NEW EMPLOYEE SAFETY TRAINING ENVIRONMENTAL HEALTH SAFETY SECTION

NEW EMPLOYEE SAFETY TRAINING ENVIRONMENTAL HEALTH & SAFETY SECTION 2

AGENDA Section 2 • Laboratory Safety • Animal Safety • Radiation Safety • Biosafety • Exposure incidents • Laboratory Safety Training Requirements

LABORATORY SAFETY • Types of Hazards • Identifying Chemical Hazards • Right to Know/ Globally Harmonized System • Protective Measures • Hazardous Waste • Spill Procedure • Air Monitoring

LABORATORY HAZARDS PHYSICAL HAZARDS HEALTH HAZARDS w. Compressed gases w. Carcinogens w. Explosive materials w. Toxic or highly toxic agents w. Flammable liquids or solids w. Reproductive toxins w. Organic peroxides w. Irritants (cause irreversible w. Oxidizers w. Pyrophoric materials (ignites spontaneously in air) w. Unstable materials (reactive) w. Water reactive materials inflammatory effect on living tissue) w. Sensitizers (allergic reactions in normal tissue after repeated exposure)

RECOGNIZING HAZARDS

LABELING OF CHEMICALS (GHS) All containers must be labeled Regard unlabeled containers as hazardous Do not remove/deface labels § Manufacturers labels need to have: § Identity of chemical § Name and address of manufacturer § Appropriate hazard warning Under the new GHS containers additionally need: § § Signal Words Hazard Statements Precautionary Statements Pictograms § Laboratory Solutions: § Need to be labeled with the chemical names of all the constituents Do NOT use chemical formulas when labeling solutions

SAFETY DATA SHEETS • • Available for every hazardous chemical or substance Detailed information sheet prepared by manufacturer or importer • Provides information on: ® ® A material’s chemical make -up and properties The level of protective gear you need to wear to work safely with the material The preplanning needed for safely handling spills, fires, and day-to-day operations How to respond to accidents Electronic access to SDSs is available on the electronic chemical environmental management site (CEMS). No username/password is required to view SDS.

PROTECTIVE MEASURES Product Substitution Engineering Controls Safe Work Practices PPE

PRODUCT SUBSTITUTION What are the substitutes for hazardous chemicals? • Mercury containing chemicals & instruments Mercury free bleach and assay kits • Use alcohol thermometers Ethidium Bromide/ Acrylamide Gels • • • SYBR® Safe • Precast Acrylamide Gels Sodium Azide • Kathon CG/ICP Preservatives • Formaldehyde/ Formalin Ordering smaller quantities of higher hazard chemicals

ENGINEERING CONTROLS The primary means of protection Chemical Fume Hood Biosafey Cabinet Centrifuge Safety Cups Safer medical devices Shielding Hand washing Facility Some engineering controls require annual recertification (e. g. BSCs, VBSEs, fume hoods)

SAFE WORK PRACTICES • Follow standard operating procedures • Understand hazards of the materials you are working with • Consult PI prior to scaling up any experiments • Do not leave laboratory procedures unattended • • Practice good housekeeping • Mark peroxidizable chemicals with received and opened dates and dispose of prior to expiration date • Avoid keeping “legacy” and old containers Store incompatibles separately Texas Tech Lab Explosion Watch Video! Please click on the link to view this video

PERSONAL PROTECTIVE EQUIPMENT Additional means of protection • Latex (bio only) or Nitrile (bio or chemical) exam gloves (double gloves recommended) • Safety glasses with side shields/ goggles/face shields • Respiratory protection • Lab coats, uniforms • Tyvek gowns • Sleeves Watch Video! Please click on the link to view this video

HAZARDOUS WASTE PROCEDURE All waste is collected in the lab’s Satellite Accumulation Area (SAA)

CHEMICAL SPILL PROCEDURE • Contact Operational Engineers at 508 -856 -3292 • Secure the area • Alert others • If injured, go to Employee Health or Emergency Room

AIR MONITORING In the occurrence of a chemical spill, EH&S may monitor chemical concentrations in the air VOC Monitor Ammonia Meters Drager Tubes 4 gas meter

ANIMAL SAFETY • • PPE Handling & Husbandry Zoonoses Allergies Anesthetic Gases Controlled Substances Euthanasia

PERSONAL PROTECTIVE EQUIPMENT (CONTINUED) • Scrub suits/ jump suits • Safety glasses/ face shield • Mask/respirators • Hair covers • Shoe covers • Gloves

HUSBANDRY AND HANDLING Husbandry is the process of providing care for an animal • Examples: providing food, shelter, water Handle animals in manner prescribed for the species to prevent the following: • • • Splash hazards Contact with bodily fluids Bites/Scratches

ZOONOSES & ALLERGIES Zoonoses are diseases that can be transmitted from animals to humans • Causative agents include: • • Bacteria Viruses (Herpes B Virus) Parasites (Toxoplasma) Fungi (Ringworm) Allergies are immune responses by the body to a substance • • • Some people develop a respiratory allergy from working with animals Usually develop within the first 3 years Most common allergy is rhinotracheitis

ANESTHETIC GASES • Waste gases should be properly scavenged • If working outside anesthetic machine use a chemical fume hood or Biological Safety Cabinet if it is 100% exhaust • Don’t use Ether • Vaporizer should be calibrated annually on machines that use gas anesthetics

