Nextgenerationsequencingspectratyping reveals public Tcell receptor repertoires in pediatric

Next-generation-sequencing-spectratyping reveals public T-cell receptor repertoires in pediatric very severe aplastic anemia and identifies a β chain CDR 3 sequence associated with hepatitis-induced pathogenesis by Pina F. I. Krell, Susanne Reuther, Ute Fischer, Thomas Keller, Stephan Weber, Michael Gombert, Friedhelm R. Schuster, Corinna Asang, Polina Stepensky, Brigitte Strahm, Roland Meisel, Jens Stoye, and Arndt Borkhardt haematol Volume 98(9): 1388 -1396 September 4, 2013 © 2013 by Ferrata Storti Foundation

Characteristics of the patients enrolled in TCR repertoire profiling. Pina F. I. Krell et al. Haematologica 2013; 98: 1388 -1396 © 2013 by Ferrata Storti Foundation

Analysis of TCR diversity of healthy controls, v. SAA and h. BMT patients by conventional spectratyping and NGS-S. Pina F. I. Krell et al. Haematologica 2013; 98: 1388 -1396 © 2013 by Ferrata Storti Foundation

(A) Analysis of CDR 3 length polymorphism in the analyzed cohorts by NGS-S. Pina F. I. Krell et al. Haematologica 2013; 98: 1388 -1396 © 2013 by Ferrata Storti Foundation

(A) Analysis of CDR 3 length polymorphisms and TRBJ gene usage by NGS-S as an average for the respective cohort. Pina F. I. Krell et al. Haematologica 2013; 98: 1388 -1396 © 2013 by Ferrata Storti Foundation

CDR 3 sequences present exclusively in v. SAA patients. Pina F. I. Krell et al. Haematologica 2013; 98: 1388 -1396 © 2013 by Ferrata Storti Foundation

Overall and subgroup-specific average frequency of public TCR β chain sequences present in all SAA patients. Pina F. I. Krell et al. Haematologica 2013; 98: 1388 -1396 © 2013 by Ferrata Storti Foundation