Notes Nucleic Acids Continued DNA vs RNA PROTEIN

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Notes: Nucleic Acids Continued DNA vs. RNA

Notes: Nucleic Acids Continued DNA vs. RNA

PROTEIN SYNTHESIS 1. TRANSCRIPTION 2. TRANSLATION

PROTEIN SYNTHESIS 1. TRANSCRIPTION 2. TRANSLATION

Intro. to Protein Synthesis Watch the following 2 Amoeba Sisters Videos and complete the

Intro. to Protein Synthesis Watch the following 2 Amoeba Sisters Videos and complete the handouts. 1. “Why RNA is Just as Cool as DNA” (4: 43) 2. “Protein Synthesis and the Lean, Mean Ribosome Machines” (7: 08)

Step 1 = TRANSCRIPTION DNA m. RNA Step 2 = TRANSLATION m. RNA DNA

Step 1 = TRANSCRIPTION DNA m. RNA Step 2 = TRANSLATION m. RNA DNA Not on your sheet!

Step 1: Transcription • • Process of creating m. RNA from a DNA template(pattern)

Step 1: Transcription • • Process of creating m. RNA from a DNA template(pattern) [DNA m. RNA] Takes place in the nucleus TRANSCRIPTION PROCESS: 1. Enzymes unzip the DNA molecule 2. Free RNA nucleotides pair with matching DNA nucleotides • C pairs with G • G pairs with C T pairs with A A pairs with U 3. When base pairing is complete, the m. RNA molecule breaks away and DNA reforms. 4. The m. RNA leaves the nucleus and enters the cytoplasm

RNA • Function: Decodes information from DNA, then transports the message (code) to the

RNA • Function: Decodes information from DNA, then transports the message (code) to the ribosome for protein assembly.

Differences in DNA and RNA • DNA • RNA 1. Double stranded 2. Deoxyribose

Differences in DNA and RNA • DNA • RNA 1. Double stranded 2. Deoxyribose sugar 3. Contains Thymine as a nitrogen base 1. Single stranded 2. Ribose sugar 3. Contains Uracil as a Nitrogen base in place of Thymine

Types of RNA • m. RNA – carries coded DNA message out of nucleus

Types of RNA • m. RNA – carries coded DNA message out of nucleus • t. RNA – transports amino acids to the ribosome • r. RNA - involved with the ribosome

Essential Questions WHITE BOARD! 1. 2. 3. 4. What is Transcription? Where does transcription

Essential Questions WHITE BOARD! 1. 2. 3. 4. What is Transcription? Where does transcription occur? What is m. RNA? How is m. RNA different from DNA?

Translation m. RNA • Assembling of amino acids to form proteins • The CODON

Translation m. RNA • Assembling of amino acids to form proteins • The CODON sequence of m. RNA is translated into the amino acid sequence of a protein • Occurs in cytoplasm at ribosomes PROTEIN

Protein Synthesis SUMMARY • The building blocks of proteins are amino acids • DNA

Protein Synthesis SUMMARY • The building blocks of proteins are amino acids • DNA is the blueprint for the production of PROTEINS • 3 nitrogen BASES representing an amino acid is called a codon DNA RNA PROTEIN called: Central Dogma

Essential Questions WHITE BOARD! 1. 2. 3. 4. 5. What is translation? Where does

Essential Questions WHITE BOARD! 1. 2. 3. 4. 5. What is translation? Where does it occur? What is a codon? What are the monomers of Proteins? How does DNA code for protein?

Transcription & Translation Practice Now complete questions 2 -4 on your own

Transcription & Translation Practice Now complete questions 2 -4 on your own

You have to use an m. RNA codon chart!

You have to use an m. RNA codon chart!

m. RNA Amino Acid Sequence: CAU UCC AAA UGA ______________

m. RNA Amino Acid Sequence: CAU UCC AAA UGA ______________

- 16 on your own!UAA m. RNANow complete questions AUG 10 GUU UUC AGG

- 16 on your own!UAA m. RNANow complete questions AUG 10 GUU UUC AGG Check your completed work at the SSS Amino Acid Sequence: ______________

Protein Synthesis: DNA m. RNA Protein NOTES! Step 1 Trans. Cription DNA m. RNA

Protein Synthesis: DNA m. RNA Protein NOTES! Step 1 Trans. Cription DNA m. RNA Step 2 Trans. Lation m. RNA Protein

Transcription or Translation? How can you tell? -In the nucleus -Shows DNA - see

Transcription or Translation? How can you tell? -In the nucleus -Shows DNA - see m. RNA leaving

Transcription or Translation? How can you tell? -See a ribosome - no DNA

Transcription or Translation? How can you tell? -See a ribosome - no DNA

Transcription or Translation? How can you tell? -See a ribosome - no DNA

Transcription or Translation? How can you tell? -See a ribosome - no DNA

Transcription or Translation? How can you tell? -See a ribosome - no DNA

Transcription or Translation? How can you tell? -See a ribosome - no DNA

What is this process? ? ? DNA Replication How can you tell? -Only DNA

What is this process? ? ? DNA Replication How can you tell? -Only DNA is shown - 2 identical strands of DNA (semi-conservative replication) - no RNA (thymine only!)

POGIL Time! • Groups of 4 • Roles: MANAGER, READER, RECORDER, REPORTER (list names/roles

POGIL Time! • Groups of 4 • Roles: MANAGER, READER, RECORDER, REPORTER (list names/roles on sticky notes) • We will PAUSE at #8 (model 1 key question)

Let’s hear from a few of our REPORTERS: CONTINUE WORKING THROUGH THE END OF

Let’s hear from a few of our REPORTERS: CONTINUE WORKING THROUGH THE END OF THE POGIL

MUTATIONS • Recall, mutations are ERRORS in DNA. • They can occur during DNA

MUTATIONS • Recall, mutations are ERRORS in DNA. • They can occur during DNA Replication (Interphase) OR during cell division OR during protein synthesis. Amoeba Sisters: “Mutations: the Potential Power of a Small Change” Fill in your viewing sheet while you watch. We will go over the questions afterwards.

DNA: m. RNA: Amino Acids: GGA CCU Proline CAC GUG Valine CTC GAG Glutamic

DNA: m. RNA: Amino Acids: GGA CCU Proline CAC GUG Valine CTC GAG Glutamic Acid

Mutations Worksheet Original DNA Sequence: TACACCTTGGCGACGACT Transcription! m. RNA sequence __________ Translation! Amino Acid

Mutations Worksheet Original DNA Sequence: TACACCTTGGCGACGACT Transcription! m. RNA sequence __________ Translation! Amino Acid Sequence: Use the Codon Chart! __________

Mutations Worksheet – Try one More Mutated DNA Sequence #1: TACATCTTGGCGACGACT Transcription! m. RNA

Mutations Worksheet – Try one More Mutated DNA Sequence #1: TACATCTTGGCGACGACT Transcription! m. RNA sequence __________ Translation! Amino Acid Sequence: Use the Codon Chart! __________ Will there likely be effects? (on the protein) _____ What kind of mutation is this?

m. RNA AUG UAG AAC CGC UGA Will there likely be effects? What kind

m. RNA AUG UAG AAC CGC UGA Will there likely be effects? What kind of mutation? Amino Acid Sequence: ______________

Protein Synthesis Activity! An Analysis of Alien DNA

Protein Synthesis Activity! An Analysis of Alien DNA