Microscopes Adapted by Mr Wolf Microscopes Objectives To

Microscopes/ Adapted by Mr. Wolf

Microscopes Objectives: • To name the parts of the microscope and describe their functions

Microscopes Objectives: • To name the parts of the microscope and describe their functions • To describe how to properly use a compound microscope.

Microscope Parts A. B. C. D. E. F. G. H. I. J. K. L. M. Ocular Body tube Stage clip Revolving nose piece Objective Arm Stage Diaphragm Lever to move stage clip Course adjustment Fine adjustment Light source Base

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support Stage - support

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support Stage - support Coarse adjust – general focus

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support Stage - support Coarse adjust – general focus Fine Focus – high power focus

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support Stage - support Coarse adjust – general focus Fine Focus – high power focus Base - support

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support Stage - support Light source Coarse adjust – general focus Fine Focus – high power focus Base - support

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support Stage - support Diaphragm – adjusts amount of light Light source Coarse adjust – general focus Fine Focus – high power focus Base - support

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Arm-Support Stage - support Stage clips – holds slide Diaphragm – adjusts amount of light Light source Coarse adjust – general focus Fine Focus – high power focus Base - support

Label the parts on your microscope picture. Objective Lenses Scanning = 4 X Eyepiece/ocular lens magnification = 10 X Arm-Support Low Power = 10 X High power = 40 X Stage clips – holds slide Diaphragm – adjusts amount of light Light source Stage - support Coarse adjust – general focus Fine Focus – high power focus Base - support

Label the parts on your microscope picture. Eyepiece/ocular lens magnification = 10 X Revolving nosepiece Objective Lenses Scanning = 4 X Arm-Support Low Power = 10 X High power = 40 X Stage clips – holds slide Diaphragm – adjusts amount of light Light source Stage - support Coarse adjust – general focus Fine Focus – high power focus Base - support

Magnification of a Compound Microscope • Because you are looking through multiple lenses the lenses have a “compounding” effect. • The eyepiece always magnifies 10 X Eyepiece Scanning lens (red) Low Power (yellow) 10 X Objective Lens Magnification High Power (blue) 10 X

Magnification of a Compound Microscope • Each of the objective lenses have their own magnification Scanning lens (red) Low Power (yellow) Eyepiece Objective Lens 10 X 10 X 40 X High Power (blue) 4 X 10 X Magnification

Magnification of a Compound Microscope • You then multiply the eyepiece with the objective lens to determine the total magnification Scanning lens (red) Low Power (yellow) High Power (blue) Eyepiece Objective Lens 10 X 4 X Magnification 40 X 10 X 100 X 10 X 400 X

Other Laboratory Techniques l l Staining Centrifugation – A device that can spin tube – Dyes are added to slides to bring out detail and stain certain features. l up to 20, 000 X/min. This is used to separate samples (I. e. parts of cells Cell Culture – Cells of a particular kind are grown on plates with all their required nutrients.

Limits of Resolution • As we focus the microscope to higher powers we see more and more detail, until a certain point. Then objects become blurry and detail is lost. Think of blowing up a picture too big. • Click here to zoom • Resolution is out ability to distinguish two points as separate. • Electron Microscopes have a much higher limit of resolution.

General Procedures • · Make sure all your supplies are out of the way before you get a microscope! Always carry the microscope with one hand on the Arm and one hand on the Base. • · Wear your glasses, the microscope will focus to your eyesight! • · Keep both eyes open, your brain will learn to ignore the other eye.

Carrying a Microscope YES A NO! B

Preparing a slide • • • Using a pipet or dropper, add a drop of water or another solvent to a clean microscope slide. Then, place the specimen in the water. Place the edge of a coverslip on the slide so that it touches the edge of the water. Slowly lower the coverslip to prevent the formation of air bubbles.

Focusing a Specimen l Always start on low power or scanning.

Focusing a Specimen l Always start on low power or scanning. l Focus the specimen using the course adjust.

Focusing a Specimen l Always start on low power or scanning. l Focus the specimen using the course adjust. l Re-center. If high power is needed, turn only after re-centering!

Focusing a Specimen l Always start on low power or scanning. l Focus the specimen using the course adjust. l Re-center. If high power is needed, turn only after re-centering! l Now use the FINE FOCUS ONLY for your final adjustments.

Making a Wet Mount 1. Gather a very thin slice/piece of whatever your specimen is. All samples should be paper thickness or thinner! 2. Place ONE drop of water directly over the specimen. 3. Place the cover slip at a 45 degree angle with one edge touching the water drop, and let go.

Making good drawings 1. Don’t even think of starting your drawing unless you have a pencil (colored are even better) drawings in pen are unacceptable. This is for two reasons: (a) You can erase pencil! (b) You can shade in areas more easily in pencil 2. In the upper left hand corner of each circle include the specimen name as written on the slide label. In the upper right hand corner, include the magnification (100 x or 430 x). 3. Label Everything you identify! Cheek cell 100 X Cell membrane nucleus

Storing The Microscope • Return the lowest power objective in place • Wrap the cord around the base • Return dustcover

Student handouts l Microscope notes page

Microscopes Microscope Parts Label with structure and function Magnification of Lenses Scanning lens (red) Low Power (yellow) High Power (blue) Eyep iece Objectiv e Lens Magnifica tion 10 X 10 X