New Scanner Developments Viktor Sebestyn Varga Ph D

New Scanner Developments Viktor Sebestyén Varga, Ph. D. Distributor meeting, February 24, 2014, Herceghalom

Overview • Scanners • New brightfield camera • New fluorescent camera • Objective changer for more microscopes • New software features

Scanners Pannoramic DESK • 1 slide • 1 objective • Brightfield only • Frozen section • Teleconsultation • Education

Scanners Pannoramic MIDI • 12 slides • Brightfield • Fluorescence • Objective changer • Manual camera changer • Research laboratory

Scanners Pannoramic SCAN • 150 slides • Brightfield • Fluorescence • Objective changer • Manual camera changer • Low throughput routine

Scanners Pannoramic 250 FLASH II • 250 slides • Brightfield • Fluorescence • Objective changer • Motorized camera changer • Continuous stage movement • High throughput routine

Scanner Software 1. 17 release

1. 17 FLASH II • Pannoramic 250 FLASH II support • BRIGHTFIELD • New 4 megapixel 1 chip CMOS camera • 130 frames / second scanning speed • New motor firmware for higher speed • Faster scanning • Faster slide loading

1. 17 FLASH II 15 mm x 15 mm area: 51 slides / hour @ 26 x (20 x + 1. 0 x) 33 slides / hour @ 40 x 27 slides / hour @ 52 x (20 x + 1. 6 x) (40 x + 1. 0 x)

1. 17 FLASH II • 40 x scanning became available in FLASH mode 27 slides / hour @ 52 x (40 x + 1. 0 x) • 40 x + 1. 6 x is not supported due to low light intensity

1. 17 Stop-and-go for FLASH I and II • If there is contamination on the bottom of the slide this mode provides better results • Scanning speed is lower

1. 17 4 megapixel camera • The new 4 megapixel camera is replacing the CIS 3 CCD camera • Recommended configuration is 20 x + 1. 6 x camera adapter • DESK • MIDI • SCAN

1. 17 New brightfield focus algorithm • New sample detection • on the preview image • on the focused field-of-views • More accurate focus point layout • Large focus changes in the sample are scanned separately

1. 17 Magazine unloading • The last magazine is not unloaded automatically • Easier rescan of slides

Scanner Software 1. 18 release

1. 18 Motorized objective changer • MIDI • SCAN

1. 18 PCO 4. 2 • New scientific CMOS camera PCO. edge 4. 2 • HIGHER QUANTUM EFFICIENCY • SHORTER EXPOSURE TIME • Same as Zeiss’

1. 18 PCO 4. 2 Resolution Quantum efficiency Noise Dynamic range We will ship it in BLACK PCO. edge 5. 5 2560 x 2160 PCO. edge 4. 2 2048 x 2048 < 60% > 70% 1. 1 electrons 27 000 : 1 0. 9 electrons 33 000 : 1

1. 18 PCO 4. 2 • Previously we used only the center 2. 56 (1600 x 1600 pixels) megapixels of the 5. 5 megapixel chip • Now we use the full 4. 2 megapixel of the new chip • With the software upgrade old cameras can use 3. 7 megapixels

1. 18 JPEG XR • New image codec for 16 bit images • JPEG XR is FASTER • JPEG 2000 is still supported for backward compatibility but not recommended

1. 18 Fluorescent speed improvement • Shorter exposure time • Larger chip area • Faster compression • 1. 92 times faster! • 1. 17: PCO. edge 5. 5, 2. 56 megapixel, 3 channels, 100 ms exposure each channel, JPEG 2000 80% • 1. 18: PCO. edge 4. 2, 4. 2 megapixel, 3 channels, 65 ms exposure each channel, JPEG XR 80% • 4: 48 minutes vs. 2: 30 minutes

