Migration assay of cell culture Embryonic development Cancer

Migration assay of cell culture Embryonic development Cancer invasion and metastasis Chemotractant of immune cells Tissue repair Angiogenesis www. biochemweb. org

Boyden chamber migration assay Wound Healing Assay

Boyden chamber migration assay manipulation of foreign genes or Fluorescence labeling Drug treatment or condition medium cell migration through membrane

upper chamber lower chamber membrane with different pore size cell migration through membrane colorimetric observation fluorescent observation Cells fixed and staining with crystal violet

control Treatment Boyden chamber migration assay http: //www. youtube. com/watch? v=6 SON 7 VAA 5 k&feature=results_main&playnext=1&list=PLB 7 ECCD 20439 FEFFC

Under agarose migration Cell chamber agarose Chemo attractant chamber

Wound Healing Assay PDGF 0 hrs PDGF 24 hrs

Cell Migration http: //www. youtube. com/watch? v=3 Wl. APsfm. STw Wound healing http: //www. youtube. com/watch? NR=1&v=pn 2 TOGq. HMr. I&feature=endscreen

Wound healing Assay The role of MMPs in vascular smooth muscle cell migration. Phalloidin stain (red) to show actin and Hoechst stain (blue) for nuclear stain. Johnson C, Fini ME, Galis ZS. 2002. muscle cell (SMC) migration and attachment to extracellular matrix”, FASEB Journal 16(4): A 590.

Wound healing migration Assay Protocal 1. Plate cells on 6 well dish 18 -24 hours before assay 2. Remove culture medium 3. Add treatment medium( control, serum free, PDGF( 1 ng/ml)) control Serum free PDGF 4. Scratch cell from center of the well (Using a (yellow) pipette tip make a straight scratch, simulating a wound) 5. Record cell migration by inverter microscope