Mosaic Analysis Reading 152 155 731 732 lecture
![Mosaic Analysis Reading: 152 -155; 731 -732 & lecture notes Problem set 7 Mosaic Analysis Reading: 152 -155; 731 -732 & lecture notes Problem set 7](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-1.jpg)
![The Drosophila eye Composed of several hundred repeating units known as ommatidia Each ommatidium The Drosophila eye Composed of several hundred repeating units known as ommatidia Each ommatidium](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-2.jpg)
![Drosophila eye development Drosophila eye development](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-3.jpg)
![When given a choice of light preference Visible UV When given a choice of light preference Visible UV](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-4.jpg)
![Drosophila prefers UV Visible UV Drosophila prefers UV Visible UV](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-5.jpg)
![Screens for UV blind flies. Visible UV Screens for UV blind flies. Visible UV](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-6.jpg)
![UV blind flies Defective in rhodopsin that detects UV Missing R 7 (R 7 UV blind flies Defective in rhodopsin that detects UV Missing R 7 (R 7](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-7.jpg)
![In both sev and boss mutants, R 7 is missing because it is transformed In both sev and boss mutants, R 7 is missing because it is transformed](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-8.jpg)
![If Boss is a ligand for the RTK Sevenless, then Boss’s function should be If Boss is a ligand for the RTK Sevenless, then Boss’s function should be](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-9.jpg)
![In 1936 Curt Stern observed twin spots y+ sn / y sn+ In 1936 Curt Stern observed twin spots y+ sn / y sn+](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-10.jpg)
![…and explained the patches by mitotic recombination …and explained the patches by mitotic recombination](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-11.jpg)
![But first a note on how mosaics were used to show the fly eye But first a note on how mosaics were used to show the fly eye](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-12.jpg)
![Analyze ommatidia at the periphery of mutant clone. These will be mosaic, containing both Analyze ommatidia at the periphery of mutant clone. These will be mosaic, containing both](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-13.jpg)
![Irradiate white/+ animals and study mosaic eyes. If invariant lineage, then lineage relationships will Irradiate white/+ animals and study mosaic eyes. If invariant lineage, then lineage relationships will](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-14.jpg)
![If not invariant then there will be no lineage relationships among cells in clone. If not invariant then there will be no lineage relationships among cells in clone.](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-15.jpg)
![Boss expression is restricted to R 8, but Sev is expressed in R 1, Boss expression is restricted to R 8, but Sev is expressed in R 1,](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-16.jpg)
![white can be used as cell autonomous marker to monitor the loss of sev. white can be used as cell autonomous marker to monitor the loss of sev.](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-17.jpg)
![sev is required in the R 7 cell; it functions cell automomously sev is required in the R 7 cell; it functions cell automomously](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-18.jpg)
![Can use P element mediated transposition to insert white on other chromosomes. w-/w-; boss-/boss+ Can use P element mediated transposition to insert white on other chromosomes. w-/w-; boss-/boss+](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-19.jpg)
![boss is required in the R 8 cell; it functions cell nonautomomously boss is required in the R 8 cell; it functions cell nonautomomously](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-20.jpg)
![Ras Ras](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-21.jpg)
- Slides: 21
![Mosaic Analysis Reading 152 155 731 732 lecture notes Problem set 7 Mosaic Analysis Reading: 152 -155; 731 -732 & lecture notes Problem set 7](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-1.jpg)
Mosaic Analysis Reading: 152 -155; 731 -732 & lecture notes Problem set 7
![The Drosophila eye Composed of several hundred repeating units known as ommatidia Each ommatidium The Drosophila eye Composed of several hundred repeating units known as ommatidia Each ommatidium](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-2.jpg)
The Drosophila eye Composed of several hundred repeating units known as ommatidia Each ommatidium has 8 photorecetor cells, 4 cone cells, pigment cells and a sensory bristle.
