Nuclear Magnetic Resonance Spectroscopy an ideal tool for

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Nuclear Magnetic Resonance Spectroscopy -an ideal tool for Diagnosis & Drug Design www. assignmentpoint.

Nuclear Magnetic Resonance Spectroscopy -an ideal tool for Diagnosis & Drug Design www. assignmentpoint. com

CONTENTS v v v INTRODUCTION Ø BASIC PRINCIPLE Ø DIFFERENT TECHNIQUES APPLICATIONS OF NMR

CONTENTS v v v INTRODUCTION Ø BASIC PRINCIPLE Ø DIFFERENT TECHNIQUES APPLICATIONS OF NMR IN MEDICINE Ø CLINICAL APPLICATIONS IN DIAGNOSIS Ø DRUG DEVELOPMENT NMR Nobel prize laureates www. assignmentpoint. com

INTRODUCTION n n BASIC PRINCIPLE IN NMR Atomic nuclei – tiny magnets In external

INTRODUCTION n n BASIC PRINCIPLE IN NMR Atomic nuclei – tiny magnets In external magnetic field - allign or oppose If irradiated with EMR of proper frequency – Resonance √ α βo PARAMETERS § Chemical shifts § Spin lattice relaxation time www. assignmentpoint. com

DIFFERENT TECHNIQUES n n n Based on nuclei H 1 NMR C 13 NMR

DIFFERENT TECHNIQUES n n n Based on nuclei H 1 NMR C 13 NMR F 13 NMR & P 31 NMR N 15 NMR & O 17 NMR Ø Ø Ø CWNMR FTNMR 2 D_NMR &3 D_NMR COSY _CORRELATION SPECTROSCOPY MRI_MAGNETIC RESONANCE IMAGING ESR_ELECTRON SPIN RESONANCE SPECTROSCOPY www. assignmentpoint. com

APPLICATIONS OF NMR IN MEDICINE v CLINICAL APPLICATION OF PROTON IMAGING IN DIAGNOSIS BRAIN

APPLICATIONS OF NMR IN MEDICINE v CLINICAL APPLICATION OF PROTON IMAGING IN DIAGNOSIS BRAIN Distinguishing gray matter & white matter Ø Imaging posterior fossae, brain stem, spinal cord Ø Detect demyelinating lesions, tumors, hemorrhages, infarctions Ø www. assignmentpoint. com

ABDOMEN o o o Metabolic liver disease Measures liver iron over load in hemochromatosis

ABDOMEN o o o Metabolic liver disease Measures liver iron over load in hemochromatosis Focal areas of inflammation in chronic active hepatisis KIDNEYS o o Distinguishing renal cortex & medulla To evaluate transplanted kidney PELVIS o o Differentiates between Benign prostatic hyperplasia & prostatic carcinoma Detects bladder tumours www. assignmentpoint. com

MEDIASTINUM, HILUM, LUNG o o Assessing abnormalities Respiratory gating HEART o o o Tomographic

MEDIASTINUM, HILUM, LUNG o o Assessing abnormalities Respiratory gating HEART o o o Tomographic images of heart muscle, chambers, valvular structures ECHO-PLANAR TECHNIQUE Discrimination between infarcted, ischemic & normal myocardium BREAST o 3 D-NMR & single-slice planar imaging in detecting breast abnormalities www. assignmentpoint. com

MUSCULO SKELETAL SYSTEM Demonstrates Osteo myelitis, tumor metastasis in vertebral bodies & pelvic bones

MUSCULO SKELETAL SYSTEM Demonstrates Osteo myelitis, tumor metastasis in vertebral bodies & pelvic bones v Images of muscles, tendons, ligaments v BLOOD VESSELS & FLOW Ø Ø Atherosclerotic vascular disease Assess blood flow in major vessels. INVIVO SPECTROSCOPY Chemical shift phenomenon ü Diagnosis of rare disease to inborn errors. Mc Ardle’s syndrome ü INVIVO Analysis of Bone flouride content. www. assignmentpoint. com ü

PUTTING NMR IMAGING INTO PERSPECTIVE v v v Eliminates risk of x-radiation Excellent spatial

PUTTING NMR IMAGING INTO PERSPECTIVE v v v Eliminates risk of x-radiation Excellent spatial & contrast resolution Detecting diseases at earlier stages www. assignmentpoint. com

NMR IN PHARMACEUTICAL RESEARCH Ø Ø Ø Ø Leading technology for 3 -D structure

NMR IN PHARMACEUTICAL RESEARCH Ø Ø Ø Ø Leading technology for 3 -D structure determination of bio-macromolecules Studying protein structure SAR by NMR – Novel lead compounds Chemical shift mapping – Structural information on the binding modes and site positions Molecular dynamics, conformational analysis SHAPES NMR – SOLVE www. assignmentpoint. com

Structural genomics Identifying gene products in disease v Targets of drug design. v Development

Structural genomics Identifying gene products in disease v Targets of drug design. v Development of Novel drug delivery systems Enhancing HTS assays Thousands of compounds v Cryogenic NMR technology / cryoprobes v www. assignmentpoint. com

NMR Nobel Prize Laureates n n n The Nobel Prize in Physics 1943, Ohostern,

NMR Nobel Prize Laureates n n n The Nobel Prize in Physics 1943, Ohostern, USA – Discovery of magnetic moment of proton The Nobel Prizein Physics 1952 - Isidor. J. Rafi, USA – Discovery of new methods for nuclear magnetic precision The Nobel Prize in Chemistry 1991 – Felix Bloch, USA and Edward M. Purcell, USA – Methodology of high resolution NMR. The Nobel Prize in Chemistry 2002 – Kurt Wuthrich, Switzerland – Determining 3 -D Structure by NMR. The Nobel Prize in Medicine 2003 – Paul C. Lauterbur, USA and Peter Mans field, U. K. – www. assignmentpoint. com Discoveries concerning MRI.