Molecular Imaging of Tumor Microvesicles that Enhance Metastasis

EV-mediated Intercellular Communication Ordinary cellular communication (cytokines, chemokines, hormones…) Protein and RNA transfer by

Imaging EVs In Vivo with a Bioluminescence Resonance Energy Transfer (BRET) Reporter Membrane-anchoring BRET

Imaging Cancer Cell-derived Exosomes and Microvesicles In Vivo In vivo bioluminescence imaging of EVs

Acknowledgment Christopher H. Contag, MSU Richard R. Neubig, MSU Michael H. Bachmann, MSU Jonathan

Slides: 5

Molecular Imaging of Tumor Microvesicles that Enhance Metastasis in a Mouse Breast Cancer Model Ahmed A. Zarea*, Gloria I. Perez*, Benedikt Dolgikh, Matthew P. Bernard, Amelia Mc. Gill, Victoria Toomajian, Lukose K. Thampy, Michael H. Bachmann, and Masamitsu Kanada Institute for Quantitative Health Science & Engineering (IQ) Department of Pharmacology & Toxicology Michigan State University World Molecular Imaging Congress 2020, 10/7 -10/20

EV-mediated Intercellular Communication Ordinary cellular communication (cytokines, chemokines, hormones…) Protein and RNA transfer by extracellular vesicles Raposo et al. , J. C. B, 2013

Imaging EVs In Vivo with a Bioluminescence Resonance Energy Transfer (BRET) Reporter Membrane-anchoring BRET EV reporter 580 nm 450 nm Furimazine Red-e. Nano. Lantern (Re. NL): Suzuki et al. (2016) Nat Commun, 7, 13718. Palmitoylation EV reporter: Lai et al. (2015) Nat Commun, 7029. Weissleder (2001), Nat Biotech

Imaging Cancer Cell-derived Exosomes and Microvesicles In Vivo In vivo bioluminescence imaging of EVs Control Exo-Palm. Re. NL MV-Palm. Re. NL EVs were isolated from 4 T 1 cells stably expressing Palm. Re. NL. 1. 0 x 109 EVs were intravenously injected into BALB/c mice.

Acknowledgment Christopher H. Contag, MSU Richard R. Neubig, MSU Michael H. Bachmann, MSU Jonathan W. Hardy, MSU Bryan D. Kim, UC, Santa Cruz John Ronald, Robarts Research Institute/Western University T. Jessie. Ge, Stanford Ramasamy Paulmurugan, Stanford Sanjiv Sam Gambhir, Stanford Daniel Omar Frimannson, Stanford Andrew Wang, Stanford Matthew D. Sylvester, Stanford Tobi L. Schmidt, Stanford Laura L. Bronsart, Stanford Roger L. Kaspar, Trans. Derm Inc. Manish J. Butte, Stanford A. C. Matin, Stanford Alain Delcayre, Exo. Thera LLC Jing Hung Wang, Stanford Masako Harada, MSU Ophir Vermesh, Stanford Fei Liu, Wenzhou Medical University Stephan Rogalla, Stanford Huanhuan Mahsa He, Sun Yat-sen University Matthew P. Bernard, MSU Alicia Withrow, MSU Brian Hall, EMD Millipore Robert Thacker, EMD Millipore Ray Eby, Particle Metrix Nazar Filonov, Particle Metrix Abstract ID: GA 585 Kanada lab Gloria Perez Ahmed Zarea Elyssa Cox Noelia Barvo Mia Mcgill Benedikt Dolgikh Christopher Ng Jordan Walker Merve Elmastas Sherif Ibrahim Contag lab Victoria Toomajian Lukose Thampy Evran Ural Division of Synthetic Biology Assaf A. Gilad Jens Schmidt Taeho Kim Gabriela Saldana Rita Martin Financial support Start-up fund from Michigan State University