Methodology and Project Planning STR 1 Kawashima Methodology

Methodology and Project Planning STR 1 Kawashima

Methodology List down all tasks Group tasks under general activities Make a process flowchart Write a detailed procedure

Isolation of Hydrocarbon-degrading Bacteria from Guimaras Soil Samples Soil samples from various oil-contaminated areas in Guimaras will be obtained and stored in 50 m. L conical tubes at room temperature. To isolate possible hydrocarbondegrading bacteria from each sample, a pinch of soil will be inoculated into 50 m. L of liquid minimal medium (for 1 L: 0. 5 g KH 2 PO 4, 2. 0 g KNO 3, 1. 0 g Mg. SO 4 7 H 2 O, 0. 0012 g Ca. Cl 2 • 2 H 2 O, 0. 002 g Fe. SO 4 • 7 H 2 O) with 50 μL of bunker oil (Petron Corporation, Philippines), and subjected to incubation while shaking at 37ºC at 225 rpm for one week. Once a visible change in turbidity is observed, an applicator stick will be dipped into the suspension culture and streak plated on Luria-Burtani (LB) agar (Pronadisa, Madrid, Spain). One streak plate will be made for each soil sample and incubated overnight at 37°C. Isolated bacterial colonies of different colonial morphologies will be picked and subcultured into separate LB agar slants.

Isolation of Hydrocarbon-degrading Bacteria from Guimaras Soil Samples Soil samples from various oil-contaminated areas in Guimaras will be obtained and Compartmentalize stored in 50 m. L conical tubes at room temperature. To isolate possible hydrocarbondegrading bacteria from each sample, a pinch of soil will be inoculated into 50 m. L of liquid minimal medium (for 1 L: 0. 5 g KH 2 PO 4, 2. 0 g KNO 3, 1. 0 g Mg. SO 4 7 H 2 O, 0. 0012 g Third Person Ca. Cl 2 • 2 H 2 O, 0. 002 g Fe. SO 4 • 7 H 2 O) with 50 μL of bunker oil (Petron Corporation, Philippines), and subjected to incubation while shaking at 37ºC at 225 rpm for one week. Once a visible change in turbidity is observed, an applicator stick will be dipped into the suspension culture and streak plated on Luria-Burtani (LB) agar Future Tense (Pronadisa, Madrid, Spain). One streak plate will be made for each soil sample and incubated overnight at 37°C. Isolated bacterial colonies of different colonial morphologies will be picked and subcultured into separate LB agar slants.

Project Management Planning Scheduling Project Control • Define activities • When? • Identify critical activities • Update progress

Project Management Tools Gantt Chart Network Chart Process Flowchart Project Schedule

Process Flowchart Traveling to Trinoma Viewing of a movie Masticatio n of dinner Commutin g going home

GENERAL description of an activity Each activity is bounded in a RECTANGLE Traveling to Trinoma ARROWS indicate what’s next Viewing of a movie Masticatio n of dinner Commutin g going home

Gantt Chart 16: 00 17: 00 18: 00 19: 00 20: 00 21: 00 22: 00 4 5 6 Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home current 0 1 2 3 Time, hours

Task names take up one row Names are in imperative form 16: 00 17: 00 18: 00 19: 00 20: 00 21: 00 22: 00 4 5 6 Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home current 0 1 2 3 Time, hours Time increments run along the bottom, in hours/days/weeks/months

Bars represent expected duration of the task 16: 00 Shading tells how complete a task is (think percentage) Arrows connect prerequisite tasks 17: 00 18: 00 19: 00 20: 00 21: 00 22: 00 4 5 6 Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home current 0 1 2 3 Time, hours Shaded bars mean finished tasks while unshaded bars are unfinished

This line marks the current time/date 16: 00 Unshaded bars LEFT of the line are behind schedule 17: 00 18: 00 19: 00 20: 00 21: 00 22: 00 4 5 6 Go to Trinoma Get tickets Buy snacks Watch movie Eat dinner Go home current 0 Fully shaded bars left of the line are completed tasks 1 2 3 Time, hours Unshaded bars right of the line are tasks yet to be started

Network Chart A=1 B=2 D=2 E=1 F=1 Code Description Duration (hr) A Go to Trinoma 1 B Get tickets 2 C Buy snacks 1 D Watch movie 2 E Eat dinner 1 F Go home 1