Nanonics General SPM 12 01 2003 The Nanonics

Nanonics General SPM 12. 01. 2003 The Nanonics SPM Advantage Standard Atomic Force Imaging at the Highest of Resolutions and Quality Coupled with the Unique Advantages of Integrated Microscopy & Unique Sensor Technology The Hallmark of All Nanonics SPM Systems

Nanonics General SPM 12. 01. 2003 DNA Imaging With The Nanonics NSOM/SPM-100 System & AFM Glass Probes

Nanonics General SPM 12. 01. 2003 Green Monkey Kidney Cells Imaged with Silicon Cantilevers 67. 6 micron scan

Nanonics General SPM 12. 01. 2003 Green Monkey Kidney Cells Imaged with Silicon Cantilevers 35 micron scan

Nanonics General SPM 12. 01. 2003 Imaging Fibronectin Effusing from Green Monkey Kidney Cells 12 micron scan 4. 69 micron scan

Nanonics General SPM 12. 01. 2003 Gold Beads on Anti-Fibronectin Antibodies on Slide Coated with Fibronectin

Nanonics General SPM 12. 01. 2003 Green Monkey Kidney Cells Imaging with Glass NSOM Cantilevers 40 micron scans

Nanonics General SPM 12. 01. 2003 Green Monkey Kidney Cells Imaging with Glass NSOM Cantilevers

Nanonics General SPM 12. 01. 2003 Imaging Budding Yeast Cells in Physiological Media

Nanonics General SPM 12. 01. 2003 Topography & Phase with Silicon Cantilevers

Nanonics General SPM 12. 01. 2003 Polymer PMMA Microspheres Atomic force image of the microspheres. The large 70 micron z scanning range of the Nanonics 3 D Flat Scanning System and the 100 micron or more tip length of the cantilevered optical fiber allows even large topographic alteration to be readily monitored. The image was obtained with a normal force intermittant contact mode technique.

Nanonics General SPM 12. 01. 2003 Polymer PMMA Microspheres AFM Crossection As can be seen the large Z range of the Nanonics 3 D Flat Scanner allows us to readily measure the height of these microspheres.

Nanonics General SPM 12. 01. 2003 <0. 1 MICRON IMAGING IN A 400 MICRON TRENCH

Nanonics General SPM 12. 01. 2003 Investigating Deep Trenches & Side Walls The image at the right of a 2 m deep and 1 m wide silicon trench was obtained with a silicon cantilever. This conventional silicon probe cannot reach the bottom of the trench and the profile obtained reflects a convolution of the tip shape and the feature shape The image at the right of a 10 m deep and 2 m wide silicon trench was obtained with a tapered glass cantilevered AFM probe. These probes with their long probe tips profile such trenches as effectively as carbon nanotube based probes. However, these glass probes are much more robust and easier and cheaper to obtain.

Nanonics General SPM 12. 01. 2003 Side Wall Imaging Glass probes have their probe tip exposed, unlike silicon cantilever probes in which the probe tip is recessed under a silicon cantilever. As a result glass probes can be placed against a side wall and a Z, X image can be performed. Thus, side wall imaging is readily accomplished with on line viewing in transparently integrated optical microscope or scanning electron microscope.

Nanonics General SPM 12. 01. 2003 Multidimensional Functional Imaging AFM Thermal Conductivity 0 V Resistance 70 V

Nanonics General SPM 12. 01. 2003 Kelvin Probe Characterization of Optoelectronic Semiconductor Materials In the Dark In the Light AFM Cantilevered Fiber Probe Light Induced Kelvin Probe Alterations in Observed Normal Force

Nanonics General SPM 12. 01. 2003 Nanopens for Liquid and Gas Delivery Including Bio. Molecule Delivery Cantilevered nanopipette Nanoeteching of chrome by dispensing liquid through a cantilevered force sensing nanopipette, a Nanopen. TM Middle & right frames recorded through the fully integrated optical microscope during the chemical etching process [Appl. Phys. Lett. 75, 2689 (1999)]

Nanonics General SPM 12. 01. 2003 G Protein and GFP Deposited with a Nanopen & Imaged with a Glass AFM Cantilever AFM of Printed GFP 50 nm AFM of Printed G Protein NSOM of Printed GFP