NETCHEM Method validation of zinc in wastewater by
NETCHEM Method validation of zinc in wastewater by AAS Part 2 – Accuracy Author: Ph. D Ivana Mihajlović, assistant professor Institution: University of Novi Sad, Faculty of Technical Sciences, Department of Environmental Engineering, Trg Dositeja Obradovića 6, 21000 Novi Sad Course: Methodology of instrumental analysis of water and air ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Background • Educational objectives • To develop skills in statistical evaluation of data to verify the linearity of the calibration curve of the analytical method • To learn how to statistically process data to verify accuracy and precision of the analytical method • To master skills in calculation of LOD and LOQ of the analytical method ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Prerequisites • Students should: • familiarize themselves with basics of AAS technique • familiarize themselves with excel programme • see materials uploaded at the course's (http: //mdl. netchem. ac. rs/course/view. php? id=24) Moodle page
Make an excel sheet – Accuracy - with table with data for statistical calculations ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Enter values for sample concentrations and spiked samples with zinc standard solutions Spiking of water samples with zinc standard solution need to be performed at three concentration levels (for example 0. 1, 0. 5, 0. 8 mg/L) ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Calculate Csp-Cs=C 12 -B 12 ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
R=(Csp-Cs)/Crm*100% ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Calculate Recovery (%) R=D 12/G 12*100 R=(Csp-Cs)/Crm*100% ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Calculate min and max Recovery Rmin = MIN (E 12: E 17) Rmax=MAX(E 12: E 17) ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Calculate min and max Recovery for all three concentration levels of spike Rmin = MIN (G 13, G 20, G 27) Rmax=MAX(G 14, G 21, G 28) ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Calculate deviation of Rmin and Rmax Deviation Rmin = ABS(100 -G 32) Deviation Rmax=ABS(100 -G 33) ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Compare deviation of Rmin and Rmax to check if the requirement for accuracy is fulfilled Deviation <10% ___________________________________________________ This project has been funded with support from the European Commission. This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
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