Molecular Axon Guidance PC 12 cell then NGF

Molecular Axon Guidance PC 12 cell…. then NGF added NGF is neurotropic and neurotrophic

Guidance Factors Growth Factors

Netrin. . was one of the first neurotropic factors

Findings

Netrin from FP

Netrin from FP, But perpendicular

Netrin in media

Cell locomotion like GCs Cell moves forward by extending filipodia & lamellipodia Focal adhesions are formed Cell is pulled forward

Diffusible Contact dependent

Extracellular matrix

Extracellular matrix Proteins of ECM - collagen

Extracellular matrix Proteins of ECM - proteoglycans

Extracellular matrix Proteins of ECM - fibronectin

Extracellular matrix collagen Proteins of ECM - laminin

Interaction of cells with ECM INTEGRINS inactive

Interaction of cells with ECM INTEGRINS ligands have RGD motif differentiation motility growth survival functions of outside-in signals

Interaction of cells with ECM FOCAL ADHESIONS FA

Interaction of cells with ECM FOCAL ADHESION S

Interaction of cells with cells CELL ADHESION MOLECULES

Interaction of cells with ECM and other cells CELL ADHESION MOLECULES VS ECM ATTACHMENT MOLECULES

Ca 2+ Wnt E-Cadherin Frz II -catenin P p 120 -catenin APC Dsh Axin actin filaments Gsk 3 P Tcf/Lef Extracellular Space

Cadherins as Master Regulators of Morphology • Focus is often on adhesive function: Cadherins as molecular glue AJ’s Type of Cadherin Stabilize others junctions Adhesion Passive Inhibition Rho-GTPases Active Promoter Motility CDKI: p 27/p 21 Wnt Proliferation Cadherins Receptor Signaling EGF FGF PDGF Actin Cytoskeleton Transcriptional/ Nuclear Role -catenin/Wnt P 120/kaiso In nucleus -catenin Rho-GTPases Arp 2/3