MICHAEL HOOKER MICROSCOPY Core Facilityopen to all UNC
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MICHAEL HOOKER MICROSCOPY • Core Facility—open to all UNC investigators • • Confocal – fluorescence/reflection/frap/fret Atomic Force Microscope Laser Micro-Dissection Light Microscopy – tans. /fluor. /DIC/phase/multimode/time-lapse http: //mhmicroscopy. med. unc. edu Mike Chua 843 -3268
MICROSCOPY SERVICES • • • UNC Core facility Confocal Laser Capture Microscopy Electron Microscopy Light Microscopy http: //www. med. unc. edu/microscopy/ Bob Bagnell 966 -2413
FLOW CYTOMETRY CORE • • UNC Core Facility Deconvolution System (3 D) Laser Scanning Cytometer FACS http: //flowcytometry. med. unc. edu/ Larry Arnold 966 -1530
R. SUPERFINE - T. SALMON LABS • • • Scanning Electron Microscopy Transmitted Electron Microscopy Atomic Force Microscopy 3 D Force Microscopy Confocal Microscopy Light Microscopy rsuper@physics. unc. edu tsalmon@email. unc. edu
RUSSELL TAYLOR- VISUALIZATION LAB • • • Scientific Visualization Distributed Virtual Worlds Haptic Display Interactive 3 D Computer Graphics UNC NIH National Research Resources for Computer Integrated Systems for Microscopy and Manipulation-http: //www. cs. unc. edu/Research/nano/cis mm/
UNC NEUROIMAGING LABORATORY G. Gerig, A. Belger, J. Lieberman, J. Piven • Cognitive Neuroscience – ERP lab: Functional brain mapping – Functional brain mapping using f. MRI • Analysis of MRI diffusion tensor imaging (DTI) • 3 D ultrasound: Quantitative image analysis • Developmental Neuroimaging: UNC-NDRC Core • High-power computer system dedicated to neuroimage analysis Neurolab: http: //zeus. ia. unc. edu/ NIRL: http: //www. nirl. unc. edu/ NDRC: http: //www. fpg. unc. edu/~ndrc/cores. cfm
BARRY WHITSEL LAB • Intrinsic Optical Signal Imager (cell-based, in vivo) bwhitsel@med. unc. edu
BIOLOGY Kerry Bloom – Ted Salmon – Tony Perdue • Spinning disk Confocals • Laser Scanning Confocals • Transmission Electron Microscopy kerry_bloom@unc. edu tsalmon@email. unc. edu tdperdue@email. unc. edu
BIOMEDICAL ENGINEERING • • • MRS Metabolomics Imaging (J. Mac. Donald) Cortical Intrinsic Signal Imaging Multiphoton fluorescence Imaging Transmembrane Potential Imaging Intracellular Calcium Imaging Biophotonics Computer Imaging macdona 1@email. unc. edu
CELL & DEVELOPMENTAL BIOLOGY J. Le. Masters – J. Costello – K. Jacobson • • • Confocal – Single & 2 Photon Cryo – Transmitted Electron Microscopy Scanning Electron Microscopy Optical Tweezers Fluorescence Recovery After Photobleaching lemaster@med. unc. edu mjc@med. unc. edu frap@med. unc. edu
CHEMISTRY • Nuclear Magnetic Resonance • Spectroscopy • Total Internal Reflection Microscopy – Nancy Thompson nit@unc. edu
DENTAL SCHOOL • Transmitted Electron Microscopy • Scanning Electron Microscopy Wallace Ambrose wallace_ambrose@dentistry. unc. edu
PEDIATRICS/EPA—J. CARSON • Freeze Fracture • Transmitted Electron Microscopy • Scanning Electron Microscopy • Light Microscopy jlcarson@email. unc. edu
RADIATION ONCOLOGY Edward Chaney • CT • Segmentation • Registration • 3 D & 4 D Display chaney@med. unc. edu
RADIOLOGY – RESEARCH Weili Lin– Etta Pisano – Stephen Aylward • 3 Tesla Magnetic Resonance Imaging (Lin) • Micro Single-Photon Computed Tomography (Lin) • Diffraction Enhanced Imaging(Pisano) • Computer Aided Diagnosis and Display Lab (CADDLab: Aylward) -- 2 D and 3 D image segmentation and registration --Multi-modal image resgistration weili_lin@med. unc. edu; etta@med. unc. edu; aylward@unc. edu
