Multiparametric flow cytometry of normal human bone marrow

Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies GEIL-GTLLF 2008 Part one : Leukocyte subsets A colour code is applied trhoughout this atlas: Granulocytes in red Monocytes in green Lymphocytes in purple All other cells, in a region of maturation defined by the exclusion of mature cell types and thus dubbed « bermudes » in cyan I. 1

Combinations Tube 1 : CD 11 b/CD 14/CD 16/CD 45 CD 11 b/CD 14 p. I-5 CD 11 b/CD 16 p. I-6 CD 14/CD 16 p. I-7 Tube 2 : CD 11 b/CD 117/CD 34/CD 45 CD 11 b/CD 117 p. I-9 CD 11 b/CD 34 p. I-10 CD 117/CD 34 p. I-11 Tube 3 : CD 36/CD 71/CD 11 c/CD 45 CD 36/CD 71 p. I-13 CD 36/CD 11 c p. I-14 CD 71/CD 11 c p. I-15 Tube 4 : CD 34/CD 13/CD 33/CD 45 CD 34/CD 13 p. I-17 CD 34/CD 33 p. I. 18 CD 13/CD 33 p. I. 19 Tube 5 : CD 38/HLADR/CD 34/CD 45 CD 38/HLADR p. I. 21 CD 38/CD 34 p. I. 22 HLADR/CD 34 p. I. 23 Tube 6 : CD 38/CD 10/CD 19/CD 45 CD 38/CD 10 p. I. 25 CD 38/CD 19 p. I. 26 CD 10/CD 19 p. I. 27 Tube 7 : HLADR/CD 117/CD 34/CD 45 HLADR/CD 117 p. I. 29 DR/CD 34 p. I. 30 CD 117/CD 34 p. I. 31 Tube 8 : CD 34/CD 56/CD 19/CD 45 CD 34/CD 56 p. I. 33 CD 34/CD 19 p. I. 34 CD 56/CD 19 p. I. 35 Tube 9 : CD 64/CD 24/CD 10/CD 45 CD 64/CD 24 p. I. 37 CD 64/CD 10 p. I. 38 CD 24/CD 10 p. I. 39 Tube 10 : CD 7/CD 117/CD 34/CD 45 CD 7/CD 117 p. I. 41 CD 7/CD 34 p. I. 42 CD 117/CD 34 p. I. 43 Tube 11 : CD 22/CD 10/CD 20/CD 45 CD 22/CD 10 p. I. 45 CD 22/CD 20 p. I. 46 CD 10/CD 20 p. I. 47 Tube 12 : CD 4/CD 2/CD 56/CD 45 CD 4/CD 2 p. I. 49 CD 4/CD 56 p. I. 50 CD 2/CD 56 p. I. 51 I. 2

FLx FLy FLx CD 45 FLy FSC FLy SSC Leukocyte subsets display FLx I. 3

CD 11 b/CD 14/CD 16/CD 45 TUBE 1 Granulocytes Monocytes B T NK CD 11 b/CD 14 x x CD 11 b/CD 16 x x CD 14/CD 16 x x x Lymphocytes Bermudes GM progenitors B lineage progenitors I. 4

FSC-Height CD 45 APC FLy FLx CD 11 b. FITC CD 14 PE CD 11 b. FITC FLx CD 14 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 11 b/CD 14 CD 11 b. FITC I. 5

FSC-Height CD 45 APC FLy FLx CD 11 b. FITC CD 16 PC 7 CD 11 b. FITC FLx CD 16 PC 7 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 11 b/CD 16 CD 11 b. FITC I. 6

FSC-Height CD 45 APC FLy FLx CD 14 PE CD 16 PC 7 CD 14 PE FLx CD 16 PC 7 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 14/CD 16 CD 14 PE I. 7

CD 11 b/CD 117/CD 34/CD 45 TUBE 2 Granulocytes Monocytes B T NK GM progenitors B lineage progenitors CD 11 b/CD 117 x x x CD 11 b/CD 34 x x x x x CD 117/CD 34 x x x Lymphocytes Bermudes I. 8

SSC-Height FSC-Height CD 45 APC FLy FLx CD 45 APC CD 117 PE CD 11 b. FITC FLx SSC-Height FLx CD 45 FLy FSC FLy SSC CD 11 b/CD 117 CD 11 b. FITC I. 9

FSC-Height CD 45 APC FLy FLx CD 11 b. FITC CD 34 PC 5 CD 11 b. FITC FLx CD 34 PC 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 11 b/CD 34 CD 11 b. FITC I. 10

FSC-Height FLy FLx CD 34 PC 5 CD 45 APC CD 34 PC 5 CD 117 PE FLx CD 117 PE CD 34 PC 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 117/CD 34 CD 117 PE I. 11

CD 36/CD 71/CD 11 c/CD 45 TUBE 3 Granulocytes Monocytes B T NK GM progenitors B lineage progenitors CD 36/CD 71 x x x CD 36/CD 11 c x x x CD 71/CD 11 c x x x Lymphocytes Bermudes I. 12

