Nuclear Magnetic Resonance Spectroscopy Introduction to NMR Spectroscopy

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Nuclear Magnetic Resonance Spectroscopy Introduction to NMR Spectroscopy • Nuclear magnetic resonance spectroscopy is

Nuclear Magnetic Resonance Spectroscopy Introduction to NMR Spectroscopy • Nuclear magnetic resonance spectroscopy is a powerful analytical technique used to characterize the structure of organic molecules by identifying carbon-hydrogen frameworks within molecules. • Two common types of NMR spectroscopy are 1 H NMR is used to determine the type and number of H atoms in a molecule; while 13 C NMR is used to determine the type of carbon atoms in the molecule. • In NMR spectroscopy the EMR in radio waves regions (1 cm to 1 m wavelength range) are interacted and absorbed by molecule causing the nucleus to “spin flip” from one orientation to another. 1

Introduction to NMR Spectroscopy Some nuclei, such as 1 H, 13 C, 19 F,

Introduction to NMR Spectroscopy Some nuclei, such as 1 H, 13 C, 19 F, 31 P having nuclear spin. A spinning charge creates a magnetic moment, so these nuclei can be thought of as tiny magnets. If we place these nuclei in external magnetic field, they can line up with or against the applied field Bo by spinning clockwise or counter clockwise. Alignment with the magnetic field (called ) is of lower energy than against the magnetic field (called ). So the energy gap comes between these two alignments. m=– 1/2 ΔE=E-1/2 -E+1/2 = B 0 r(h/2 p) m=+1/2

This energy difference between the two spin states depends on the strength of the

This energy difference between the two spin states depends on the strength of the magnetic field (that the atom “feels”)

 • When an external energy source radio waves (h ) that matches the

• When an external energy source radio waves (h ) that matches the energy difference ( E) between these two states is applied, energy is absorbed, causing the nucleus to “spin flip” from one orientation to another. • Not all nuclei require the same amount of energy for the quantized spin ‘flip’ to take place. • The exact amount of energy required depends on chemical environment of the particular nucleus and the strength of external magnetic field applied. 4

The Chemical Shift of Different Protons NMR would not be very valuable if all

The Chemical Shift of Different Protons NMR would not be very valuable if all protons absorbed at the same frequency or of same energy radio waves. What makes it useful is that different protons usually appear at different chemical shifts ( . So, we can distinguish one kind of proton from another. Why do different protons appear at different ? There are several reasons, one of which is shielding and chemical environment of proton. The electrons in a bond shield the nuclei from the magnetic field. So, if there is more electron density around a proton, it sees a slightly lower magnetic field, less electron density means it sees a higher magnetic field: How do the electrons shield the magnetic field?

q How do the electrons shield the magnetic field? By moving. A moving charge

q How do the electrons shield the magnetic field? By moving. A moving charge creates a magnetic field, and the field created by the moving electrons Bi opposes the magnetic field of our NMR machine. It’s not a huge effect, but it’s enough to enable us to distinguish between different protons in our sample q We say that nuclei are shielded from the full applied magnetic field by the surrounding electrons because the secondary field diminishes the field at the nuclei.

q The electron density surrounding a given nucleus depends on the electronegativity of the

q The electron density surrounding a given nucleus depends on the electronegativity of the attached atoms. q The more electronegative the attached atoms, the less the electron density around the nucleus in question. q We say that nucleus is less shielded, or is deshielded by the electronegative atoms. q Deshielding effects are generally additive. That is, two highly electronegative atoms (2 Cl atoms, for example) would cause more deshielding than only 1 Cl atom. C and H are deshielded C and H are more deshielded

Chemical Shift q The relative position of absorption in the NMR spectrum is called

Chemical Shift q The relative position of absorption in the NMR spectrum is called chemical shift. It is a unitless number (actually a ratio, in which the units cancel), but we assign ‘units’ of ppm or (Greek letter delta) units. q For 1 H, the usual scale of NMR spectra is 0 to 10 (or 12) ppm (or ). q The usual 13 C scale goes from 0 to about 220 ppm. q The zero point is defined as the position of absorption of a standard, tetramethylsilane (TMS): q This standard has only one type of C and only one type of H and its peak Occurs at 0 ppm highly shielded.

