Microfluidic Cell Culture Array Philip Lee Paul Hung

Microfluidic Cell Culture Array Philip Lee Paul Hung Advisor: Luke Lee © 2005 University of California Prepublication Data Spring 2005

Proposed System Multiplexed Fluidic Control System Microfluidi c Culture Array Connections to Pump Cell Culture Array Objective Lens Environment Control Module ITO Heater Optical Scanner © 2005 University of California Prepublication Data Spring 2005

Microfluidic Culture Unit Design Cell/Reag ent Flow Cells Perfusio n Flow Novel Unit Design Enables: q Two Dimensions of 3 D Schematic of Channel Layers Flow q Cell Localization q Multiplexed Arrays © 2005 University of California Prepublication Data Spring 2005

Fabrication: Soft Lithography SU-8 Silicon Spin coat 2 µm SU-8 for perfusion layer on silicon wafer Photolithography of perfusion layer SU-8 Photolithography of perfusion layer SEM of Single Unit Photolithography of perfusion layer PDMS Glass ITO Mold with PDMS Remove PDMS, punch fluidic connects, bond to glass slide, and mount ITO heater © 2005 University of California SEM Prepublication Data Spring 2005 DIC

Cell Growth 1. 5 days 3 days 7. 5 days q He. La (human carcinoma) cells cultured q Growth rate similar to traditional methods q Maintenance of high density © 2005 University of California Prepublication Data Spring 2005 cultures

Cell Number © 2005 University of California Prepublication Data Spring 2005