Microscope Light Microscope Microscope is an instrument used

Microscope

Light Microscope • Microscope is an instrument used to see images that are too small for the naked eye. • Zacharias Janssen invented the first compound light microscope Compound light microscope uses multiple lenses to observe and focus a specimen.

Hooke • Robert Hooke used a compound light microscope to observe cork. He named his observation CELLS.

Light Microscope • Leeuwnhoek invented the first single lens microscope. • He observed and drew single-celled organisms in pond water. Single lens microscope

Compound Light Microscope

Inverted Image 1. Result of light rays 2. Light passes through slide on the stage and focuses towards the objective lens. 3. Light rays cross over 4. The image flips.

Objective Lenses • Important due to resolution- ability to distinguish between two objects. • Eyepiece Magnification is 10 x • Objective Lenses are 4 x, 10 x, 40 x • Total Magnification is found by multiplying the eyepiece magnification and the objective magnification • 10 x times 4 x = 40 x

Rules of Microscope Use • When removing or adding slide always be on low power. • Start with low power and move up – use coarse adjustment. • Higher powers only use fine adjustment • May need to increase light as you move up in magnification • Keep in mind objects are inversed by the microscope – so moving left is really moving right