How to calculate the Chemical Shift delta ppm Scale? For example, suppose CH 4

How to calculate the Chemical Shift delta ppm Scale? For example, suppose CH 4 molecule is placed in a magnetic field and radiated with EM radiation of just the right energy. In external magnet field of 7. 05 Tesla, it absorbs EM radiation of about 300 MHz (radio waves). So, if we bombard the molecule with 300 MHz radio waves, the protons will absorb that energy and we can measure that absorbance. In another magnet of 11. 75 Tesla, it absorbs EM radiation of about 500 MHz (stronger magnet means greater energy difference between the - and - state of the protons) But there’s a problem. If two researchers want to compare their data using magnets of different strengths, they have to adjust for that difference. It can be solved by using the delta scale.

The Chemical Shift (Also Called ) Scale Here’s how it works. First the frequencies

The Chemical Shift (Also Called ) Scale Here’s how it works. First the frequencies of the standard as well as of sample are measured and subtract the frequency of the sample from that of the standard. Then divide it by the frequency of the standard. This gives a number called the “chemical shift, ” also called delta , which does not depend on the magnetic field strength. Examples. Imagine that a magnet where the standard TMS absorbs at 300, 000 Hz (300 megahertz), and our sample absorbs at 300, 000, 300 Hz. The difference is 300 Hz, so we take 300/300, 000 = 1/1, 000 and call that 1 part per million (or 1 PPM). Now lets examine the sample in a stronger magnetic field where the reference TMS absorbs at 500, 000 Hz, or 500 megahertz. The frequency of our sample will increase proportionally, and will come at 500, 000, 500 Hz. The difference is now 500 Hz, but we divide by 500, 000 (500/500, 000 = 1/1, 000, = 1 PPM). It’s brilliant. Of course, we don’t do any of this, it’s all done automatically by the NMR machine. Even more brilliant.

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—The Spectrum • An NMR spectrum is a

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—The Spectrum • An NMR spectrum is a plot of the intensity of a peak against its chemical shift, measured in parts per million (ppm). 11

The Hard Part - Interpreting Spectra Four different features of a 1 H NMR

The Hard Part - Interpreting Spectra Four different features of a 1 H NMR spectrum provide information about a compound’s structure: a. b. c. d. Chemical shift data or position of signals Number of signals Intensity of signals Spin-spin splitting of signals.

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • In the vicinity

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • In the vicinity of the nucleus, the magnetic field generated by the circulating electron decreases the external magnetic field that the proton “feels”. • Since the protons experiences a lower magnetic field strength, it needs a lower frequency to achieve resonance. Lower frequency is to the right in an NMR spectrum, toward a lower chemical shift, so shielding shifts the absorption upfield. 13

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • The less shielded

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • The less shielded the nucleus becomes, the more of the applied magnetic field (B 0) it feels. • This deshielded nucleus experiences a higher magnetic field strength, to it needs a higher frequency to achieve resonance. • Higher frequency is to the left in an NMR spectrum, toward higher chemical shift—so deshielding shifts an absorption downfield. • Protons near electronegative atoms are deshielded, so they absorb downfield. 14

Nuclear Magnetic Resonance Spectroscopy Position of Signals Figure: How chemical shift is affected by

Nuclear Magnetic Resonance Spectroscopy Position of Signals Figure: How chemical shift is affected by electron density around a nucleus 15

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals Figure 14. 4 Shielding

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals Figure 14. 4 Shielding and deshielding effects 16

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals 17

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals 17

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Chemical Shift Values • The chemical shift of

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Chemical Shift Values • The chemical shift of a C—H bond increases with increasing alkyl substitution. 18

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • In a magnetic

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • In a magnetic field, the six electrons in benzene circulate around the ring creating a ring current. • The magnetic field induced by these moving electrons reinforces the applied magnetic field in the vicinity of the protons. • The protons thus feel a stronger magnetic field and a higher frequency is needed for resonance. Thus they are deshielded and absorb downfield. 19

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • In a magnetic

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals • In a magnetic field, the loosely held electrons of the double bond create a magnetic field that reinforces the applied field in the vicinity of the protons. • The protons now feel a stronger magnetic field, and require a higher frequency for resonance. Thus the protons are deshielded and the absorption is downfield. 20

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Chemical Shift Values 21

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Chemical Shift Values 21

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals Figure: Regions in the

Nuclear Magnetic Resonance Spectroscopy Chemical shift or Position of Signals Figure: Regions in the 1 H NMR spectrum 22

Nuclear Magnetic Resonance Spectroscopy b. Number of Signals • The number of NMR signals

Nuclear Magnetic Resonance Spectroscopy b. Number of Signals • The number of NMR signals equals to the number of different types of protons in a compound. • Protons in different environments give different NMR signals. • Equivalent protons give the same NMR signal. • To determine equivalent protons in cycloalkanes and alkenes, always draw all bonds to hydrogen. 23

Nuclear Magnetic Resonance Spectroscopy Number of Signals Figure: The number of 1 H NMR

