NANOPARTICLES AND BLOOD COAGULATION Presentation by Megan Nesland

NANOPARTICLES AND BLOOD COAGULATION Presentation by: Megan Nesland Mentor: Tracy Chuong University of California, Santa Barbara Department of Chemistry and Biochemistry Kick Starting Coagulation with Silica Nanoparticles

THE BIG PICTURE - Trauma injury accounts for 30% of all life years - $406 billion a year, including both health care costs productivity lost in the U. S. and lost - Cause of Death: - #1 for age group 1 -44, or 47% of all deaths in this age range - #3 as leading cause of death overall, across all age groups http: //www. nationaltraumainstitute. org/home/trauma_statistics. html

THE BIG PICTURE http: //www. z-medica. com/first. Responder/Home. aspx http: //commons. wikimedia. org/wiki/File: Syringe_Glove_01. jpg

BLOOD COAGULATION 101 http: //www. biosbcc. net/doohan/sample/htm/Hemostasis. htm

BLOOD COAGULATION 101

WHY NANOPARTICLES? - Surface Area - Potential for customization - Biocompatible

HOW DO SNP (SILICA NANOPARTICLES) WORK? ite s d Diagram courtesy of Anna May and Damien Kudela un o W

QUICKIE QUIZ 1 Looking at the picture below, what would happen to the nanoparticle if you didn’t include PEG? Work with your neighbor to choose 1 answer and explain why you selected it. a. The blood would clot immediately a. The blood would never clot a. The particle would become toxic to the body. a. Nothing- the SNP would work fine. ite s d n u Wo

QUICKIE QUIZ 1 - ANSWER Looking at the picture below, what would happen to the nanoparticle if you didn’t include PEG? Work with your neighbors to choose 1 answer and explain why you selected it. a. The blood would clot immediately a. The blood would never clot a. The particle would become toxic to the body. a. Nothing- the SNP would work fine.

QUICKIE QUIZ 1 - EXPLANATION - SNP has lots of negative charges SNP - Negative charges Blood Clotting - PEG hides the charges until the SNP reaches the wound PEG

QUICKIE QUIZ 2 Write 3 -5 sentences on your own answering the question: Why does the SNP work at the wound site, but not before? Be as specific as possible.

QUICKIE QUIZ 2 - EXPLANATION Write 3 -5 sentences on your own answering the question: Why does the SNP work at the wound site, but not before? Be as specific as possible. - SNP contains a specific peptide - The peptide bonds with an enzyme found only at the wound site - This reaction releases PEG, exposing the negative surfaces. ite ds n u Wo

HOW DO SNP WORK?

HOW DO SNP WORK? Before Clotting . 25 mg/m. L SNP Solution After Clotting

HOW DO SNP WORK? Patch Solution

In Solution: In Patch: SNP

QUICKIE QUIZ 3 Which delivery system of the SNP will cause clotting more quickly. Solution or Patch? Write a claim that answers this question and support it with evidence and reasoning.

QUICKIE QUIZ 3 - ANSWERS - Surface area is key to the function of nanoparticles - Patched particles have less surface area that those in solution - Particles in solution will react more quickly and cause clotting. 72 Surfaces 26 Surfaces

A BIG THANKS TO… Tracy Chuong Frank Kinnaman Damien Kudela Galen Stucky MRL RET Program