My Omics Epic a journey of learning collaboration

My Omics Epic (a journey of learning, collaboration, innovation and technology) Thomas R. Sutter, Ph. D. Professor of Biology and Chemistry W. Harry Feinstone Chair of Excellence in Molecular Biology Research and Networking February 27, 2019 University of Memphis

Topics Omics in the Pre-Human Genome Era (April, 2003) Differential Hybridization (How sets of genes affect cell differentiation; cancer causation and prevention) Four people, 3 years Targets$250, 000, for dioxin: genes for plasminogen activator inhibitor-2 and interleukin-1 beta TR Sutter, K Guzman, KM Dold, WF Greenlee Chemical Industry Institute of Toxicology, Research Triangle Park, NC Science 18 Oct 1991: Vol. 254, Issue 5030, pp. 415 -418 DOI: 10. 1126/science. 1925598 All things Omics (computational sciences and data integration) Microarray and Metabolomics Eight people, $50, 000, 3 months (plus 2 years of data analysis!) Kennedy, L. H. , Sutter, C. H. , Carrion, S. L. Tran, Q. T. , Bodreddigari, S. , Kensicki, E. , Mohney, R. P. , and Sutter, T. R. 2013. 2, 3, 7, 8 -Tetrachlorodibenzo-p-dioxin-mediated production of reactive oxygen species is an essential step in the mechanism of action to accelerate human keratinocyte differentiation. Toxicol. Sci. 132: 235 -49.

Central Dogma: Sensitive Scalable Comprehensive Proteomics - Component proteins/levels - Location, modifications, interactions - Function and activity Metagenomics (microbiome) - Community of microorganisms - Changes with state (e. g. disease) - Interaction with metabolome, epithelial barrier, immune system Genomics - Structure (sequence) - Regulation - Function: expression in all of its forms Metabolomics - Large scale small molecule - Metabolites: biochemistry - Flux: metabolism

Sutter Lab Collaborations at UM (30 omic publications) University of Memphis (6 dept) University of Pennsylvania University of Dresden, Germany UTHSC (7 dept) General Electric Johns Hopkins University Metabolon University of Pittsburgh Roche Bioscience, CA Dartmouth Roche, United Kingdom Vanderbilt Fox Chase Cancer Center University of Nebraska NYU AHR research Nrf 2 research F. Hoffman-La Roche, Switzerland Dow Chemical NIH: NIEHS, NTP, NCI; NCTR, ORNL NIH ES 017014 (10) NIH CA 39416 (15) CYP 1 B 1 research Other Total > $15 M GE (4) (3) NIH ES 08148 (5) MRC (4) Roche Biosciences DMAD-17 -03 -0229 (5) JHU CAAT (2)

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