Microtubules and polarized growth in Aspergillus nidulans Reinhard

Microtubules and polarized growth in Aspergillus nidulans Reinhard Fischer Max-Planck-Institute for Terrestrial Microbiology

Polarised growth and the Spitzenkörper Myosin Actin MT B. Robertson, Athens GA

Conventional kinesin is required for hyphal extension (Natalia Requena) Wild type Dkin. A Requena et al. , 2001, Mol. Mirobiol. 42: 121 -132

Polarised growth and the Spitzenkörper Myosin Actin MT B. Robertson, Athens GA Conventional Kinesin

Cortical interactions of microtubules: Nuclear migration in S. cerevisiae Num 1 (Aps. A) Maekawa et al. , 2003, EMBO J. 22: 438 -449 Yamamoto et al. , 2003, JCS 116: 4501 -4512

Polarity mutants in S. pombe Dtea 1 and Dtea 2 Browning et al. , 2000, JCB, 151: 15 -27

Microtubule organization in Dtea 1 and Dtea 2 strains WT Dtea 1 Dtea 2 Browning et al. , 2000, JCB, 151: 15 -27 Mata and Nurse, 1997, Cell 89: 939 -949 Tea 1 destabilizes microtubules Tea 2 stabilizes microtubules

Kip. A deletion affects polarized growth (Sven Konzack) Wild type kip. A (tea 2) mutant

Kip. A deletion affects Spitzenkörper position wild type kip. A-deletion

Kip. A is required for microtuble organization in the tip wild type Dkip. A Difference to S. pombe Dtea 2

Kip. A localization GFP Sven Konzack

Microtubule dynamics Xin Xiang, Ron Morris m. RFP 1 -Microtubules GFP-Kip. A is delivered in the tip Morris, Curr. Opin. Cell Biol. 15: 54 -59, 2003 Efimov, Mol. Biol. Cell 14: 871 -888, 2003 Zhang et al. , Mol. Biol. Cell 15: , 2003

Microtubule plus end Kip. A Tea. A? Kip. A Hestermann et al. , 2002

Kip. A and polarized growth Dkip. A Wild type Kip. A Tea. A? Putative Tea. A receptor Conventional Kinesin

Dkip. A and Dtea. A display distinct phenotypes wild type Dkip. A Dtea. A

Microtubule plus end Kip. A Alp. A? Kip. A Does Kip. A transport other cargoes? Hestermann et al. , 2002

Localization of Alp. A in A. nidulans (Cathrin Enke) Mitosis GFP-Alp. A 0 2: 27 GFP-MT m. RFP 1 -Alp. A

Deletion of alp. A WT Dalp. A Curved hyphae 25°C 37°C 42°C

Microtubule plus end Curved hyphae Kip. A Alp. A (XMAP) Nud. F (LIS 1) Cortical protein: Tea. A Hestermann et al. , 2002 No curved hyphae Nud. A (dynein) Cortical protein: Aps. A

Different cortical interactions for nuclear migration and polarized growth Aps. A Nud. A Kin. A Tea. A? Alp. A Nud. F Kip. A

Microtubule polarity in A. nidulans Microtubule tracks are used several times m. RFP-Microtubules GFP-Kip. A

Microtubule organization in A. nidulans Kinesins in A. nidulans 103 137 (240/50) 56 56 123 117 (112/16) (240/29) Interphase Mitosis Interphase after septation 7 (292/42) 285 • Bim. C Mitosis (R. Morris) • Klp. A Mitosis (R. Morris) • Kip. B Microtubule dynamics, Mitosis (P. Rischitor) • Kin. A Tip extension (N. Requena) • Kip. A Directionality (S. Konzack) • Two Unc 104 homologues plus four others to be studied