Methods of Lighting Bio 498698 Lighting Basics Equipment

Methods of Lighting Bio 498/698

Lighting Basics �Equipment �Dynalite �Fiber optics �Modeling lights �Sol. Mate �Light Pad �Moving Modeling Lights �Adjusting the intensity on the Dynalite Flash Powerpack �Additional Equipment Used

Equipment Back Lighting Pad Fiber Optic Illumination System Dynalite Magnetic Articulated Arms Sol-Mate Fiber Optics Modeling Lights w/ umbrellas

Lighting Basics �Modeling/studio Lights: �Modeling Lights can be physically moved �The positions the lights are moved to depends on the specimen, the camera, the lens, the shape and size of the room. �In some cases the modeling lights must be moved for individual specimens (Coleoptera, etc. ) �In some cases the modeling lights can stay in the same position for multiple specimens (Lepidoptera, etc. )

Basic Techniques �Oblique Illumination �Transmitted Illumination �Photographing Reflective Objects

Backgrounds 18% Grey paper backgrounds Overused Correct Pedestal with Grey background paper Wrong Side (The pedestal is where specimens can be placed) The larger background paper is where stands are placed

Umbrellas allow for light to be bounced onto the specimen. This brightens the image without washing it out. Umbrellas should always be positioned so that the inside is facing the specimen being imaged.

Diffusers (Diffusion Cylinders) Note: By adding a diffuser around the specimen in addition to bounce light for reflective subjects. This technique is used in controlling the reflections on surfaces.

Styrofoam Cutouts

Diffuser with Styrofoam Cutouts

Diffusers (Additional) Studio Light with Diffusers Studio Light without Diffuser

Fiber Optics Tenson Adjustment Receptacle Locking Knob Magnet Switch Locking Lever Magnetic Base Magnetic Articulated Arms Arm w/ Fiber optic light

Fiber Optics Lock Un-Lock

Fiber Optics setup

Back Lighting Pad Used for backlighting specimens Attaches directly to the fiber optics system

Sol-Mate Adjusts light intensity Locks the fiber optics in place Diffuser Adjusts aperture Spotlight Position • Illuminates transparent or translucent objects at 1: 1 to 40 X objectives • Sol-Mate has patents pending

Modeling Lights: Example 1 Note: This is an example of how the modeling lights can be positioned.

Full and Full Intensity (Full Power Variation)

Half and Half Intensity (Full Power Variation)

Quarter and Quarter Intensity (Full Power Variation)

Quarter and Quarter Intensity (Power variation turned down 2 turns)

Quarter and Quarter Intensity (Modeling Lights Moved) Modeling Lights Example Position 2

Tricks and Hints �For higher magnifications (i. e. 65 mm, K 2), lighting intensity will decrease �For lower magnifications (100 mm and 50 mm), lighting intensity will increase �Changing the apertures will also influence lighting intensity. A lower f-stop yields increased lighting intensity, as such a higher f-stop yields lower intensity.

Conclusion �Lighting is important for the imaging process and will require adjustments and knowledge of different techniques for what works and doesn’t work depending on the specimen and equipment used �It will likely take some trial and error – don’t worry! The magic will happen with enough effort. �Additional support: http: //www. visionarydigital. com/Support. html