MONITORING OF MICROCIRCULATION BY FULL FIELD SPECKLECORRELATION TECHNIQUE
MONITORING OF MICROCIRCULATION BY FULL FIELD SPECKLE-CORRELATION TECHNIQUE IN ANIMAL STUD Maxim Vilensky, Saratov State University, Russia Oxana V. Semyachkina-Glushkovskaya, Saratov State University, Russia Denis A. Alexandrov, Saratov State Medical University, Russia Valery V. Tuchin, Saratov State University, Russia; Institute of Precision Mechanics and Control, Russian Academy of Sciences, RAS, Russia; University of Oulu, Finland Polina A. Timoshina, Saratov State University, Russia Viktor A. Kuleshov, Saratov State Medical University, Russia Igor A. Semyachkin-Glushkovsky, Saratov State University, Russia
Outlines The results of experimental study of full field laser speckle imaging in application to cortex and pancreas microcirculation monitoring of laboratory rats at stress-induced stroke, modeled hemorrhagic pancreatitis and impact of exogenous agents are presented The results of phantom study and nail fold capillary blood flow monitoring are also presented 2
Analysis and computing of speckle images (1) The contrast of speckle-modulated images (2) The intensity of the speckle field (3) Standard deviation of intensity (4) Correlation function of strength fluctuation of scattering light (5) Correlation time of strength fluctuation
Investigated objects 2 – Capillary bundle with scatters flow 1 - Rats microcirculation monitoring 3 – human nail bed microcirculation
Cortex microcirculation monitoring
Experimental setup Schematic diagram of experimental setup: 1. He–Ne laser GN 5 P with the wavelength 633 nm; 2. Optical fiber; 3. Detector (CMOS camera Basler A 602 f) (in the speckle imaging regime); 4. Lens tube of the microscope with microscopic objective (LOMO, 10 x) and 8 element LEDilluminator (central wavelength ~530 nm); 5. Object of study (in the microscopy regime)
Speckles contrast data fluctuation via blood flow 1. Mean contrast values for rats one day after the stress exposure; 2. Mean contrast values for rats immediately after action; 3. Mean contrast values for healthy animals.
Pancreas microcirculation monitoring
The pancreas capillary blood flow studied at different states of the experimental animal
Contrast variations of time-averaged speckles caused by pancreas blood flow velocity reperfusion changes
The pancreas capillary blood flow studied by digital image microscopy at different states of the experimental animal
The pancreas capillary blood flow studied by digital image microscopy at different states of the experimental animal at reperfusion
Phantom particles flow visualisation
Speed-contrast relation ship Speed-contrast relationship for scatters flow; Tube diameter 3 mm (left curves) & 50 mm (right curve)
Nail fold speckle images
Speckle contrast variations due to vessels overcomression Contrast Time, sec
Summary Contrast of time-averaged speckles, used as a diagnostic parameter, characterized by a rather high sensitivity to changes in blood microcirculation in superficial layers of the internals caused by pathological changes or external factors The experimental study of the blood flow velocity dynamics under conditions of stroke in laboratory rats demonstrates high efficiency of the developed instrument and algorithm for data acquisition and processing, implementing the method of fullュ field speckleュ imaging, in monitoring of the blood microcirculation state in the brain cortex, affected by pathological changes or action of agents Monitoring of blood flow during the pancreatitis showed a good correlation between data obtained from speckle-correlometry monitoring and dynamic microscopic imaging studies. No significant changes in blood flow existing after prolonged ischemia (10, 15 min), probably indicate the appearance of the irreversible disorders of pancreas blood flow regulatory mechanism. In
Acknowledgments The research has been made possible by program: Fi. Di. Pro TEKES (40111/11) and grants: RFBR #11 -0200560 -а, #224014; PHOTONICS 4 LIFE of FP 7 -ICT-2007 -2; Projects: #1. 4. 09, #2. 1. 1/4989 and #2. 2. 1. 1/2950 of RF Ministry of Education and Science; RF Governmental contracts: 02. 740. 11. 0879, 14. B 37. 21. 0563, 11. 519. 11. 2035
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