New Oslo PSC biobank New Oslo PSC biobank

New Oslo PSC biobank

New Oslo PSC biobank § § § § Broad consent – covers all clinical and research biobanking Integrated storage of clinical data and biological material DNA, RNA (blood), serum, plasma, cell-isolates Biliary eptihelial cells (brushings), bile, intestinal biopsies Fresh tissue at liver explantation, cancer tissue at resection Standardized and written protocols all steps (SOP) Regular QC of stored material Barcode-based inventory system (-80°C/liquid nitrogen)

Physical storage system

Database integration

Biobanking - background Norsk senter for PSC

Biobanks ? § Population banks § Biomarkers of susceptibility and population identity, and their operational substrate is germinal-line DNA from a huge number of healthy donors, representative of a concrete country/region or ethnic cohort § Disease-oriented banks for epidemiology § Biomarkers of exposure, using a huge number of samples, usually following a healthy exposed cohort/ case–control design studying germinal-line DNA or serum markers and a great amount of specifically designed and collected data. § Disease-oriented general biobanks (i. e. tumour banks) § Biomarkers of disease through prospective and/or retrospective collections of tumour and no-tumour samples and their derivates (DNA/RNA/ proteins), usually associated to clinical data and sometimes associated to clinical trials. § The amount of clinical data linked to the sample determinate the availability and biological value of the sample Norsk senter for PSC

Biobanking in PSC – why and how ? §Ethiopatogenesis §Cholangiocancer §Serum/plasma samples §Tumor material §DNA-samples §Dysplasia cytology §Liver biopsies/ explanted liver §Bile duct cytology/biopsies §Colon biopsies: IBD+/- §Disease progression §Liver biopsies §Bile duct cytology/biopsies Norsk senter for PSC

Biobanking in PSC: Discussion § § Controversy: Competition vs. collaboration Types of material? § Minimal level Standardization (SOP) § study group website w/protocols? Material exchange conditions study group MTA ? Study proposals § centers § §