1. 18 Fluorescent speed improvement • Shorter exposure time • Larger chip area • Faster compression • 2. 66 times faster! • 1. 17: PCO. edge 5. 5, 2. 56 megapixel, 3 channels, 100 ms exposure each channel, JPEG 2000 100% • 1. 18: PCO. edge 4. 2, 4. 2 megapixel, 3 channels, 65 ms exposure each channel, JPEG XR 100% • 6: 58 minutes vs. 2: 37 minutes

1. 18 Fluorescent speed improvement • Previous PCO. edge 5. 5 • Larger area used • Faster compression • 1. 56 times faster! • 1. 17: PCO. edge 5. 5, 2. 56 megapixel, 3 channels, 100 ms exposure each channel, JPEG 2000 80% • 1. 18: PCO. edge 5. 5, 3. 7 megapixel, 3 channels, 100 ms exposure each channel, JPEG XR 80% • 4: 48 minutes vs. 3: 05 minutes

1. 18 Fluorescent speed improvement • Previous PCO. edge 5. 5 • Larger area used • Faster compression • 2. 14 times faster! • 1. 17: PCO. edge 5. 5, 2. 56 megapixel, 3 channels, 100 ms exposure each channel, JPEG 2000 100% • 1. 18: PCO. edge 5. 5, 3. 7 megapixel, 3 channels, 100 ms exposure each channel, JPEG XR 100% • 6: 58 minutes vs. 3: 15 minutes

1. 18 Messenger E-mail can be sent for events • Error 2014. 02. 06. 12: 17: 51 - Slide scan has failed! There is not enough disk space to store the slide! • Warning • Last magazine • Scan is finished 2014. 02. 21. 2: 28: 09 Automatic scan finished. • Scan report 2014. 02. 20. 19: 27: 45 - 2014. 02. 20. 19: 29: 59 Magazine: 1 position: 5. Slide scan finished. Name: 1 M 05.

1. 18 Messenger

1. 18 Messenger

1. 18 Z difference • The objective focus point is different at different wave lengths • So far only one Z difference setting was available • Now the 2 objectives can have different settings

Competitors

Competitors in brightfield European Congress of Pathology, 2013 September, Lisbon Same slide, time includes focusing Pannoramic 250 FLASH II focus distance 15 1: 45 @ 40 x (objective 20 x, NA 0. 80 + 1. 6 x) Hamamatsu XR interpolated focus 2: 17 @ 40 x (objective: 20 x, NA 0. 75) GE Omnyx every field of view is focused 4: 11 @ 25 x „Digital 40 x” (objective: 20 x, NA 0. 75)

Competitors in brightfield United States & Canadian Academy of Pathology, 2013 March, Baltimore Same slide, time includes focusing Pannoramic 250 FLASH II focus distance 15 1: 45 @ 40 x (objective 20 x, NA 0. 80 + 1. 6 x) Hamamatsu XR Dynamic Pre-Focusing 2: 25 @ 40 x (objective: 20 x, NA 0. 75) Ventana i. Scan HT high throughput mode, less focus points 2: 10 @ 20 x (objective: unpublished)

Competitors in brightfield Brochures, 15 mm x 15 mm area / slide Pannoramic 250 FLASH II 33 slides / hour @ 40 x (objective 20 x, NA 0. 80 + 1. 6 x) includes, slide handling, preview, etc. Aperio AT 2 20 slides / hour @ 40 x (objective 20 x, NA 0. 75) includes, slide handling, preview, etc.

Competitors in fluorescence 3 channels, 20 ms / 100 ms exposure times, 5 mm x 5 mm, 306 field of views, 9 Z-stack layers. Pannoramic 250 FLASH II, 1. 18, PCO. edge 4. 2, quad band, Lumencor, JPEG XR 27 minutes scanning + 15 minutes software flat field correction Zeiss Axio. Scan Z 1, Hamamatsu ORCA-Flash 4. 0, single band, HXP 120, JPEG XR 45 minutes scanning + bad flat field correction + bad stitching

Thank you for your attention! viktor. varga@3 dhistech. com