Microscope Parts of a Microscope Eyepiece Focus Adjustment

Microscope

Parts of a Microscope Eyepiece Focus Adjustment Nose Piece Objective Lens Mechanical Stage Illumination

Vocab Eyepiece: The lens the viewer looks through to see the specimen. Focus Adjustment: Brings the specimen into general focus. Does this by moving the stage up and down. Nosepiece: A rotating turret that houses the objective lenses. The viewer spins the nosepiece to select different objective lenses. Objective Lens: The lens that is closest to the specimen. Magnification of 4 X, 10 X, and 40 X. Mechanical Stage: The flat platform where the slide is placed. Illumination: The light source for the microscope, mirrors to reflect light from an external source up through the bottom of the stage.

How to use a Microscope Turn the nose piece so that the lowest power objective lens is clicked into position. Place the microscope slide on the stage and fasten it with the stage clips. Look at the objective lens and the stage from the side and turn the focus knob so the stage moves upward. Move it up as far as it will go without letting the objective touch the coverslip. Look through the eyepiece and move the focus knob until the image comes into focus. Move the microscope slide around until the sample is in the center of the field of view. Use the focus knob to place the sample into focus. When you have a clear image of your sample with the lowest power objective, you can change to the next objective lenses. You might need to readjust the sample into focus. If you cannot focus on your specimen, repeat steps 3 through 5 with the higher power objective lens in place. Do not let the objective lens touch the slide! When finished, lower the stage, click the low power lens into position and remove the slide.

Need to know! You should always start with the course objective lens before the fine objective lens to adjust the focus on the speciment. Never let the objective lens touch the slide/stage!