Microscopes You Microscopes in Depth Fall 2017 Microscopes

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Microscopes & You Microscopes in Depth Fall 2017

Microscopes & You Microscopes in Depth Fall 2017

Microscopes What we use! Magnifies organisms Types: Light Microscopes Electron Microscopes MUCH higher magnification

Microscopes What we use! Magnifies organisms Types: Light Microscopes Electron Microscopes MUCH higher magnification Most powerful! Scanning (SEM) & Transmission (TEM) Magnification – increase in apparent size Resolution –helps with clarity 2

Body Tube Ocular lens (Eyepiece) Nosepiece Objectives Stage Clips Diaphragm Light Arm Stage Coarse

Body Tube Ocular lens (Eyepiece) Nosepiece Objectives Stage Clips Diaphragm Light Arm Stage Coarse Adjustment Fine Adjustment Base Always carry a microscope with one hand holding the arm and one hand under the base.

Structures & Function Microscope Part Eyepiece Function(s) Contains the ocular lens; has a magnification

Structures & Function Microscope Part Eyepiece Function(s) Contains the ocular lens; has a magnification of 10 X Revolving Nosepiece Holds the high- and low- power objective lenses; can be rotated to change magnification. Objective Lenses There are three magnification lens: scanning (4 X), low (10 X), and high power (40 X). Stage Clips Stage Light Source Diaphragm Holds the slide in place. Supports the slide as it is being viewed. Projects light upwards through the diaphragm, the specimen (what you’re looking at), and through the lenses Regulates the amount of light on the specimen.

Structures & Function Pt. 2 Microscope Part Arm Fine Adjustment Knob Coarse Adjustment Knob

Structures & Function Pt. 2 Microscope Part Arm Fine Adjustment Knob Coarse Adjustment Knob Base Function(s) Supports the entire microscope. Moves the stage slightly to sharpen the image. Moves the stage up and down for focusing. Used to support the microscope when carried.

CHECK FOR UNDERSTANDING! What knob would you use to enhance magnification? COARSE ADJUSTMENT KNOB

CHECK FOR UNDERSTANDING! What knob would you use to enhance magnification? COARSE ADJUSTMENT KNOB What knob would you use to enhance resolution? FINE ADJUSTMENT KNOB

Comparing Powers of Magnification We can see better details with higher the powers of

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

Calculating Magnification Eyepiece = 10 x Objective Scanning = 4 x (shortest in length)

Calculating Magnification Eyepiece = 10 x Objective Scanning = 4 x (shortest in length) Low Power = 10 x High Power = 40 x (longest) Magnification = Eyepiece x Objective Field of view measured in microns KNOW PARTS OF MICROSCOPE AND WHAT THEY DO!! 9

Band Color Objective Power Eyepiece Lens Power Red 4 x 10 x Yellow 10

Band Color Objective Power Eyepiece Lens Power Red 4 x 10 x Yellow 10 x Blue 40 x 10 x Power of Magnification 40 x 100 x 400 x

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