Nuclear Magnetic Resonance Spectroscopy Number of Signals Figure: The number of 1 H NMR signals of some representative organic compounds 24

Nuclear Magnetic Resonance Spectroscopy Number of Signals • In comparing two H atoms on

Nuclear Magnetic Resonance Spectroscopy Number of Signals • In comparing two H atoms on a ring or double bond, two protons are equivalent only if they are cis (or trans) to the same groups. 25

Nuclear Magnetic Resonance Spectroscopy Number of Signals • Proton equivalency in cycloalkanes can be

Nuclear Magnetic Resonance Spectroscopy Number of Signals • Proton equivalency in cycloalkanes can be determined similarly. 26

Nuclear Magnetic Resonance Spectroscopy c. Intensity of Signals • The area under an NMR

Nuclear Magnetic Resonance Spectroscopy c. Intensity of Signals • The area under an NMR signal is proportional to the number of absorbing protons. • An NMR spectrometer automatically integrates the area under the peaks, and prints out a stepped curve (integral) on the spectrum. • The height of each step is proportional to the area under the peak, which in turn is proportional to the number of absorbing protons. • Modern NMR spectrometers automatically calculate and plot the value of each integral in arbitrary units. • The ratio of integrals to one another gives the ratio of absorbing protons in a spectrum. Note that this gives a ratio, and not the absolute number, of absorbing protons. 27

Nuclear Magnetic Resonance Spectroscopy Intensity of Signals 28

Nuclear Magnetic Resonance Spectroscopy Intensity of Signals 28

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Nuclear Magnetic Resonance Spectroscopy d. Spin-Spin Splitting • Consider the spectrum below: 30

Nuclear Magnetic Resonance Spectroscopy d. Spin-Spin Splitting • Consider the spectrum below: 30

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting • Spin-spin splitting occurs only between nonequivalent protons

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting • Spin-spin splitting occurs only between nonequivalent protons on the same carbon or adjacent carbons. Let us consider how the doublet due to the CH 2 group on Br. CH 2 CHBr 2 occurs: • When placed in an applied electric field, (B 0), the adjacent proton (CHBr 2) can be aligned with ( ) or against ( ) B 0. • Thus, the absorbing CH 2 protons feel two slightly different magnetic fields—one slightly larger than B 0, and one slightly smaller than B 0. • Since the absorbing protons feel two different magnetic fields, they absorb at two different frequencies in the NMR spectrum, thus splitting a single absorption into a 31 doublet.

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting The frequency difference, measured in Hz between two

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting The frequency difference, measured in Hz between two peaks of the doublet is called the coupling constant, J. 32

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Let us now consider how a triplet arises:

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Let us now consider how a triplet arises: • When placed in an applied magnetic field (B 0), the adjacent protons Ha and Hb can each be aligned with ( ) or against ( ) B 0. • Thus, the absorbing proton feels three slightly different magnetic fields—one slightly larger than B 0, one slightly smaller than B 0, and one the same strength as B 0. 33

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting • Because the absorbing proton feels three different

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting • Because the absorbing proton feels three different magnetic fields, it absorbs at three different frequencies in the NMR spectrum, thus splitting a single absorption into a triplet. • Because there are two different ways to align one proton with B 0, and one proton against B 0—that is, a b and a b— the middle peak of the triplet is twice as intense as the two outer peaks, making the ratio of the areas under the three peaks 1: 2: 1. • Two adjacent protons split an NMR signal into a triplet. • When two protons split each other, they are said to be coupled. • The spacing between peaks in a split NMR signal, 34 measured by the J value, is equal for coupled protons.

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting 35

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting 35

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Three general rules describe the splitting patterns commonly

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Three general rules describe the splitting patterns commonly seen in the 1 H NMR spectra of organic compounds. 1. Equivalent protons do not split each other’s signals. 2. A set of n nonequivalent protons splits the signal of a nearby proton into n + 1 peaks. 3. Splitting is observed for nonequivalent protons on the same carbon or adjacent carbons. If Ha and Hb are not equivalent, splitting is observed when: 36

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting is not generally observed between protons separated by

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting is not generally observed between protons separated by more than three bonds. 37

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Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Whenever two (or three) different sets of adjacent

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Whenever two (or three) different sets of adjacent protons are equivalent to each other, use the n + 1 rule to determine the splitting pattern. Figure The 1 H NMR spectrum of 2 -bromopropane, [(CH 3)2 CHBr] 39

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Now consider the spectrum of 1 -bromopropane. Since

