Natural Products Chemistry Laboratory Fall Semester 2014 Sunday

Natural Products Chemistry Laboratory Fall Semester 2014 Sunday from 8 -12 am , organic chemistry laboratories Chemistry Department, Faculty of Science

Instructors • Dr. Amir E. Wahba, Lab supervisor • Research Interest: • Marine natural products, total synthesis of natural products, cancer chemotherapy, pseudo LDL nanoparticles for targeted delivery of cancer chemotherapy, new synthetic methodologies, medicinal chemistry, isolation and structure elucidation of bioactive natural products. • Contact: • Office hours: Saturday, by appointments • Email: amirwahba@du. eg • Ahmed Sheta, Teaching Assistant (TA) • Hani El-Beheiry , Teaching Assistant (TA)

What are Natural Products?

What are Natural Products?

What are Natural Products? • Living organisms produces natural products for several reasons: • • • Live Grow Reproduce Defense Communicate • Examples of living organisms that produces natural products: • • Bacteria Fungi Plants Marine organisms (sponges, alga. . . etc)

How do plants produces organic compounds? Biosynthesis

Metabolism • Primary Metabolism: All the chemical reactions necessary for the live of the organism by itself. • Primary Metabolites: All the chemical products involved in the Primary Metabolism. • Secondary Metabolites: All the chemical products that are produced by the living organism but are NOT involved in the primary metabolism. Not necessary for the live of the organism by itself but useful for interaction with others (chemical communication, chemical defenses).

What is Natural Products Chemistry? • Two major fields associated with natural products chemistry: • Isolation, purification and structure elucidation of natural products: • Different isolation and purification techniques • Chemical and spectral methods for structure elucidation • Total synthesis of natural products: • Knowledge about different synthetic methodologies • Strong knowledge of stereoselective reactions • Very challenging and difficult field

Course outlines • Course Description: • This course is designed to give students an overview on the different isolation and purification techniques • Learning outcomes: • Students should learn the basics of the different chromatographic techniques as well as writing a well -organized lab note. Knowledge about natural products are expected with the conjunction with the course number. • Textbox and material: All experiments will be available online • Course requirements and grading: • There will be a weekly quiz and lab report that need to be completed and delivered before the beginning of the lab. Additional assignments are also expected specially when there an extra time left in the lab. • The following grading system will be applied: • • • Attendance 10% Lab report 15% Quiz 10% Behavior and responsibility 15% Note Book 10%

Regulations • All students are expected to follow the safety and waste disposal rules. Lab goggles, coats and gloves are mandatory for all students and TA’s. No student will be allowed in the lab without those items. • Labs start 8: 00 am sharp! Please make all your arrangements to be here in time. • Quizzes and lab assignments will be taken at the seminar room in located at the chemistry Department. • A positive attitude and willingness to learn is essential to be successful. • You must come to lab prepared and on time. Read the experiments ahead of time. • Write down questions that come up as you read the experiment. Ask questions. • Although good results are the ultimate goal, determining why an experiment did not work and understanding the experiment is equally or more valuable.

How to write your lab note?

Otto Hahn Nobel Prize in Chemistry, 1944 Radioactivity

Lab Reports • Lab reports will contain the following information: • Introduction: • Provide a short informative paragraph about the chemical being evaluated and how it is used. • Include information that describes the practical application of the compound or procedure in question and a statement of the objective. • There may be more than one objective for each experiment. • Data ( include calculations): • Graphs, tables, spectra and chromatograms are part of the data and must also be included in the report. • Result and Discussion: • Describe the procedure briefly and the results of your experiment. Example: If you separated compounds, tell how it was done. If you synthesized a compound, tell how it was made and in what yield. Support your statements with your data. Data report in the data sections should be referred in the result and discussion. Explain the data/results and what they imply. Discuss unusual observations, errors and anything that will better your understanding of the principles involved. Make suggestions that would solve the stated problems. Make this section brief and concise. • Conclusion: • A brief statement to answer the problem posed in the objective.

• Coffee break

Experiments outline Date Experiment October 12 Introduction and orientation October 19 Isolation of caffeine from tea Liquid-liquid extraction October 26 Isolation of piperine from black pepper Solid-liquid extraction November 2 Isolation of limonene from Distillation oranges Isolation of cholesterol from egg Thin layer chromatography lipids November 9 November 16 Techniques Column chromatography November 23 Isolation of carotenoids from spinach Dyes lab November 30 Dyes lab