COMPUTER-ASSISTED SURGERY & IMAGING LAB (CASILAB) Elizabeth Bullitt • Vessel/Tumor Imaging and Analysis • Imaged Guided Surgery bullitt@med. unc. edu
GENE THERAPY CENTER • Optical Imaging (Cryocooled, luminescence and fluorescence) Allison Hawke allison_hawke@med. unc. edu
LINEBERGER COMPREHENSIVE CANCER CENTER • Light Microscopy • Fluorescence Microscopy Steve Oglesbee big_rock@email. unc. edu
NEUROSCIENCES & NEURODEVELOPMENTAL DISORDERS RESEARCH CENTERS Eva Anton - Robert Sealock • Confocals – Single & 2 -Photon • Light Microscopy sealock@med. unc. edu anton@med. unc. edu
VASCULAR BIOLOGY CENTER Cam Patterson • Ultrasound for small animals cpatters@med. unc. edu
MEDICAL IMAGE DISPLAY & ANALYSIS GROUP (MIDAG) • At Comp Sci, Psychiatry, Rad Onc, Radiology, Surgery • Analysis Capabilities • Image Analysis • Image Display • Augmented Reality (Computer Science) • Segmentation • Registration http: //midag. cs. unc. edu
NC STATE UNIVERSITY • BOTANY DEPARTMENT – Light Microscopy, Confocal, Nina Allen, Box 7612, NCSU • John Mac. Kenzie – Coordinator, EM Center john_mackenzie@ncsu. edu • Phillip Russell – Dir, Analytical Instru. Facility Atomic Force & TEM, prussell@ncsu. edu • Michael Dykstra – Dir, Laboratory Advanced Microscopy, TEM & Confocal michael_dykstra@ncsu. edu • VET SCHOOL – X-ray, CT, ultrasound, MRI Ian Robertson, ian_robertson@ncsu. edu
DUKE-UNC BRAIN IMAGING & ANALYSIS CENTER (BIAC) • f. MRI Neuroscience acquisition and analyses http: //www. biac. duke. edu/
POSSIBLE APPLICATIONS (from NIH proposal – T. Van Dyke & B. Tsui) High-resolution pinhole SPECT studies: • Can do a series of images with a single injection depending upon the length of the half-life of the radionucleotide. • Locating tumors and bone metastases micro. PET studies: • Locating a large variety of primary tumors and their metastases
POSSIBLE APPLICATIONS (from NIH proposal – T. Van Dyke & B. Tsui) MRI and MRS studies: • Tumor growth • Changes in tumor vascularity Optical Imaging studies: • Luciferase localization • Reporter gene expression Micro. CT studies: • Tumor growth
FUTURE DEVELOPMENTS (from NIH proposal – T. Van Dyke & B. Tsui) • Use Animal Model systems for refining specific imaging technologies—i. e. technologies to measure blood flow and microvascular permeability within tumors. • Imaging results compared to histologic analyses can help develop technologies to measure disease progression and then be used to screen development of disease in backgrounds with additional genetic alterations.
- Michael hooker research center
- Abel hooker
- Name a point that is collinear with the given points
- The brittle, rocky outer layer of earth
- The strong lower part of the mantle
- What are the 3 main layers of the earth? *
- Core rigidities
- Disadvantage of phase contrast microscopy
- Selective medium for vibrio cholerae
- Spermatozoa in urine
- Advantages of scanning probe microscopy
- Provider-performed microscopy test examples
- Fazekas scale
- Branches of microscopy
- Pyuria leukocytes urine
- What is forensic microscopy
- Data recovery techniques
- Glioblastoma multiforme
- Confocal fluorescence microscopy
- Dic phase contrast
- Bright field microscopy
- 1674 microscopy
- Photo emission microscopy failure analysis
- Multiphoton microscopy principle
- Compound microscope use
- Light sources for fluorescence microscopy
- Cvc lung