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Now consider the spectrum of 1 -bromopropane. Since Ha and Hc are not equivalent to each other, we cannot merely add them together and use the n + 1 rule. Figure: The 1 H NMR spectrum of 1 -bromopropane, CH 3 CH 2 Br 40

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting When two sets of adjacent protons are different

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting When two sets of adjacent protons are different from each other (n protons on one adjacent carbon and m protons on the other), the number of peaks in an NMR signal = (n + 1)(m + 1). Figure 14. 8 A splitting diagram for the Hb protons in 1 -bromopropane 41

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting • Protons on carbon-carbon double bonds often give

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting • Protons on carbon-carbon double bonds often give characteristic splitting patterns. • A disubstituted double bond can have two geminal protons, two cis protons, or two trans protons. • When these protons are different, each proton splits the NMR signal of the other so that each proton appears as a doublet. • The magnitude of the coupling constant J for these doublets depends on the arrangement of hydrogen atoms. 42

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Figure: 1 H NMR spectra for the alkenyl

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Figure: 1 H NMR spectra for the alkenyl protons of (E)- and (Z)-3 -chloropropenoic acid 43

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Figure: The 1 H NMR spectrum of vinyl

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting Figure: The 1 H NMR spectrum of vinyl acetate (CH 2=CHOCOCH 3) 44

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting diagrams for the alkenyl protons in vinyl acetate

Nuclear Magnetic Resonance Spectroscopy Spin-Spin Splitting diagrams for the alkenyl protons in vinyl acetate are shown below. Note that each pattern is different in appearance because the magnitude of the coupling constants forming them is different. Figure: Splitting diagram for the alkenyl protons in vinyl acetate (CH 2=CHOCHOCH 3) 45

Nuclear Magnetic Resonance Spectroscopy OH Protons • Under usual conditions, an OH proton does

Nuclear Magnetic Resonance Spectroscopy OH Protons • Under usual conditions, an OH proton does not split the NMR signal of adjacent protons. • The signal due to an OH proton is not split by adjacent protons. Figure: The 1 H spectrum of ethanol (CH 3 CH 2 OH) 46

Nuclear Magnetic Resonance Spectroscopy OH Protons • Ethanol (CH 3 CH 2 OH) has

Nuclear Magnetic Resonance Spectroscopy OH Protons • Ethanol (CH 3 CH 2 OH) has three different types of protons, so there are three signals in its NMR spectrum. • The Ha signal is split by the two Hb protons into three peaks (a triplet). • The Hb signal is split only by the three Ha protons into four peaks, a quartet. The adjacent OH proton does not split the signal due to Hb. • Hc is a singlet because OH protons are not split by adjacent protons. • Protons on electronegative atoms rapidly exchange between molecules in the presence of trace amounts of acid or base. Thus, the CH 2 group of ethanol never “feels” the presence of the OH proton, because the OH proton is rapidly moving from one molecule to another. • This phenomenon usually occurs with NH and OH protons. 47

Nuclear Magnetic Resonance Spectroscopy Cyclohexane Conformers • Recall that cyclohexane conformers interconvert by ring

Nuclear Magnetic Resonance Spectroscopy Cyclohexane Conformers • Recall that cyclohexane conformers interconvert by ring flipping. • Because the ring flipping is very rapid at room temperature, an NMR spectrum records an average of all conformers that interconvert. • Thus, even though each cyclohexane carbon has two different types of hydrogens—one axial and one equatorial—the two chair forms of cyclohexane rapidly interconvert them, and an NMR spectrum shows a single signal for the average environment that it “sees”. 48

Nuclear Magnetic Resonance Spectroscopy Protons on Benzene Rings • Benzene has six equivalent deshielded

Nuclear Magnetic Resonance Spectroscopy Protons on Benzene Rings • Benzene has six equivalent deshielded protons and exhibits a single peak in its 1 H NMR spectrum at 7. 27 ppm. • Monosubstituted benzenes contain five deshielded protons that are no longer equivalent, and the appearance of these signals is highly variable, depending on the identity of Z. Figure: The 6. 5– 8 ppm region of the 1 H NMR spectrum of three benzene derivatives 49

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 50

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 50

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 51

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 51

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 52

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 52

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 53

Nuclear Magnetic Resonance Spectroscopy 1 H NMR—Structure Determination 53

Nuclear Magnetic Resonance Spectroscopy 13 C NMR 13 C Spectra are easier to analyze

Nuclear Magnetic Resonance Spectroscopy 13 C NMR 13 C Spectra are easier to analyze than 1 H spectra because the signals are not split. Each type of carbon atom appears as a single peak. 54