Metalloproteins and Metalloenzymes 3 1 Uptake Storage and

Metalloproteins and Metalloenzymes 3. 1 Uptake吸收, Storage储存 and Transport运输 3. 2 Biological Chemistry of Iron, copper, and Zinc 3. 3 Biological Chemistry of Other Metals

Distribution of Iron, Zinc, and Copper Fe out of a total of 4 -5 g: 60 -70% hemoglobin; 7 -15% ferritin铁蛋白; 3 -5% myoglobin肌红蛋白; 0. 1% catalase过氧化氢酶; 0. 1% cytochromes细 胞色素类 Zn out of a total of 1. 4 -2. 3 g: liver肝 55 mg; kidney肾 55 mg; muscle肌肉 54 mg; heart 33 mg; pancreas胰 29 mg; spleen脾 21 mg; lung肺 15 mg; brain 14 mg; adrenal肾上腺 12 mg; prostate前列腺 102 mg. Cu out of a total of 80 -120 mg: 8 mg in liver; 15 mg in heart, spleen, kidneys, brain, blood; concentrated in the pigment parts of eyes

Protein Carriers in Blood Plasma Carrier Ion Transferrin转铁蛋白 Fe 3+ Ceruloplasmin血浆铜蓝蛋白 Cu 2+ Albumin清蛋白 Zn 2+, Cu 2+ Metallothionein金属硫蛋白 Zn 2+, Cu 2+, Cd 2+, Hg 2+, Co 2+, Pb 2+, Ag + Phosphoproteins Ca 2+ Non required Mg 2+, Na+ , K+

Ion Storage Proteins Ferritin铁蛋白 Metallothineine Ceruloplasmin Serum Albumin Phosphoproteins Ion Fe 3+ Cu 2+, Zn 2+, Heavy Metals Cu 2+, Zn 2+ Ca 2+

Metalloproteins and Metalloenzymes conjugated proteins = protein + prosthetic (cofactor) Holo-enzyme = apo-enzyme + prosthetic (cofactor)

Classification of Metalloproteins Dioxygen Carriers氧载体 Redox Protein氧化还原蛋白 Classification of Metalloenzymes Oxido-reductase 氧化还原酶 Transferase转移酶 Hydrolase水解酶 Lyase裂解酶 Isomerase异构酶 Ligase连接酶

Bioinorganic Chemistry of Iron Oxygen Carriers: hemoglobin血红蛋白, myoglobin肌红蛋白, hemerythrin蚯蚓血红蛋 白 Redox proteins: iron sulfur protein铁流蛋白, cytochrome c细胞色素c, cytochrome b 5细胞 色素b 5, etc. Redox enzymes: cytochrome p 450细胞色素 p 450, peroxidase过氧化物酶, catalase过氧 化氢酶, etc.

Bioinorganic Chemistry of Zinc Hydrolysis Enzymes - Lewis Acid carbonic anhydrase碳酸酐酶 carboxypeptidase羧肽酶 alkaline phosphatase碱性磷酸酯酶 proteinase NAD-dependent dehydrogenases alcohol dehydrogenase醇脱氢酶 glutamate dehydrogenase谷氨酸脱氢酶 lactate dehydrogenase乳酸脱氢酶 malate dehydrogenase苹果酸脱氢酶 * Structure Roles

Bioinorganic Chemistry of Copper Oxygen Carriers Hemocyanin血蓝蛋白 Redox Proteins-Electron Transfer Protein Plastocyanin质体蓝素 Azurin天青素 Stellacyanin Oxido-Reductase

Oxido-Reductase Enzymes Superoxide Dismutase超氧化物岐化酶 Tyrosinase酪氨酸酶 Laccase漆酶 Ascorbate Oxidase抗坏血酸氧化酶 Galactose Oxidase半乳糖氧化酶 Dopamine-B-Hydroxylase多巴胺羟化梅 Amine Oxidase胺氧化酶 Urate Oxidase尿酸氧化酶 Cytochrome c Oxidase细胞色素c氧化酶