Molecular biology Transcription UengCheng Yang Institute of Bioinformatics

Molecular biology - Transcription Ueng-Cheng Yang (楊永正) Institute of Bioinformatics National Yang-Ming University YM-Bioinfo

轉型或被淘汰? 由局部看問題 由整體看問題 個別分析 鉅量分析 http: //www. sciencemag. org/cgi/content/full/291/5507/1221/F 1 YM-Bioinfo

Presentation of Life • Life can be described in a multi -dimensional space • The above information is compressed and stored in the one-dimensional “sequence” • To decompress the sequence information, you need to have enough dimensions • Tissue and developmental stages are the most important dimensions. Sequence information decompress Expression level Tissue (spatial) Genes Development (temporal) YM-Bioinfo

the one of most fundamental problems for biological research a • Metabolism • Signal transduction • Biosynthesis of macromolecules (mechanism study) – – Replication Transcription RNA processing Translation YM-Bioinfo

Organizing the known information: Integrating different types of pathways Signal transduction Gene regulatory network Metabolic pathway F 6 P EGF CDK E 2 F PFK F 1, 6 P Glycolysis YM-Bioinfo

Mechanism Studies Factors involved => Components Molecular interaction => Events Order of events => Pathways Pathway interaction => Circuits YM-Bioinfo

Appropriate presentation format is essential for computation [EGFR]+[EGF] <-> [EGF-EGFR]+[EGF-EGFR] <->[(EGF-EGFR)2]<->[(EGF-EGFR*)2]+[GAP]<->[(EGF-EGFR*)2 GAP] [(EGF-EGFR*)2 -GAP]+[Grb 2]<->[(EGFEGFR*)2 -GAP-Grb 2] [(EGF-EGFR*)2 -GAP-Grb 2]+[Sos]<->[(EGFEGFR*)2 -GAP-Grb 2 -Sos] [(EGF-EGFR*)2 -GAP-Grb 2 -Sos]+[Ras-GDP]<>[(EGF-EGFR*)2 -GAP-Grb 2 -Sos-Ras-GDP]<>[(EGF-EGFR*)2 -GAP-Grb 2 -Sos]+[Ras-GTP] [Raf]+[Ras-GTP]<->[Raf-Ras-GTP]<->[Raf*]+[Ras-GTP*] Nature biotechnology 20, 370 -375 YM-Bioinfo

Science. Vol 292. May, 2001 The dream of molecular biologists ? Cell. , 100(1): 57– 70 Review, 2000. PNAS, Vol. 95, 14863 -14868 YM-Bioinfo