Molecular Imaging System General Layout 600 900 nm

  • Slides: 29
Download presentation
Molecular Imaging System

Molecular Imaging System

General Layout 600 – 900 nm light is used to illuminate fluorescent substances injected

General Layout 600 – 900 nm light is used to illuminate fluorescent substances injected into the subject on the image plane.

Optigrid Functionality • Motors provide 120 o phase shifts and focus – Grid moves

Optigrid Functionality • Motors provide 120 o phase shifts and focus – Grid moves on the x-axis 11. 11 um to complete a phase change – Improves Clarity – Grid moves 2 mm on the z-axis to focus image – 30 line pair/mm on a 10 x 10 mm square (line pair approx. 001 in wide) – Lines spatially modulate the illumination A portion of the Opti. Grid at 200 x y z x

Image Sharpening Provided by Qioptiq

Image Sharpening Provided by Qioptiq

3 D Rendering Provided by Qioptiq

3 D Rendering Provided by Qioptiq

Subsystem Breakdown Imaging Unit Medical Testing Mechanical Lens Mount Phantom Hand Grid Mount Software

Subsystem Breakdown Imaging Unit Medical Testing Mechanical Lens Mount Phantom Hand Grid Mount Software Motor Control Optical Image Capture Image Rendering Lens System

Optical System Review

Optical System Review

Lens Selection Parameters • Focal Length • Magnification • Box Length = 250 mm

Lens Selection Parameters • Focal Length • Magnification • Box Length = 250 mm • Box Height = 200 mm. • Lens size

Computar C mount Alternative Lenses: • Telecentric Lens • Zoom Lens • Simple Lens

Computar C mount Alternative Lenses: • Telecentric Lens • Zoom Lens • Simple Lens

OSLO Simulation by Jeff

OSLO Simulation by Jeff

90 degrees Rear Projection 45 degrees

90 degrees Rear Projection 45 degrees

440 mm Pla ten to L 3 mm ens Len s to G rid

440 mm Pla ten to L 3 mm ens Len s to G rid

Degrees of Motion

Degrees of Motion

Motion Solution

Motion Solution

By Edmund NT 56 -35 by Edmund Construction Aluminum/Stainless Steel Mounting Surfaces Precision ground

By Edmund NT 56 -35 by Edmund Construction Aluminum/Stainless Steel Mounting Surfaces Precision ground Straight Line Accuracy 2µm/25 mm of travel Position Repeatability ± 0. 00005" Configurations X; solid top or thru hole Drives Metric Micrometer: 0. 01 mm graduations/0. 5 mm coarse Fine Screw: no graduations/64 pitch Load Capacity 3 kg normal (horizontal orientation, force against stage) 4. 5 kg Ta (vertical orientation, thrust against drive) 1. 4 kg Tb (vertical orientation, thrust away from spring) Weight 0. 078 kg / axis

Metric Rotary Stages by Edmund Stock No. #55 -028 Coarse Rotation 360° Total Fine

Metric Rotary Stages by Edmund Stock No. #55 -028 Coarse Rotation 360° Total Fine Rotation ± 5° Total Travel/Knob Rotation 70. 2 min Horizontal Load Capacity 3 kg Weight 90 g

250 mm

250 mm

Vendor Part # Price 1 Carestream 1 Qi. Optiq Dovetail Optical Rail 12" Optical

Vendor Part # Price 1 Carestream 1 Qi. Optiq Dovetail Optical Rail 12" Optical Rail 1 Edmund Optics NT 54 -401 99. 75 4 Opti. Grid Holder 1 Qi. Optiq 374352 -000 5 Three-Screw Ring Mounts 95 mm Adjustable Len Mount 12. 5 -76 mm 1 Edmund Optics NT 36 -605 49. 00 6 Small Carrier . 5" Dovetail Carrier 2 Edmund Optics NT 54 -403 55. 00 7 Metric Rotary Stage 60 mm 360 Rotary Stage 2 Edmund Optics NT 55 -028 231. 00 8 1/4 -20 Screws To screw into rails for mounting grid & lens 9 1/16" Black Polyurethane 80 A Durometer, 6" x 6" 1 Mc. Master 8716 K 153 11. 74 10 Loctite 404 Quick Set (46551) rubber adhesive, 1/3 oz bottle 1 Ellsworth 46551 17. 69 11 Rule printed sticker 12" ruled tape 1 Mc. Master 12 Camera Lens Computar C 1 Edmund Optics 90. 00 13 Micro Rotary Stage . 75" Rotary Stage 1 Edmund Optics NT 56 -572 260. 00 14 Aluminum Stock 15 Fiber Optic Light Source 1 Dolan Jenner 16 Grid Transparency 1 Output City - 17 Linear Stage Optional for moving printed transparency Edmund Optics NT 56 -357 210. 00 # Part Description 1 Carestream Box 2 Opti. Grid 3 Quant ity 2 - 4

Software System Capabilities • Consist of three separate programs • • • Kodak Molecular

Software System Capabilities • Consist of three separate programs • • • Kodak Molecular Imaging Software Optigrid Motor Control GUI MATLAB – • Scripts will be developed as necessary for calibration and depth resolved discrimination Optigrid Calibration – A system specific calibration process will be developed – Process will rely on manual coordination of three separate programs • Implement necessary algorithms to achieve depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043

Objective: To mathematically investigate and invoke the necessary algorithms to achieve the depth discrimination,

Objective: To mathematically investigate and invoke the necessary algorithms to achieve the depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043 Bevilacqua, F. , Cuccia, D. , Durkin, A. , Tromberg, B. , “Quantitative analysis and imaging of subsurface heterogeneities using spatially structured illumination, ” 2002.

Phantom Design • Objective: To develop a stable, long-term phantom whose optical properties can

Phantom Design • Objective: To develop a stable, long-term phantom whose optical properties can be predetermined and reproduced, at selected wavelengths, of human biological tissues of the hand, over a spectral range between 400 and 1, 000 nm. • Purpose: – – • Simulate light distributions with a geometry of physical tissue. Initially testing system designs Calibration of optical devices. Create reference measurements of reflective fluorescence. How: – Understand of the key physical and biochemical characteristics of tissue that influence its interaction with light. – Match the absorption coefficient μa, scattering coefficient μs, and the anisotropic coefficient g(λ)

Design of Phantom • 3 components: – Casting material • Polyurethane – Absorbing material

Design of Phantom • 3 components: – Casting material • Polyurethane – Absorbing material • phthalocyanin dye (Epolight 6084) – Scattering agent • Aluminum Oxide (alumina; Al 2 O 3)

Total attenuation coefficient, as a function of concentration, of 0. 954 -mm-diameter micro-spheres.

Total attenuation coefficient, as a function of concentration, of 0. 954 -mm-diameter micro-spheres.