FSC-Height FLy FLx CD 71 PE CD 45 APC CD 71 PE CD 36 FITC FLx CD 36 FITC CD 71 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 36/CD 71 CD 36 FITC I. 13

FSC-Height FLy FLx CD 11 c. PC 5 CD 45 APC CD 11 c. PC 5 CD 36 FITC FLx CD 36 FITC CD 11 c. PC 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 36/CD 11 c CD 36 FITC I. 14

FSC-Height CD 71 PE CD 11 c. PC 5 SSC-Height CD 71 PE FSC FLx CD 45 APC FLy FLy SSC CD 71/CD 11 c CD 45 FLx FLx CD 71 PE I. 15

CD 34/CD 13/CD 33/CD 45 TUBE 4 Granulocytes Monocytes B T NK GM progenitors B lineage progenitors CD 34/CD 13 x x x CD 34/CD 33 x x x CD 13/CD 33 x x x Lymphocytes Bermudes I. 16

FSC-Height FLy FLx CD 13 PE CD 45 PC 7 CD 13 PE CD 34 FITC FLx CD 34 FITC CD 13 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 34/CD 13 CD 34 FITC I. 17

FSC-Height FLy FLx CD 33 APC CD 45 PC 7 CD 33 APC CD 34 FITC FLx CD 34 FITC CD 33 APC SSC-Height FLx CD 45 FLy FSC FLy SSC CD 34/CD 33 CD 34 FITC I. 18

FSC-Height FLy FLx CD 33 APC CD 45 PC 7 CD 33 APC CD 13 PE FLx CD 13 PE CD 33 APC SSC-Height FLx CD 45 FLy FSC FLy SSC CD 13/CD 33 CD 13 PE I. 19

CD 38/HLADR/CD 34/CD 45 TUBE 5 Granulocytes Monocytes B T NK GM progenitors B lineage progenitors CD 38/DR x x CD 38/CD 34 x x DR/CD 34 x x x Lymphocytes Bermudes I. 20

FSC-Height FLy FLx HLADRPE CD 45 ECD HLADRPE CD 38 FITC FLx CD 38 FITC HLADRPE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 38/HLADR CD 38 FITC I. 21

FSC-Height FLy FLx CD 34 PC 5 CD 45 ECD CD 34 PC 5 CD 38 FITC FLx CD 38 FITC CD 34 PC 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 38/CD 34 CD 38 FITC I. 22

FSC-Height FLy FLx CD 34 PC 5 CD 45 ECD CD 34 PC 5 HLADRPE FLx HLADRPE CD 34 PC 5 SSC-Height FLx CD 45 FLy FSC FLy SSC HLADR/CD 34 HLADRPE I. 23

CD 38/CD 10/CD 19/CD 45 TUBE 6 Granulocytes Monocytes B T NK GM progenitors B lineage progenitors CD 38/CD 10 x x CD 38/CD 19 x x CD 10/CD 19 x x Lymphocytes Bermudes I. 24

FSC-Height FLy FLx CD 10 PE CD 45 Cy 7 CD 10 PE CD 38 FITC FLx CD 38 FITC CD 10 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 38/CD 10 CD 38 FITC I. 25

FSC-Height FLy FLx CD 19 Cy 5 CD 45 Cy 7 CD 19 Cy 5 CD 38 FITC FLx CD 38 FITC CD 19 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 38/CD 19 CD 38 FITC I. 26

FSC-Height FLy FLx CD 19 Cy 5 CD 45 Cy 7 CD 19 Cy 5 CD 10 PE FLx CD 10 PE CD 19 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 19/CD 10 CD 10 PE I. 27

HLADR/CD 117/CD 34/CD 45 TUBE 7 Granulocytes Monocytes B T NK GM progenitors B lineage progenitors DR/CD 117 x x x DR/CD 34 x x x CD 117/CD 34 x x x Lymphocytes Bermudes I. 28

FSC-Height FLy FLx CD 117 PE CD 45 Cy 7 CD 117 PE HLADRFITC FLx HLADRFITC CD 117 PE SSC-Height FLx CD 45 FLy FSC FLy SSC HLADR/CD 117 HLADRFITC I. 29

FSC-Height FLy FLx CD 34 Cy 5 CD 45 Cy 7 CD 34 Cy 5 HLADRFITC FLx HLADRFITC CD 34 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC HLADR/CD 34 HLADRFITC I. 30

FSC-Height FLy FLx CD 34 Cy 5 CD 45 Cy 7 CD 34 Cy 5 CD 117 PE FLx CD 117 PE CD 34 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 117/CD 34 CD 117 PE I. 31

CD 34/CD 56/CD 19/CD 45 TUBE 8 Granulocytes Monocytes CD 34/CD 56 CD 34/CD 19 CD 56/CD 19 Lymphocytes Bermudes B T NK GM progenitors B lineage progenitors x x x I. 32