CONTROLLED SUBSTANCES In order to work with controlled substances, a researcher must have (or be listed as a registered user) on a controlled substance Researcher Registration • DEA regulates V Schedules of controlled substance • Schedule I: most addictive and abused • • • i. e. Heroine, Marijuana Schedule V: not often abused, minimal/no addictive properties DPH regulates all prescriptions drugs as schedule VI Commonly used Controlled Substances • Ketamine (III), Buprenorphine (III) Controlled substances must be kept in a substantially constructed cabinet, and perpetual inventories of drug usage must be maintained

EUTHANASIA The intentional causing of death to an animal • Painless • Humane & Respectful • “Double Kill” The method of euthanasia will be dependent on species in question and should be performed by trained personnel Avoid • Self injection • Exposure to gases • Exposure to Bodily Fluids

RADIATION SAFETY Additional training through Radiation Safety is required for work with radioactive isotopes Ionizing radiation passes through matter and can cause some of its atoms to become electrically charged, or ionized. In living tissues, the ions caused by such radiation can affect normal biological processes Ionizing radiation comes in several different forms: • Alpha particles - are positively charged particles. They are easily stopped by paper or skin, and are only hazardous if alpha-emitting materials are swallowed or breathed into the body. • Beta particles - are electrons and have a greater penetrating power than alpha particles, but can be stopped by thin layers of water, glass or metal. However, beta emitting material can be hazardous if taken into the body • Gamma and X rays - are electromagnetic radiations. They are very penetrating and heavy shielding materials like lead and concrete are needed to stop them

BIOSAFETY UMMS Exposure Control Plan [http: //inside. umassmed. edu/ehs/manuals/] Key Components • • Guidelines for handling biohazardous agents Emergency/ First Aid Procedures Hepatitis B Vaccination Program Engineering Controls Universal Precautions Work Practice Controls Personal Protective Equipment (PPE)

BIOHAZARD WARNING LABELS Warning labels required on: • Laboratory doors • Containers of regulated waste • Refrigerators and freezers containing blood and other potentially infectious materials • Other containers used to store, transport, or ship blood or other potentially infectious materials Red bags or containers may be substituted for labels

OSHA BLOODBORNE PATHOGEN STANDARD General Industry Standard 29 CFR 1910. 1030 Purpose: Eliminate or minimize employee occupational exposure to human-sourced materials Scope: Covers employees who could be “reasonably anticipated” to come into contact with human blood and OPIM (other potentially infectious materials) Does not cover “Good Samaritan” acts “Universal precautions” means always treating everyone’s blood and other body fluids as infectious. This precaution is governed by the Occupational Safety and Health Administration

BLOODBORNE PATHOGENS Microorganisms such as viruses or bacteria that are carried in blood and can cause disease in people Viruses and related diseases specifically addressed by the OSHA Bloodborne Pathogen Standard include: • Hepatitis B Virus (HBV) • Hepatitis • Hepatitis C Virus (HCV) • Hepatitis • Human Immunodeficiency Virus (HIV) • Acquired Immunodeficiency Syndrome (AIDS)

TRANSMISSION Bloodborne pathogens can be transmitted through contact with infected human or animal blood, unfixed cells, tissues, or organs, or other potentially infectious materials (OPIM) Hazardous OPIM Nonhazardous with Casual Contact w. Cerebrospinal fluid w. Semen w. Vaginal Secretions w. Synovial fluid w. Pleural fluid w. Pericardial fluid w. Human tissues, organs, cells w. Body fluids of UNKNOWN origin should be suspect w. Tears w. Sweat w. Urine w. Feces w. Nasal Secretions Exception: Human bite transmission of HBV with saliva to blood [OR] if blood is present

DISEASE PREVENTION Prophylactic treatment (as available and appropriate; UMMS will provide free of charge) • HBV vaccine • Anti viral / bacterial drugs administered following an exposure

STATE OF MASSACHUSETTS CATEGORIES OF BIOHAZARDOUS WASTE • Human blood, blood products, unfixed cells, tissues, organs, and contaminated items • Contaminated sharps • Cultures & stocks of infectious agents • Human pathological waste • Animal carcasses and/or bedding from animals contaminated with ‘pathogens’

LABORATORY WASTE DISPOSAL SIMPLIFIED

BIOLOGICAL AGENTS REGISTRATION PROGRAM Principal Investigators required to register with the UMMS Institutional Biosafety Committee (IBC) all research projects that involve the use of any of the following: • • • Infectious Bacteria and Viruses Recombinant DNA Microbial toxins (LD 50 <100 mg/kg body wt. ) Human or non-human primate cells, tissues, blood, or blood products DHHS/USDA ‘Select Agents” http: //inside. umassmed. edu/subjects/biosafety/

EXPOSURE INCIDENT If you are exposed to blood or other bodily fluids: 1. Wash are immediately with soap and running water for 20 minutes 2. If cut by an item which has blood on it, try to save the item for contamination testing 3. Report any on the job injuries to their supervisor • Required by HR Policy #06. 05. 13 4. File accident/incident report with Employee Health • Phone: 508 -856 -6263 • Bugs Beeper: Page 77 -2847 (77 -Bugs) 5. Seek immediate attention • • Employee Health Emergency Room

LABORATORY SAFETY TRAINING Required: ü New Employee Orientation-Lab Safety Training q Annual Lab Safety Training (Online) q Department Specific training Job Dependent: q Shipping of Biological & Infectious Material q Shipping of Dangerous & Hazardous Substances (Chemical) q Radiation Safety Training

ANNUAL HEALTH & SAFETY TRAINING (ONLINE) 1) User will receive “invitation” email to complete online Health & Safety training 2) Complete training within 30 days of receiving email 3) Click on link and set up password Questions: Contact EH&S at 508 -856 -3985

THANK YOU! If you have any questions, please contact the EH&S Dept. at 508 -856 -3985