Molecular Cell Biology 5068 Bob Mercer rmercerwustl edu
Molecular Cell Biology 5068 Bob Mercer, rmercer@wustl. edu 362 -6924; 991 -1243
Goals For MCB 5068 1. Obtain a solid foundation of knowledge in cell biology 2. Obtain a working knowledge of available techniques. 3. Be able to critically read and evaluate the scientific literature. 4. Be able to define and investigate a biological problem.
Molecular Cell Biology 5068 http: //mcb 5068. wustl. edu Lectures: Tuesday and Thursday, 8: 30 -10: 00 AM, Cori Auditorium Discussion Section: Wednesday, 3 -4 PM Papers assigned One-page summary/critique expected
Molecular Cell Biology 5068 To Do: Visit website: www. mcb 5068. wustl. edu Sign up for course. Check out Self Assessment homework Visit Discussion Sections: Read “Official” Instructions
Molecular Cell Biology 5068
Discussion Section Techniques Protein purification using GST Fusion CRISPER/Cas 9 gene editing SDS-PAGE/Immunoblotting/Immunoprecipitation Immunofluorescence Sanger Sequencing Real time PCR Proliferation Assay/Brd. U In vivo intracellular p. H determination/p. Hluorin Size exclusion chromatography
TA’s: Kelsy Cotto Kelsy. cotto@wustl. edu Bisiayo Fashemi fashemib@wustl. edu
Grading: 3 in class closed book exams accounting for 75% of grade 25% of final grade based on performance in Discussion Section Last years final grades: 28 A 33 A 30 B+ 13 B 1 C+
- Slides: 8