Myeloma plasma cells alter the bone marrow microenvironment

Myeloma plasma cells alter the bone marrow microenvironment by stimulating the proliferation of mesenchymal stromal cells by Jacqueline E. Noll, Sharon A. Williams, Christine M. Tong, Hongsheng Wang, Julie M. Quach, Louise E. Purton, Katherine Pilkington, Luen B. To, Andreas Evdokiou, Stan Gronthos, and Andrew C. W. Zannettino haematol Volume 99(1): 163 -171 January 14, 2014 © 2014 by Ferrata Storti Foundation

Clonogenic CFU-F numbers are elevated in MGUS and myeloma patients. Jacqueline E. Noll et

MSC numbers are elevated in primary stromal cultures from myeloma patients. Jacqueline E. Noll

The number of STRO-1+ALP− MSC correlate positively with plasma cell burden at the time

Human STRO-1+ALP-MSC are a rich source of OC- and myeloma PC-activating factors. Jacqueline E.

MM disease progression is accompanied by changes in the BM mesenchyme in mouse model of MM. Six-week old C 57 BL 6/Ka. Lw. Rij. Hsd mice (n=8) were infused with 5× 105 luciferase/GFPlabeled 5 TGM 1 myeloma PC. Age matched, control mice (n=8) were injected with vehicle. . . Jacqueline E. Noll et al. Haematologica 2014; 99: 163 -171 © 2014 by Ferrata Storti Foundation

Flow cytometric isolation of CB-derived MSC and OB. Bones from C 57 BL 6/Ka. Lw. Rij. Hsd mice were gently crushed and rinsed with PBS to remove the BM. The bone fragments were digested with collagenase and the resultant single cell suspension depleted of mature hem. . . Jacqueline E. Noll et al. Haematologica 2014; 99: 163 -171 © 2014 by Ferrata Storti Foundation