Microscope Parts and functions What do microscopes do

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Microscope Parts and functions

Microscope Parts and functions

What do microscopes do? • Enlarge the view of an object/specimen so we are

What do microscopes do? • Enlarge the view of an object/specimen so we are able to observe the details

Compound Light Microscope • more than 1 lens

Compound Light Microscope • more than 1 lens

Compound Light Microscope • Light projected upward to see the specimen

Compound Light Microscope • Light projected upward to see the specimen

Eyepiece / Ocular Lens • lens you look through • lens closest to your

Eyepiece / Ocular Lens • lens you look through • lens closest to your eye

Eyepiece / Ocular Lens agnifies the image 10 X

Eyepiece / Ocular Lens agnifies the image 10 X

Objective Lenses • Closest to the slide / specimen • 3 objectives on your

Objective Lenses • Closest to the slide / specimen • 3 objectives on your scope

Objective Lenses • Scanning lens: Red (4 X), shortest • Low power lens :

Objective Lenses • Scanning lens: Red (4 X), shortest • Low power lens : Yellow (10 X), = • High power lens: Blue (40 X), longest

Scanning Objective Lens • magnifies 4 X • shortest lens • largest field of

Scanning Objective Lens • magnifies 4 X • shortest lens • largest field of view • Red

Low Power Objective Lens • Magnifies 10 X • Medium length • Yellow

Low Power Objective Lens • Magnifies 10 X • Medium length • Yellow

High Power Objective Lens • magnifies 40 X • longest lens • Smallest field

High Power Objective Lens • magnifies 40 X • longest lens • Smallest field of view • Blue

Coarse Adjustment Knob • Moves stage the most, rapidly • ONLY used to focus

Coarse Adjustment Knob • Moves stage the most, rapidly • ONLY used to focus with scanning or low power • A. k. a. ”coarse focus”

Coarse Adjustment Knob • NEVER use coarse focus with high power!!!!!

Coarse Adjustment Knob • NEVER use coarse focus with high power!!!!!

Fine Adjustment Knob • moves stage very slowly • used for fine focusing •

Fine Adjustment Knob • moves stage very slowly • used for fine focusing • May be used with all lenses.

Fine Adjustment Knob • The only knob used when on high power • A.

Fine Adjustment Knob • The only knob used when on high power • A. k. a. “fine focus”

Stage • where all the action takes place • where slide rests

Stage • where all the action takes place • where slide rests

Stage Clips –hold slide in place

Stage Clips –hold slide in place

Revolving Nosepiece • rotates to change objective lenses • be sure lens “clicks” into

Revolving Nosepiece • rotates to change objective lenses • be sure lens “clicks” into position

Body Tube • connects eyepiece + revolving nosepiece (objective lenses)

Body Tube • connects eyepiece + revolving nosepiece (objective lenses)

Body Tube holds lenses the right distance apart

Body Tube holds lenses the right distance apart

Arm • used to carry scope • holds body tube + lenses in place

Arm • used to carry scope • holds body tube + lenses in place over stage

 • Supports the scope • Used to carry the scope Base

• Supports the scope • Used to carry the scope Base

Diaphragm • regulates how much light passes through the slide and into the scope

Diaphragm • regulates how much light passes through the slide and into the scope

Field of View How much of an object/specimen you are able to see through

Field of View How much of an object/specimen you are able to see through the microscope

CALCULATING TOTAL MAGNIFICATION: Eyepiece magnification x Objective magnification = TOTAL MAGNIFICATION

CALCULATING TOTAL MAGNIFICATION: Eyepiece magnification x Objective magnification = TOTAL MAGNIFICATION

What do I do if my view is dark? 1. Check the plug/light switch!

What do I do if my view is dark? 1. Check the plug/light switch! 2. Check the nosepiece! 3. Check the diaphragm!