![Drosophila eye development Drosophila eye development](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-3.jpg)
Drosophila eye development
![When given a choice of light preference Visible UV When given a choice of light preference Visible UV](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-4.jpg)
When given a choice of light preference Visible UV
![Drosophila prefers UV Visible UV Drosophila prefers UV Visible UV](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-5.jpg)
Drosophila prefers UV Visible UV
![Screens for UV blind flies Visible UV Screens for UV blind flies. Visible UV](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-6.jpg)
Screens for UV blind flies. Visible UV
![UV blind flies Defective in rhodopsin that detects UV Missing R 7 R 7 UV blind flies Defective in rhodopsin that detects UV Missing R 7 (R 7](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-7.jpg)
UV blind flies Defective in rhodopsin that detects UV Missing R 7 (R 7 photoreceptor cell that contains UV senesitive rhodopsin)
![In both sev and boss mutants R 7 is missing because it is transformed In both sev and boss mutants, R 7 is missing because it is transformed](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-8.jpg)
In both sev and boss mutants, R 7 is missing because it is transformed into a cone cell wild-type sev- or boss. No R 7
![If Boss is a ligand for the RTK Sevenless then Bosss function should be If Boss is a ligand for the RTK Sevenless, then Boss’s function should be](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-9.jpg)
If Boss is a ligand for the RTK Sevenless, then Boss’s function should be required in a cell other than R 7 and Sev should be required in R 7. Ras
![In 1936 Curt Stern observed twin spots y sn y sn In 1936 Curt Stern observed twin spots y+ sn / y sn+](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-10.jpg)
In 1936 Curt Stern observed twin spots y+ sn / y sn+
![and explained the patches by mitotic recombination …and explained the patches by mitotic recombination](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-11.jpg)
…and explained the patches by mitotic recombination
![But first a note on how mosaics were used to show the fly eye But first a note on how mosaics were used to show the fly eye](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-12.jpg)
But first a note on how mosaics were used to show the fly eye develops. No lineage restrictions? Invariant lineage? Like C. elegans R 1 R 2 R 3 R 4 R 5 R 6 R 7 R 8 And all other combinations! R 3 R 1 R 4 R 7 R 6 R 8 R 4 R 5
![Analyze ommatidia at the periphery of mutant clone These will be mosaic containing both Analyze ommatidia at the periphery of mutant clone. These will be mosaic, containing both](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-13.jpg)
Analyze ommatidia at the periphery of mutant clone. These will be mosaic, containing both wild-type (red) and mutant (white) cells. Mutant clone
![Irradiate white animals and study mosaic eyes If invariant lineage then lineage relationships will Irradiate white/+ animals and study mosaic eyes. If invariant lineage, then lineage relationships will](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-14.jpg)
Irradiate white/+ animals and study mosaic eyes. If invariant lineage, then lineage relationships will be maintained in white clones. R 1 R 2 R 3 R 4 R 5 R 6 R 7 R 8
![If not invariant then there will be no lineage relationships among cells in clone If not invariant then there will be no lineage relationships among cells in clone.](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-15.jpg)
If not invariant then there will be no lineage relationships among cells in clone. R 1 R 2 R 3 R 4 R 5 R 6 R 7 R 8 R 3 R 1 R 4 R 7 R 6 R 8 R 4 R 5 Etc… This is what happens! Any cell can adopt any fate. Cells are specified entirely by cell interactions.
![Boss expression is restricted to R 8 but Sev is expressed in R 1 Boss expression is restricted to R 8, but Sev is expressed in R 1,](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-16.jpg)
Boss expression is restricted to R 8, but Sev is expressed in R 1, R 3, R 4, R 6, R 7, the precursors to the cone cells and mystery cells. 1. Does Boss function in R 8 as predicted by its expression pattern? 2. Does Sev function in R 7, or is signaling to the R 7 cell via Sev more complex?
![white can be used as cell autonomous marker to monitor the loss of sev white can be used as cell autonomous marker to monitor the loss of sev.](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-17.jpg)
white can be used as cell autonomous marker to monitor the loss of sev.
![sev is required in the R 7 cell it functions cell automomously sev is required in the R 7 cell; it functions cell automomously](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-18.jpg)
sev is required in the R 7 cell; it functions cell automomously
![Can use P element mediated transposition to insert white on other chromosomes ww bossboss Can use P element mediated transposition to insert white on other chromosomes. w-/w-; boss-/boss+](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-19.jpg)
Can use P element mediated transposition to insert white on other chromosomes. w-/w-; boss-/boss+ P[white+] w- boss- w- P[w+] boss+
![boss is required in the R 8 cell it functions cell nonautomomously boss is required in the R 8 cell; it functions cell nonautomomously](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-20.jpg)
boss is required in the R 8 cell; it functions cell nonautomomously
![Ras Ras](https://slidetodoc.com/presentation_image_h/37e3ce2d10e100d9b39b01178d7a6cdc/image-21.jpg)
Ras
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Pre reading while reading and post reading activities
Mosaic segmentering
01:640:244 lecture notes - lecture 15: plat, idah, farad
Un r 152
152 регмон
Przedszkole 152 łódź
Re liveri [2006] qca 152
254 rounded to the nearest hundred
Mae 152
Graphics for engineers
Cs 152 stanford
Cs 152 berkeley