell
Cytochalasin B treatment – chromosomes separate into two normal size nuclei, but the cytoplasm does not split in two Cytochalasin B treatment-breaks down microfilaments-nucleus activity but cells do not undergo cytokinesis 1. Binucleated cell 2. Nucleolus
EVENTS IN MITOSIS CYTOKINESIS
Nucleus and Mitosis
- Slides: 45