Mycology revision Nada Mohamed Ahmed MT ASCPi Types

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Mycology revision Nada Mohamed Ahmed , MT (ASCP)i

Mycology revision Nada Mohamed Ahmed , MT (ASCP)i

Types of Culture media Type of culture media Name of the Picture culture media

Types of Culture media Type of culture media Name of the Picture culture media NON SELECTIVE Sabouraud dextrose agar (SDA) Selective media Corn meal agar (CMA) Brain Heart Infusion (BHI) agar Bird seed agar Mycosel Agar – special for dermatophytes CHROMagar special for Candida

Microscopic techniques – used to Identify of fungal morphology : Name of techniques Method

Microscopic techniques – used to Identify of fungal morphology : Name of techniques Method wet mount(LPCB) 1 -Teased Preparation gently tease the inverted side of the specimen into the staining (LPAB) fluid. 2 -Scotch tape preparation touch it to the surface of the colony put it on a slide that contains a drop of lactophenol cotton blue like, 3 -Slide Culture Cut the plug; put it on a sterile slide on top of the sterile water agar. . . Picture

DERMATOPHYTOSIS (infection of dermatophytes) DERMATOPHYTOSIS Location Tinea corporis - small lesions occurring anywhere on

DERMATOPHYTOSIS (infection of dermatophytes) DERMATOPHYTOSIS Location Tinea corporis - small lesions occurring anywhere on the body Tinea Pedis – Athlete’s Foot Infection Tinea unguium (onychomycosis) - nails Tinea capitis - head - Tinea barbae - ringworm of the bearded areas of the face and neck. picture

Diagnosis of dermatophytes (depend on Macroconidia shape) dermatophytes Shape of Macroconidia • Trichophyton Cylinder

Diagnosis of dermatophytes (depend on Macroconidia shape) dermatophytes Shape of Macroconidia • Trichophyton Cylinder or pencil • Microsporum Spindle • Epidermophyton Club picture

Types of Superficial Mycoses Causative agent diagnosis 1 -PITYRIASIS VERSICOLOR Malassezia furfur (Lipophilic yeast)

Types of Superficial Mycoses Causative agent diagnosis 1 -PITYRIASIS VERSICOLOR Malassezia furfur (Lipophilic yeast) KOH- Spaghetti and meatballs 2 -TINEA NIGRA Exophiala werneckii Produce melanin black or brown color Skin scrapings with KOH Septate hyphae and yeast Septate pigmented hyphae, and asci; unicellular and fusiform ascospores. septate hyphae that develops into arthroconidia 3 -BLACK PIEDRA Piedraia hortae 4 -WHITE PIEDRA Trichosporon. picture

Other Mycosis: Mycosis(infection) Causative agent diagnosis Subcutaneous Mycosis Madurella mycetomatis Madurella grisea Demonstration of

Other Mycosis: Mycosis(infection) Causative agent diagnosis Subcutaneous Mycosis Madurella mycetomatis Madurella grisea Demonstration of granules(graines) in the infected tissue. Systemic Mycosis Giemsa or Wright stains. - Small, oval yeast like cells, 2 -4µ within mononuclear or polymorphonuclear cells, narrow neck budding. Chest radiograph LPCB - White cottony mycelia with large (820µ) thick walled, spherical spores with tubercles or finger like projections – Tuberculate Macroconidia. Histoplasma capsulatum picture

Candidiasis Candida. albicans Aspergillosis Aspergillus fumigatus KOH wet mount Fungal culture High level of

Candidiasis Candida. albicans Aspergillosis Aspergillus fumigatus KOH wet mount Fungal culture High level of Ig. E and Ig. G against aspergillus. Increased level of eosinophils. Sputum examination and culture. Positive circulating cell wall galactomannans (are polysaccharides ) Chest X-ray.