Microbiology Tests What do They Mean Shawna Dereemer
Microbiology Tests What do They Mean? Shawna Dereemer, MT(ASCP) Interim Laboratory Administrator Laboratory Preparedness Supervisor
Objectives • Summarize various microbiology testing methods • List components of a laboratory report • Interpret various microbiological test results • Summarize antibiotic susceptibility testing and results
Wyoming Public Health Laboratory
Infectious Disease • Bacterial • Viral • Parasitic
Supporting Laboratory Results • Viral • Identification of virus • Normal or Decreased WBC Count • Increased Lymphocyte Count • Parasitic • Identification of parasite • Increased Eosinophil Count • Bacterial • • • Identification of bacteria Increased WBC Count Increased Neutrophil Count Left-shift in Neutrophil Cell Line Increased CRP Increased Sed-Rate
Laboratory Reports
Bacterial Culture • Grow and isolate bacteria • Various media • Identify a pathogen • Pros • Identify the isolate • Very specific • Cons • Lengthy • Normal flora
Biochemical Testing
Identification
Microbiology Standards
Antimicrobial Susceptibility Testing • Determine antibiotic susceptibility or resistance P. aeruginosa Ceftazidime 19 mm Intermediate
Antimicrobial Susceptibility testing
Antimicrobial Susceptibility Testing
Antimicrobial Susceptibility Testing P. aeruginosa 0. 125 mg/dl Susceptible
Antimicrobial Susceptibility testing
Antimicrobial Susceptibility Testing
Molecular Testing • DNA Probes • DNA Sequencing • Nucleic Acid Sequencing (16 s) • Whole Genome Sequencing (WGS or NGS) • Nucleic Acid Amplification Testing (NAAT) • Polymerase Chain Reaction (PCR) • Ligase Chain Reaction (LCR) • Transcription Mediated Amplification (TMA)
PCR
DNA Sequencing (16 s) • Unusual isolates • Automated instrumentation will not identify • Low confidence levels
Whole Genome Sequencing
Nucleic Acid Amplification Testing (NAAT) • Chlamydia/Gonorrhea testing • M. tuberculosis testing
Virology • PCR • No culture isolation
Parasitology
Immunology/Serology • Chlamydia/GC testing • HIV • Hepatitis B & C
Immunology/Serology • Quantaferon (IGRA) • MMR status (measles, mumps and rubella) • Varicella (chicken pox) • Syphilis (RPR)
Water Lab • EPA Water Lab • Total Coliform • E. coli • New environmental testing coming soon
Questions
Contact Information Shawna Dereemer, MT(ASCP) Interim Laboratory Administrator Laboratory Preparedness Supervisor shawna. Dereemer@wyo. gov 307 -777 -3735
References • M 100 Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute, 28 th Edition, January 2018.
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