FSC-Height FLy FLx CD 56 PE CD 45 APC CD 56 PE CD 34 FITC FLx CD 34 FITC CD 56 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 34/CD 56 CD 34 FITC I. 33

FSC FLy FLx CD 19 PC 7 FLx CD 34 FITC CD 19 PC 7 CD 45 APC CD 19 PC 7 CD 34 FITC FLx CD 45 FLy SSC-Height FLx FSC-Height FLy SSC CD 34/CD 19 CD 34 FITC I. 34

FSC-Height FLy FLx CD 19 PC 7 CD 45 APC CD 19 PC 7 CD 56 PE FLx CD 56 PE CD 19 PC 7 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 56/CD 19 CD 56 PE I. 35

CD 64/CD 24/CD 10/CD 45 TUBE 9 Granulocytes Monocytes B T NK GM progenitors B lineage progenitors CD 64/CD 24 x x x CD 64/CD 10 x x CD 24 CD 10 x x Lymphocytes Bermudes I. 36

FSC-Height FLy FLx CD 24 PE CD 45 Cy 7 CD 24 PE CD 64 FITC FLx CD 64 FITC CD 24 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 64/CD 24 CD 64 FITC I. 37

FSC-Height CD 45 Cy 7 FLy FLx CD 64 FITC CD 10 Cy 5 CD 64 FITC FLx CD 10 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 64/CD 10 CD 64 FITC I. 38

FSC-Height CD 45 Cy 7 FLy FLx CD 24 PE CD 10 Cy 5 CD 24 PE FLx CD 10 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 24/CD 10 CD 24 PE I. 39

CD 7/CD 117/CD 34/CD 45 TUBE 10 Granulocytes Monocytes CD 7/CD 117 x CD 7/CD 34 CD 117/CD 34 x Lymphocytes Bermudes B T NK GM progenitors B lineage progenitors x x x I. 40

FSC-Height CD 45 Cy 7 FLy FLx CD 7 FITC CD 117 PE CD 7 FITC FLx CD 117 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 7/CD 117 CD 7 FITC I. 41

FSC-Height FLy FLx CD 34 Cy 5 CD 45 Cy 7 CD 34 Cy 5 CD 7 FITC FLx CD 7 FITC CD 34 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 7/CD 34 CD 7 FITC I. 42

FSC-Height FLy FLx CD 34 Cy 5 CD 45 Cy 7 CD 34 Cy 5 CD 117 PE FLx CD 117 PE CD 34 Cy 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 117/CD 34 CD 117 PE I. 43

CD 22/CD 10/CD 20/CD 45 TUBE 11 Granulocytes Monocytes CD 22/CD 10 CD 22/CD 20 CD 10/CD 20 Lymphocytes Bermudes GM progenitors B lineage progenitors B T NK x x x x I. 44

FSC-Height FLy FLx CD 10 PE CD 45 PC 7 CD 10 PE CD 22 FITC FLx CD 22 FITC CD 10 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 22/CD 10 CD 22 FITC I. 45

FSC-Height FLy FLx CD 20 PC 5 CD 45 PC 7 CD 20 PC 5 CD 22 FITC FLx CD 22 FITC CD 20 PC 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 22/CD 20 CD 22 FITC I. 46

FSC-Height FLy FLx CD 20 PC 5 CD 45 PC 7 CD 20 PC 5 CD 10 PE FLx CD 10 PE CD 20 PC 5 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 10/CD 20 CD 10 PE I. 47

CD 4/CD 2/CD 56/CD 45 TUBE 12 Granulocytes Monocytes CD 4/CD 2 CD 4/CD 56 CD 2/CD 56 Lymphocytes Bermudes GM progenitors B lineage progenitors B T NK x x x x I. 48

FSC-Height FLy FLx CD 2 PE CD 45 APC CD 2 PE CD 4 FITC FLx CD 4 FITC CD 2 PE SSC-Height FLx CD 45 FLy FSC FLy SSC CD 4/CD 2 CD 4 FITC I. 49

FSC-Height FLy FLx CD 56 PC 7 CD 45 APC CD 56 PC 7 CD 4 FITC FLx CD 4 FITC CD 56 PC 7 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 4/CD 56 CD 4 FITC I. 50

FSC-Height FLy FLx CD 56 PC 7 CD 45 APC CD 56 PC 7 CD 2 PE FLx CD 2 PE CD 56 PC 7 SSC-Height FLx CD 45 FLy FSC FLy SSC CD 2/CD 56 CD 2 PE I. 51

Published on ELN website July 2008 • Contributors – – – – – Christine Arnoulet Marie Christine Béné Françoise Durrieu Jean Feuillard Chantal Fossat Bernard Husson Hélène Jouault Francis Lacombe Marc Maynadié • Coordination – Francis Lacombe & Marie Christine Béné