NETCHEM Remote Access Laboratory Guide Determination of preservatives
NETCHEM Remote Access Laboratory Guide • Determination of preservatives and other additives in soft beverages by LC-DAD • • • Remote access: Live connection (in real time) between lab and classroom Teacher: dr Dejan Orčić, associate professor (University of Novi Sad, Faculty of Sciences) Course: Chromatographic Analysis of Food and Supplements ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Aims: The aim of the exercise is to: • provide the students practical skills in operating Agilent 1100 series HPLC instrument and Chem. Station Data. Analysis software, • demonstrate the application of HPLC in food analysis. Outcomes: After succesful completion of the exercise, the student is capable to: • • prepare simple samples for HPLC analysis, prepare Agilent 1100 series HPLC for analysis and conduct the analysis, use Chem. Station Data. Analysis software, prepare a calibration and perform quantitative analysis calculations. ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Background In this exercise, high-performance liquid chromatography with UV detection is used to quantify the content of selected additives in soft beverages. Both sodium benzoate (E 211) and potassium sorbate (E 202) are used as preservatives. According to Serbian regulations, maximum allowable concentration is 300 mg/L of sorbic acid or 150 mg/L of benzoic acid, if only one is present, or 250 mg/L of sorbic acid and 150 mg/L of benzoic acid if they are used in mixture. sodium benzoate potassium sorbate ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Prerequisites • Prior to attending this exercise, students should: • familiarize themselves with lab safety principles (provided at the course's Moodle page) and pass the lab safety test (administered at home institution), • familiarize themselves with basics of HPLC-DAD technique (any general source, such as instrumental analysis textbook, is sufficient), • familiarize themselves with Team. Viewer software* (a guide is provided at NETCHEM page), • familiarize themselves with the lab manual (provided at the course's Moodle page), procedures, instrument and software (movies are provided at the course's Moodle page). * if needed, students can request a trial remote session through forum at course's Moodle page, to receive a practical training in Team. Viewer usage
DESCRIPTION OF REMOTE ACCESS 1. NETCHEM COMMUNICATION SIDES (NOTE: NETCHEM Communication is defined as event that involves all kinds of internet interactions (in real time and not in real time) between participants via devices (PCs, laptops, tablets and mobile phones)) host side (NOTE: Host side of NETCHEM Communication is defined as PC who invites other users to join the session) participant’s PC in laboratory guest side (NOTE: Guest side of NETCHEM Communication is defined as PC who joins the invitation to session) participant’s PC in remote classrooms/laboratories 2. COMMUNICATION SOFWARE Meeting: Yes Team Viewer Remote control: Yes Meeting and Remote control simultaneously: Yes Skype Call 1: 1: No Conference Call: No 3. COMUNICATION HARDWARE on host side 1 PC, 1 headsets with microphone, camera on guest side for each student: 1 PC, 1 headsets with microphone, camera 4. INFORMATION EXCHANGE TYPE Yes Place of Educator participant: host side Educational (one side is dominantly receptive) Number of educator(s): 1 Place of student participant: guest side Number of student participant(s): up to 24 ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Remote Access Connection Instructions V. You will need: a) Computer with broadband internet connection, b) Team. Viewer software (can be run in portable mode, or installed) of the same version as indicated in course's Moodle page; it can be downloaded for free from www. teamviewer. com c) Headset (or speakers and microphone). VI. Steps: a) Open Team. Viewer software and, if preffered, logon to your Team Viewer account. The exact time of remote session, its Meeting ID and password will be provided in advance by course organizers (in course's Moodle page). When joining the meeting, in Your name field, enter your first and last name and (in parentheses) acronym of your institution. b) You should soon see the Remote Access desktop and at this point you can interact with the icons on the screen as if it were your desktop. Switch to full screen mode by selecting the maximize screen option in the top right corner of the screen. c) Follow the teacher's instructions. Refrain from interacting with instrument control software unless specifically instructed to do so by the teacher – you are operating the real instrument, and it can be damaged by mishandling. Behaviour that can damage the instrument or is disruptive can result in the your remote control privileges being revoked, or you being permanently removed from the remote lab. d) Upon completion of the session, move your mouse to the top right corner of the screen, and click on the X to disconnect the remote session. ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Author, Editor and Referee References This remote access laboratory was created thanks to work done at University of Novi Sad, Faculty of Sciences. Contributors to this material were: dr Dejan Orčić (University of Novi Sad, Faculty of Sciences) Refereeing of this material was done by: Anna Warnet (Sorbonne Université, Paris) Editing into NETCHEM Format and onto NETCHEM platform was completed by: dr Dejan Orčić (University of Novi Sad, Faculty of Sciences) ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
References and Supplemental Material The NETCHEM platform was established at the University of Nis in 2016 -2019 through the Erasmus Programme. Please contact a NETCHEM representatives at your institution or visit our website for an expanded contact list. The work included had been led by the NETCHEM staff at your institution. ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
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